Photosynthetically active radiation (PAR) is an important environmental cue inducing the production of many secondary metabolites involved in plant oxidative stress avoidance and tolerance. To examine the complex role of PAR irradiance and specific spectral components on the accumulation of phenolic compounds (PheCs), we acclimated spring barley (Hordeum vulgare) to different spectral qualities (white, blue, green, red) at three irradiances (100, 200, 400 µmol m-2 s-1). We confirmed that blue light irradiance is essential for the accumulation of PheCs in secondary barley leaves (in UV-lacking conditions), which underpins the importance of photoreceptor signals (especially cryptochrome). Increasing blue light irradiance most effectively induced the accumulation of B-dihydroxylated flavonoids, probably due to the significantly enhanced expression of the F3'H gene. These changes in PheC metabolism led to a steeper increase in antioxidant activity than epidermal UV-A shielding in leaf extracts containing PheCs. In addition, we examined the possible role of miRNAs in the complex regulation of gene expression related to PheC biosynthesis.

Department of Biology and Ecology Faculty of Science University of Ostrava 710 00 Ostrava Czech Republic

Department of Experimental Plant Biology Faculty of Science Charles University 128 00 Praha Czech Republic

Department of Physics Faculty of Science University of Ostrava 710 00 Ostrava Czech Republic

Global Change Research Institute Czech Academy of Sciences 603 00 Brno Czech Republic

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Bridging the Gap: From Photoperception to the Transcription Control of Genes Related to the Production of Phenolic Compounds

Light Drives and Temperature Modulates: Variation of Phenolic Compounds Profile in Relation to Photosynthesis in Spring Barley

What Do We Know about Barley miRNAs?