Cyanobacterial taxonomy is facing a period of rapid changes thanks to the ease of 16S rRNA gene sequencing and established workflows for description of new taxa. Since the last comprehensive review of the cyanobacterial system in 2014 until 2021, at least 273 species in 140 genera were newly described. These taxa were mainly placed into previously defined orders and families although several new families were proposed. However, the classification of most taxa still relied on hierarchical relationships inherited from the classical morphological taxonomy. Similarly, the obviously polyphyletic orders such as Synechococcales and Oscillatoriales were left unchanged. In this study, the rising number of genomic sequences of cyanobacteria and well-described reference strains allowed us to reconstruct a robust phylogenomic tree for taxonomic purposes. A less robust but better sampled 16S rRNA gene phylogeny was mapped to the phylogenomic backbone. Based on both these phylogenies, a polyphasic classification throughout the whole phylum of Cyanobacteria was created, with ten new orders and fifteen new families. The proposed system of cyanobacterial orders and families relied on a phylogenomic tree but still employed phenotypic apomorphies where possible to make it useful for professionals in the field. It was, however, confirmed that morphological convergence of phylogenetically distant taxa was a frequent phenomenon in cyanobacteria. Moreover, the limited phylogenetic informativeness of the 16S rRNA gene, resulting in ambiguous phylogenies above the genus level, emphasized the integration of genomic data as a prerequisite for the conclusive taxonomic placement of a vast number of cyanobacterial genera in the future.

Biology Centre of the CAS Institute of Hydrobiology Na Sádkách 702 7 370 05 České Budějovice Czech Republic

Faculty of Fisheries and Protection of Waters CENAKVA Institute of Aquaculture and Protection of Waters University of South Bohemia in České Budějovice Na Sádkách 1780 370 05 České Budějovice Czech Republic

Faculty of Science Department of Botany University of South Bohemia Branišovská 1760 370 05 České Budějovice Czech Republic

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Abed, R. M., Garcia-Pichel, F. & Hernández-Mariné, M. 2002. Polyphasic characterization of benthic, moderately halophilic, moderately thermophilic cyanobacteria with very thin trichomes and the proposal of Halomicronema excentricum gen. nov., sp. nov. Arch. Hydrobiol. 177:361-70.

Abreu, V. A. C., Popin, R. V., Alvarenga, D. O., Schaker, P. D. C., Hoff-Risseti, C., Varani, A. M. & Fiore, M. F. 2018. Genomic and genotypic characterization of Cylindrospermopsis raciborskii: Toward an intraspecific phylogenetic evaluation by comparative genomics. Front. Microbiol. 9:306.

Aguilera, A., Gómez, E. B., Kaštovský, J., Echenique, R. O. & Salerno, G. L. 2019. The polyphasic analysis of two native Raphidiopsis isolates supports the unification of the genera Raphidiopsis and Cylindrospermopsis (Nostocales, Cyanobacteria). Phycologia 57:130-46.

Akagha, S. C., Johansen, J. R., Nwankwo, D. I. & Yin, K. 2019. Lagosinema tenuis gen. et sp. nov. (Prochlorotrichaceae, Cyanobacteria): a new brackish water genus from Tropical Africa. Fottea 19:1-12.

Albertano, P., Barsanti, L., Passarelli, V. & Gualtieri, P. 2000. A complex photoreceptive structure in the cyanobacterium Leptolyngbya sp. Micron 31:27-34.

Allewalt, J. P., Bateson, M. M., Revsbech, N. P., Slack, K. & Ward, D. M. 2006. Effect of temperature and light on growth of and photosynthesis by Synechococcus isolates typical of those predominating in the octopus spring microbial mat community of Yellowstone National Park. Appl. Environ. Microbiol. 72:544-50.

Alvarenga, O. D., Rigonato, J., Branco, L. H. Z., Melo, I. S. & Fiore, M. F. 2016. Phyllonema aviceniicola gen. nov., sp. nov. and Foliisarcina bertiogensis gen. nov., sp. nov., epiphyllic cyanobacteria associated with Avicennia schaueriana leaves. Int. J. Syst. Evol. Microbiol. 66:689-700.

Anagnostidis, K. & Komárek, J. 1988. Modern approach to the classification system of cyanophytes. 3- Oscillatoriales. Algol. Stud. Arch. Hydrobiol. Suppl. 50-53:327-472.

Azua-Bustos, A., Zuniga, J., Arenas-Fajardo, C., Orellana, M., Salas, L. & Rafael, V. 2014. Gloeocapsopsis AAB1, an extremely desiccation-tolerant cyanobacterium isolated from the Atacama Desert. Extremophiles 18:61-74.

Bagchi, S. N., Dubey, N. & Singh, P. 2017. Phylogenetically distant clade of Nostoc-like taxa with the description of Aliinostoc gen. nov. and Aliinostoc morphoplasticum sp. nov. Int. J. Syst. Evol. Microbiol. 67:3329-38.

Berrendero Gómez, E., Johansen, J. R., Kaštovský, J., Bohunická, M. & Čapková, K. 2016. Macrochaete gen. nov. (Nostocales, Cyanobacteria), a taxon morphologically and molecularly distinct from Calothrix. J. Phycol. 52:638-655.

Berthold, D. E., Lefler, F. W. & Laughinghouse, H. D. 2021. Untangling filamentous marine cyanobacterial diversity from the coast of South Florida with the description of Vermifilaceae fam. nov. and three new genera: Leptochromothrix gen. nov., Ophiophycus gen. nov., and Vermifilum gen. nov. Mol. Phylogenet. Evol. 160:107010.

Berthold, D. E., Lefler, F. W. & Laughinghouse, H. D. 2022. Recognizing novel cyanobacterial diversity in marine benthic mats, with the description of Sirenicapillariaceae fam. nov., two new genera, Sirenicapillaria gen. nov. and Tigrinifilum gen. nov., and seven new species. Phycologia 61:146-65.

Berthold, D. E., Lefler, F. W., Werner, V. R. & Dail Laughinghouse, H., IV 2020. Johannesbaptistia floridana sp. nov. (Chroococcales, Cyanobacteria), a novel marine cyanobacterium from coastal South Florida (USA). Fottea 20:152-9.

Blondeau, M., Sachse, M., Boulogne, C., Gillet, C., Guigner, J. M., Skouri-Panet, F., Poinsot, M., Ferard, C., Miot, J. & Benzerara, K. 2018. Amorphous calcium carbonate granules form within an intracellular compartment in calcifying cyanobacteria. Front. Microbiol. 9:1768.

Bohunická, M., Mareš, J., Hrouzek, P., Urajová, P., Lukeš, M., Šmarda, J., Komárek, J., Gaysina, L. A. & Strunecký, O. 2015. A combined morphological, ultrastructural, molecular, and biochemical study of the peculiar family Gomontiellaceae (Oscillatoriales) reveals a new cylindrospermopsin-producing clade of cyanobacteria. J. Phycol. 51:1040-54.

Borzì, A. 1914. Studi sulle Mixoficee: I. Cenni generali - Systema Myxophycearum. Nuov. Giorn. Bot. Ital. 2:307-60.

Bourrelly, P. 1970. Les algues d'eau douce. Initiation à la systématique. Tome 3: les algues bleues et rouges, les eugléniens, peridiniens et cryptomonadines. N. Boubée, Paris, 512 pp.

Breinlinger, S., Phillips, T. J., Haram, B. N., Mareš, J., Martínez Yerena, J. A., Hrouzek, P., Sobotka, R. et al. 2021. Hunting the eagle killer: A cyanobacterial neurotoxin causes vacuolar myelinopathy. Science 371:1335.

Brito, A., Ramos, V., Mota, R., Lima, S., Santos, A., Vieira, J., Vieira, C. P., Kaštovský, J., Vasconcelos, V. M. & Tamagnini, P. 2017. Description of new genera and species of marine cyanobacteria from the Portuguese Atlantic coast. Mol. Phylogenet. Evol. 111:18-34.

Brito, A., Ramos, V., Seabra, R., Santos, A., Santos, C. L., Lopo, M., Ferreira, S. et al. 2012. Culture-dependent characterization of cyanobacterial diversity in the intertidal zones of the Portuguese coast: a polyphasic study. Syst. Appl. Microbiol. 35:110-9.

Brown, I., Mummey, D. & Cooksey, K. E. 2005. A novel cyanobacterium exhibiting an elevated tolerance for iron. FEMS Microbiol. Ecol. 52:307-14.

Buch, B., Martins, M. D. & Branco, L. H. Z. 2017. A widespread cyanobacterium supported by polyphasic approach: proposition of Koinonema pervagatum gen. & sp. nov. (Oscillatoriales). J. Phycol. 53:1097-105.

Burger-Wiersma, T., Stal, L. J. & Mur, L. R. 1989. Prochlorthrix hollandica gen. nov. sp. nov., a filamentous oxygenic photoautotrophic procaryote containing chlorophylls a and b: assignment to Prochlorotrichaceae fam. nov. and order Prochlorales Florenzano, Balloni, and Materassi 1986, with emendation of the ordinal description. Int. J. Syst. Evol. Microbiol. 39:250-7.

Burgsdorf, I., Slaby, B. M., Handley, K. M., Haber, M., Blom, J., Marshall, C. W., Gilbert, J. A., Hentschel, U. & Steindler, L. 2015. Lifestyle evolution in cyanobacterial symbionts of sponges. MBio 6:e00391-15.

Cai, F. & Li, R. 2020. Purpureonostoc, a new name for a recently described genus of Nostoc-like cyanobacteria. Fottea 20:111-1.

Cai, F., Li, X., Geng, R., Peng, X. & Li, R. 2019a. Phylogenetically distant clade of Nostoc-like taxa with the description of Minunostoc gen. nov. and Minunostoc cylindricum sp. nov. Fottea 19:13-24.

Cai, F., Li, X., Yang, Y., Jia, N., Huo, D. & Li, R. 2019b. Compactonostoc shennongjiaensis gen. & sp. nov. (Nostocales, Cyanobacteria) from a wet rocky wall in China. Phycologia 58:200-10.

Caroppo, C., Albertano, P., Bruno, L., Montinari, M., Rizzi, M., Vigliotta, G. & Pagliara, P. 2012. Identification and characterization of a new Halomicronema species (Cyanobacteria) isolated from the Mediterranean marine sponge Petrosia ficiformis (Porifera). Fottea 12:315-26.

Casamatta, D., Stanić, D., Gantar, M. & Richardson, L. L. 2012. Characterization of Roseofilum reptotaenium (Oscillatoriales, Cyanobacteria) gen. et sp. nov. isolated from Caribbean black band disease. Phycologia 51:489-99.

Casamatta, D. A., Villanueva, C. D., Garvey, A. D., Stocks, H. S., Vaccarino, M., Dvořák, P., Hašler, P. & Johansen, J. R. 2020. Reptodigitus chapmanii (Nostocales, Hapalosiphonaceae) gen. nov.: A unique nostocalean (Cyanobacteria) genus based on a polyphasic approach. J. Phycol. 56:425-36.

Castenholz, R. W., Wilmotte, A., Herdman, M., Rippka, R., Waterbury, J. B., Iteman, I. & Hoffmann, L. 2001. Phylum BX. Cyanobacteria. In Boone, D. R., Castenholz, R. W. & Garrity, G. M. [Eds.] Bergey's Manual of Systematic Bacteriology: Volume One: The Archaea and the Deeply Branching and Phototrophic Bacteria. Springer New York, New York, NY, pp. 473-599.

Cellamare, M., Duval, C., Drelin, Y., Djediat, C., Touibi, N., Agogue, H., Leboulanger, C., Ader, M. & Bernard, C. 2018. Characterization of phototrophic microorganisms and description of new cyanobacteria isolated from the saline-alkaline crater-lake Dziani Dzaha (Mayotte, Indian Ocean). FEMS Microbiol. Ecol. 94:fiy108.

Chakraborty, S., Maruthanayagam, V., Achari, A., Pramanik, A., Jaisankar, P. & Mukherjee, J. 2019. Euryhalinema mangrovii gen. nov., sp. nov. and Leptoelongatus litoralis gen. nov., sp. nov. (Leptolyngbyaceae) isolated from an Indian mangrove forest. Phytotaxa 422:58-74.

Chatchawan, T., Komárek, J., Strunecký, O., Šmarda, J. & Peerapornpisal, Y. 2012. Oxynema, a new genus separated from the genus Phormidium (Cyanophyta). Cryptogam. Algol. 33:41-59.

Chen, M., Floetenmeyer, M. & Bibby, T. S. 2009. Supramolecular organization of phycobiliproteins in the chlorophyll d-containing cyanobacterium Acaryochloris marina. FEBS Lett. 583:2535-9.

Choi, D. H., Noh, J. H., Lee, C. M. & Rho, S. 2008. Rubidibacter lacunae gen. nov., sp nov., a unicellular, phycoerythrin-containing cyanobacterium isolated from seawater of Chuuk lagoon, Micronesia. Int. J. Syst. Evol. Microbiol. 58:2807-11.

Costa, J. L., Romero, E. M. & Lindblad, P. 2004. Sequence based data supports a single Nostoc strain in individual coralloid roots of cycads. FEMS Microbiol. Ecol. 49:481-7.

Cumbers, J. & Rothschild, L. J. 2014. Salt tolerance and polyphyly in the cyanobacterium Chroococcidiopsis (Pleurocapsales). J. Phycol. 50:472-82.

da Silva Malone, C. F., Rigonato, J., Laughinghouse, H. D., Schmidt, E. C., Bouzon, Z. L., Wilmotte, A., Fiore, M. F. & Sant'Anna, C. L. 2015. Cephalothrix gen. nov. (Cyanobacteria): towards an intraspecific phylogenetic evaluation by multilocus analyses. Int. J. Syst. Evol. Microbiol. 65:2993-3007.

Dadheech, P. K., Abed, R. M. M., Mahmoud, H., Mohan, M. K. & Krienitz, L. 2012. Polyphasic characterization of cyanobacteria isolated from desert crusts, and the description of Desertifilum tharense gen. et sp. nov. (Oscillatoriales). Phycologia 51:260-70.

Darriba, D., Taboada, G. L., Doallo, R. & Posada, D. 2012. jModelTest 2: more models, new heuristics and parallel computing. Nat. Methods 9:772.

De Wever, A., Benzerara, K., Coutaud, M., Caumes, G., Poinsot, M., Skouri-Panet, F., Laurent, T., Duprat, E. & Gugger, M. 2019. Evidence of high Ca uptake by cyanobacteria forming intracellular CaCO3 and impact on their growth. Geobiology 17:676-90.

Desikachary, T. V. 1959. Cyanophyta. Indian Council of Agricultural Research, New Delhi, 686 pp.

Di Rienzi, S. C., Sharon, I., Wrighton, K. C., Koren, O., Hug, L. A., Thomas, B. C., Goodrich, J. K., Bell, J. T., Spector, T. D., Banfield, J. F. & Ley, R. E. 2013. The human gut and groundwater harbor non-photosynthetic bacteria belonging to a new candidate phylum sibling to Cyanobacteria. eLife 2:e01102.

Drouet, F. 1968. Revision of the Classification of the Oscillatoriaceae. Museum of Natural History, London, 370 pp.

Dvořák, P., Casamatta, D. A., Poulíčková, A., Hašler, P., Ondřej, V. & Sanges, R. 2014a. Synechococcus: 3 billion years of global dominance. Mol. Ecol. 23:5538-51.

Dvořák, P., Hašler, P., Pitelková, P., Tabáková, P., Casamatta, D. A. & Poulíčková, A. 2017. A new cyanobacterium from the Everglades, Florida - Chamaethrix gen. nov. Fottea 17:269-76.

Dvořák, P., Hindák, F., Hašler, P., Hindáková, A. & Poulíčková, A. 2014b. Morphological and molecular studies of Neosynechococcus sphagnicola, gen. et sp. nov. (Cyanobacteria, Synechococcales). Phytotaxa 170:24-34.

Elenkin, A. A. 1938. Monographia algarum cyanophycearum aquidulcium et terrestrium in finibus USSR inventarum. Izd. AN SSSR, Moscow-Leningrad, 1908 pp.

Engene, N., Rottacker, E. C., Kaštovský, J., Byrum, T., Choi, H., Ellisman, M. H., Komárek, J. & Gerwick, W. H. 2012. Moorea producens gen. nov., sp. nov. and Moorea bouillonii comb. nov., tropical marine cyanobacteria rich in bioactive secondary metabolites. Int. J. Syst. Evol. Microbiol. 62:1171-8.

Engene, N., Tronholm, A., Salvador-Reyes, L. A., Luesch, H. & Paul, V. J. 2015. Caldora penicillata gen. nov., comb. nov. (cyanobacteria), a pantropical marine species with biomedical relevance. J. Phycol. 51:670-81.

Fewer, D., Friedl, T. & Büdel, B. 2002. Chroococcidiopsis and heterocyst-differentiating cyanobacteria are each other's closest living relatives. Mol. Phylogenet. Evol. 23:82-90.

Foster, J. S., Green, S. J., Ahrendt, S. R., Golubić, S., Reid, R. P., Hetherington, K. L. & Bebout, L. 2009. Molecular and morphological characterization of cyanobacterial diversity in the stromatolites of Highborne Cay, Bahamas. ISME J. 3:573-87.

Galica, T., Borbone, N., Mareš, J., Kust, A., Caso, A., Esposito, G., Saurav, K. et al. 2021. Cyanochelins, an overlooked class of widely distributed cyanobacterial siderophores, discovered by silent gene cluster awakening. Appl. Environ. Microbiol. 87:e0312820.

Gama, W. A., Rigonato, J., Fiore, M. F. & Sant'Anna, C. L. 2019. New insights into Chroococcus (Cyanobacteria) and two related genera: Cryptococcum gen. nov. and Inacoccus gen. nov. Eur. J. Phycol. 54:315-25.

Gao, Z. M., Wang, Y., Tian, R. M., Wong, Y. H., Batang, Z. B., Al-Suwailem, A. M., Bajic, V. B. & Qian, P. Y. 2014. Symbiotic adaptation drives genome streamlining of the cyanobacterial sponge symbiont "Candidatus Synechococcus spongiarum". MBio 5:e00079-14.

Garcia-Pichel, F., Nübel, U. & Muyzer, G. 1998. The phylogeny of unicellular, extremely halotolerant cyanobacteria. Arch. Microbiol. 169:469-82.

Geitler, L. 1925. Cyanophyceae. Gustav Fischer, Jena, 450 pp.

Geitler, L. 1932. Cyanophyceae. In Rabenhorst's Kryptogamenflora von Deutschland, Österreich und der Schweiz, Band XIV (reprint 1985). Koenigstein, Koeltz, p. 1196.

Geitler, L. 1942. Schizophyta (Klasse Schizophyceae). In Engler, A. & Prantl, K. [Eds.] Natürliche Pflanzenfamilien 1b. Duncker & Humblot, Berlin, pp. 1-232.

Genuário, D. B., de Souza, W. R., Monteiro, R. T. R., Sant'Anna, C. L. & Melo, I. S. 2018. Amazoninema gen. nov., (Synechococcales, Pseudanabaenaceae) a novel cyanobacteria genus from Brazilian Amazonian rivers. Int. J. Syst. Evol. Microbiol. 68:2249-57.

Genuário, D. B., Vaz, M., Hentschke, G. S., Sant'Anna, C. L. & Fiore, M. F. 2015. Halotia gen. nov., a phylogenetically and physiologically coherent cyanobacterial genus isolated from marine coastal environments. Int. J. Syst. Evol. Microbiol. 65:663-75.

Gómez, R., Erpenbeck, D., Richelle-Maurer, E., Van Dijk, T., Woldringh, C. & Van Soest, R. 2004. Affinities of the Aphanocapsa feldmanni-like cyanobacteria from the marine sponge Xestospongia muta based on genetic and morphological analysis. Bollettino dei musei e degli istituti biologici dell' Universita di Genova 68:335-47.

Gómez-Lojero, C., Leyva-Castillo, L. E., Herrera-Salgado, P., Barrera-Rojas, J., Ríos-Castro, E. & Gutiérrez-Cirlos, E. B. 2018. Leptolyngbya CCM 4, a cyanobacterium with far-red photoacclimation from Cuatro Ciénegas Basin, México. Photosynthetica 56:342-53.

Gomont, M. 1892. Monographie des Oscillariées (Nostocacées homocystées). Annales des Sciences Naturelles Botanique Séries 7:263-368.

Grettenberger, C. L., Sumner, D. Y., Wall, K., Brown, C. T., Eisen, J. A., Mackey, T. J., Hawes, I., Jospin, G. & Jungblut, A. D. 2020. A phylogenetically novel cyanobacterium most closely related to Gloeobacter. ISME J. 14:2142-52.

Gromov, B. V. & Mamkaeva, K. A. 1980. New genus of bacteria, Vampirovibrio, parasitizing chlorella and previously assigned to the genus Bdellovibrio. Mikrobiologiia 49:165-7.

Gugger, M. F. & Hoffmann, L. 2004. Polyphyly of true branching cyanobacteria (Stigonematales). Int. J. Syst. Evol. Microbiol. 54:349-57.

Guglielmi, G. & Cohen-Bazire, G. 1984. Étude taxonomique d'un genre de cyanobactérie oscillatoriacée: Le genre Pseudanabaena Lauterborn. I. Étude ultrastructurale. Protistologica 20:377-91.

Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser. 41:95-8.

Harke, M. J., Steffen, M. M., Gobler, C. J., Otten, T. G., Wilhelm, S. W., Wood, S. A. & Paerl, H. W. 2016. A review of the global ecology, genomics, and biogeography of the toxic cyanobacterium, Microcystis spp. Harmful Algae 54:4-20.

Hašler, P., Casamatta, D., Dvořák, P. & Poulíčková, A. 2019. Jacksonvillea apiculata (Oscillatoriales, Cyanobacteria) gen. & sp. nov.: A new genus of filamentous, epipsamic cyanobacteria from North Florida. Phycologia 56:284-95.

Hašler, P. & Dvořák, P. 2014. A new genus of filamentous epipelic cyanobacteria, Johansenia. Preslia 86:91-4.

Hašler, P. & Poulíčková, A. 2010. Diversity, taxonomy and autecology of autochtonous epipelic cyanobacteria of the genus Komvophoron (Borziaceae, Oscillatoriales): a study on populations from The Czech Republic and British Isles. Biologia 65:7-16.

Hauer, T., Bohunická, M., Johansen, J. R., Mareš, J. & Berrendero-Gómez, E. 2014. Reassessment of the cyanobacterial family Microchaetaceae and establishment of new families Tolypothrichaceae and Godleyaceae. J. Phycol. 50:1089-100.

Heidari, F., Zima, J., Riahi, H. & Hauer, T. 2018. New simple trichal cyanobacterial taxa isolated from radioactive thermal springs. Fottea 18:137-49.

Hentschke, G. S., Johansen, J. R., Pietrasiak, N., Fiore, M. D. F., Rigonato, J., Sant’ Anna, C. L. & Komárek, J. 2016. Phylogenetic placement of Dapisostemon gen. nov. and Streptostemon, two tropical heterocytous genera (Cyanobacteria). Phytotaxa 245:129-143.

Hentschke, S. G., Johansen, J. R., Pietrasiak, N., Rigonato, J., Fiore, M. F. & Sant'Anna, C. L. 2017. Komarekiella atlantica gen. et sp. nov. (Nostocaceae, Cyanobacteria): a new subaerial taxon from the Atlantic Rainforest and Kauai, Hawaii. Fottea 17:178-90.

Ho, M. Y., Gan, F., Shen, G., Zhao, C. & Bryant, D. A. 2017. Far-red light photoacclimation (FaRLiP) in Synechococcus sp. PCC 7335: I. Regulation of FaRLiP gene expression. Photosynth. Res. 131:173-86.

Hoffmann, L., Kaštovský, J. & Komárek, J. 2005. System of cyanoprokaryotes (Cyanobacteria) - state in 2004. Algol. Stud. / Arch. Hydrobiol. Suppl. 117:95-115.

Hrouzek, P., Lukešová, A., Mareš, J. & Ventura, S. 2013. Description of the cyanobacterial genus Desmonostoc gen. nov. including D. muscorum comb. nov. as a distinct, phylogenetically coherent taxon related to the genus Nostoc. Fottea 13:201-13.

Jahodářová, E., Dvořák, P., Hašler, P. & Poulíčková, A. 2017. Revealing hidden diversity among tropical cyanobacteria: the new genus Onodrimia (Synechococcales, Cyanobacteria) described using the polyphasic approach. Phytotaxa 326:28-40.

Johansen, J. R. & Casamatta, D. A. 2005. Recognizing cyanobacterial diversity through adoption of a new species paradigm. Algol. Stud. Arch. Hydrobiol. Suppl. 117:71-93.

Johansen, J. R., González-Resendiz, L., Escobar-Sánchez, V., Segal-Kischinevzky, C., Martínez-Yerena, J. A., Hernández-Sánchez, J., Hernández-Pérez, G. & León-Tejera, H. 2021. When will taxonomic saturation be achieved? A case study in Nunduva and Kyrtuthrix (Rivulariaceae, Cyanobacteria). J. Phycol. 57:1699-720.

Jung, P., Azua-Bustos, A., Gonzalez-Silva, C., Mikhailyuk, T., Zabicki, D., Holzinger, A., Lakatos, M. & Büdel, B. 2021a. Emendation of the coccoid cyanobacterial genus Gloeocapsopsis and description of the new species Gloeocapsopsis diffluens sp. nov. and Gloeocapsopsis dulcis sp. nov. isolated from the coastal range of the Atacama Desert (Chile). Front. Microbiol. 12:671742.

Jung, P., D'Agostino, P. M., Brust, K., Büdel, B. & Lakatos, M. 2021b. Final destination? Pinpointing Hyella disjuncta sp. nov. PCC 6712 (Cyanobacteria) based on taxonomic aspects, multicellularity, nitrogen fixation and biosynthetic gene clusters. Life 11:916.

Jung, P., Mikhailyuk, T., Emrich, D., Baumann, K., Dultz, S. & Büdel, B. 2020. Shifting boundaries: ecological and geographical range extension based on three new species in the cyanobacterial genera Cyanocohniella, Oculatella, and Aliterella. J. Phycol. 56:1216-31.

Jutersek, M., Klemencic, M. & Dolinar, M. 2017. Discrimination between Synechocystis members (cyanobacteria) based on heterogeneity of their 16S rRNA and ITS regions. Acta Chim. Slov. 64:804-17.

Kaštovský, J., Berrendero-Gómez, E., Hladil, J. & Johansen, J. R. 2014. Cyanocohniella calida gen. et sp. nov. (Cyanobacteria: Aphanizomenonaceae) a new cyanobacterium from the thermal springs from Karlovy Vary. Czech Republic. Phytotaxa 181:279-92.

Katoh, K. & Standley, D. M. 2013. MAFFT Multiple Sequence Alignment Software Version 7: Improvements in performance and usability. Mol. Biol. Evol. 30:772-80.

Kerfeld, C. A. & Melnicki, M. R. 2016. Assembly, function and evolution of cyanobacterial carboxysomes. Curr. Opin. Plant Biol. 31:66-75.

Kilgore, C., Johansen, J. R., Mai, T., Hauer, T., Casamatta, D. A. & Sheil, C. A. 2018. Molecular characterization of Geitleria appalachiana sp. nov. (Nostocales, Cyanobacteria) and formation of Geitleriaceae fam. nov. Fottea 18:150-63.

Kling, H. J., Laughinghouse, D. H., IV, Šmarda, J., Komárek, J., Acreman, J., Bruun, K., Watson, S. B. & Chen, F. 2012. A new red colonial Pseudanabaena (Cyanoprokaryota, Oscillatoriales) from North American large lakes. Fottea 12:327-39.

Komárek, J. 2005. Phenotype diversity of the heterocytous cyanoprokaryotic genus Anabaenopsis. Czech Phycology 5:1-35.

Komárek, J. 2016. Review of the cyanobacterial genera implying planktic species after recent taxonomic revisions according to polyphasic methods: state as of 2014. Hydrobiologia 764:259-70.

Komárek, J. 2017. Cyanoprokaryota. Part 3: Heterocytous Genera. Springer Spektrum, Berlin, 1130 pp.

Komárek, J. 2020. Quo vadis, taxonomy of cyanobacteria (2019). Fottea 20:104-10.

Komárek, J. & Anagnostidis, K. 1998. Cyanoprokaryota 1. Teil: Chroococcales. Gustav Fischer, Jena-Stuttgart-Lübeck-Ulm, 548 pp.

Komárek, J. & Anagnostidis, K. 2005. Cyanoprokaryota 2. Teil: Oscillatoriales. Elsevier/Spektrum, Heidelberg, 759 pp.

Komárek, J. & Hindák, F. 1988. Taxonomy of the new isolated strains of Chroococcidiopsis. Algol. Stud./ Arch. Hydrobiol. Suppl. 46:311-29.

Komárek, J., Johansen, J. R., Šmarda, J. & Strunecký, O. 2020. Phylogeny and taxonomy of Synechococcus-like cyanobacteria. Fottea 20:171-91.

Komárek, J., Kaštovský, J., Mareš, J. & Johansen, J. R. 2014. Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach. Preslia 86:295-335.

Komárek, J. & Lukavský, J. 1988. Arthronema, a new cyanophyte genus from Afro-Asian deserts. Algol. Stud. Arch. Hydrobiol. Suppl. 80:249-67.

Komárek, J. & Mareš, J. 2012. An update to modern taxonomy (2011) of freshwater planktic heterocytous cyanobacteria. Hydrobiologia 698:327-51.

Komárková, J. & Šimek, K. 2003. Unicellular and colonial formations of picoplanktonic cyanobacteria under variable environmental conditions and predation pressure. Algol. Stud. Arch. Hydrobiol. Suppl. 109:327-40.

Komárková, J., Zapomělová, E. & Komárek, J. 2013. Chakia (cyanobacteria), a new heterocytous genus from Belizean marshes identified on the basis of the 16S rRNA gene. Fottea 13:227-33.

Konstantinou, D., Voultsiadou, E., Panteris, E. & Gkelis, S. 2021. Revealing new sponge-associated cyanobacterial diversity: Novel genera and species. Mol. Phylogenet. Evol. 155:106991.

Konstantinou, D., Voultsiadou, E., Panteris, E., Zervou, S. K., Hiskia, A. & Gkelis, S. 2019. Leptothoe, a new genus of marine cyanobacteria (Synechococcales) and three new species associated with sponges from the Aegean Sea. J. Phycol. 55:882-97.

Korelusová, J., Kaštovský, J. & Komárek, J. 2009. Heterogeneity of the cyanobacterial genus Synechocystis and description of a new genus. Geminocystis. J. Phycol. 45:928-37.

Kozlíková-Zapomělová, E., Chatchawan, T., Kaštovský, J. & Komárek, J. 2016. Phylogenetic and taxonomic position of the genus Wollea with the description of Wollea salina sp. nov. (Cyanobacteria, Nostocales). Fottea 16:43-55.

Kurmayer, R., Christiansen, G., Holzinger, A. & Rott, E. 2018. Single colony genetic analysis of epilithic stream algae of the genus Chamaesiphon spp. Hydrobiologia 811:61-75.

Lamparter, T., Babian, J., Frohlich, K., Mielke, M., Weber, N., Wunsch, N., Zais, F., Schulz, K., Aschmann, V., Spohrer, N. & Krauss, N. 2022. The involvement of type IV pili and the phytochrome CphA in gliding motility, lateral motility and photophobotaxis of the cyanobacterium Phormidium lacuna. PLoS ONE 17:e0249509.

Lamprinou, V., Christodoulou, M., Hernández-Mariné, M., Parmakelis, A. & Economou-Amilli, A. 2016. Spelaeonaias gen. nov., a new true-branched cyanobacterium from Cave Vlychada (Diros, Peloponnese, Greece). Phytotaxa 282:171-85.

Lauterborn, R. 1915. Die sapropelische Lebewelt. Ein Beitrag zur Biologie des Faulschlammes natürlicher Gewässer. Verhandlungen des Naturhistorisch-Medizinischen Vereins zu Heidelberg 2:395-481.

León-Tejera, H. P., González-Resendiz, L., Johansen, J. R., Segal-Kischinevsky, C., Escobar, V. & Alba Lois, L. 2016. Phylogenetic position reevaluation of Kyrtuthrix and description of a new species K. huatulcensis from Mexico's Pacific coast. Phytotaxa 278:1-18.

Lewin, R. A. 1977. Prochloron, type genus of the Prochlorophyta. Phycologia 16:217-7.

Liang, Y., Kaczmarek, M. B., Kasprzak, A. K., Tang, J., Shah, M. M. R., Jin, P., Klepacz-Smółka, A., Cheng, J. J., Ledakowicz, S. & Daroch, M. 2018. Thermosynechococcaceae as a source of thermostable C-phycocyanins: properties and molecular insights. Algal Res. 35:223-35.

Lien, T. T. N., Cronberg, G., Moestrup, O. & Daugbjerg, N. 2013. Annamia toxica gen. et sp nov (Cyanobacteria), a freshwater cyanobacterium from Vietnam that produces microcystins: ultrastructure, toxicity and molecular phylogenetics. Phycologia 52:25-36.

Linhartová, M., Skotnicová, P., Hakkila, K., Tichý, M., Komenda, J., Knoppová, J., Gilabert, J. F., Guallar, V., Tyystjarvi, T. & Sobotka, R. 2021. Mutations suppressing the lack of prepilin peptidase provide insights into the maturation of the major pilin protein in cyanobacteria. Front. Microbiol. 12:756912.

Lücking, R., Barrie, F. R. & Genney, D. 2014. Dictyonema coppinsii, a new name for the European species known as Dictyonema interruptum (Basidiomycota: Agaricales: Hygrophoraceae), with a validation of its photobiont Rhizonema (Cyanoprokaryota: Nostocales: Rhizonemataceae). Lichenol. 46:261-7.

Machado-De-Lima, N. M. & Branco, L. H. Z. 2020. Biological soil crusts: new genera and species of Cyanobacteria from Brazilian semi-arid regions. Phytotaxa 470:263-81.

Mai, T., Johansen, J. R., Pietrasiak, N., Bohunická, M. & Martin, M. P. 2018. Revision of the Synechococcales (Cyanobacteria) through recognition of four families including Oculatellaceae fam. nov. and Trichocoleaceae fam. nov. and six new genera containing 14 species. Phytotaxa 365:1-59.

Mareš, J. 2018. Multilocus and SSU rRNA gene phylogenetic analyses of available cyanobacterial genomes, and their relation to the current taxonomic system. Hydrobiologia 811:19-34.

Mareš, J., Hrouzek, P., Kaňa, R., Ventura, S., Strunecký, O. & Komárek, J. 2013. The primitive thylakoid-less cyanobacterium Gloeobacter is a common rock-dwelling organism. PLoS ONE 8:e66323.

Mareš, J., Johansen, J. R., Hauer, T., Zima, J., Jr., Ventura, S., Cuzman, O., Tiribilli, B. & Kaštovský, J. 2019b. Taxonomic resolution of the genus Cyanothece (Chroococcales, Cyanobacteria), with a treatment on Gloeothece and three new genera, Crocosphaera, Rippkae, and Zehria. J. Phycol. 55:578-610.

Mareš, J., Lara, Y., Dadáková, I., Hauer, T., Uher, B., Wilmotte, A. & Kaštovský, J. 2015. Phylogenetic analysis of cultivation-resistant terrestrial cyanobacteria with massive sheaths (Stigonema spp. and Petalonema alatum, Nostocales, Cyanobacteria) using single-cell and filament sequencing of environmental samples. J. Phycol. 51:288-97.

Mareš, J., Strunecký, O., Bučinská, L. & Wiedermannová, J. 2019a. Evolutionary patterns of thylakoid architecture in cyanobacteria. Front. Microbiol. 10:277.

Margheri, M. C., Ventura, S., Kaštovský, J. & Komárek, J. 2008. The taxonomic validation of the cyanobacterial genus Halothece. Phycologia 47:477-86.

Marquardt, J., Morschel, E., Rhiel, E. & Westermann, M. 2000. Ultrastructure of Acaryochloris marina, an oxyphotobacterium containing mainly chlorophyll d. Arch. Hydrobiol. 174:181-8.

Martins, M. D., Rigonato, J., Taboga, S. R. & Branco, L. H. Z. 2016. Proposal of Ancylothrix gen. nov., a new genus of Phormidiaceae (Cyanobacteria, Oscillatoriales) based on a polyphasic approach. Int. J. Syst. Evol. Microbiol. 66:2396-405.

Mata, T. M., Martins, A. A. & Caetano, N. S. 2010. Microalgae for biodiesel production and other applications: A review. Renew. Sustain. Energy Rev. 14:217-32.

McGregor, G. B. & Sendall, B. C. 2013. Komvophoron kgarii sp. nov. (Oscillatoriales), a new epipelic cyanobacterium from subtropical eastern Australia. Phycologia 52:472-80.

McGregor, G. B. & Sendall, B. C. 2015. Phylogeny and toxicology of Lyngbya wollei (Cyanobacteria, Oscillatoriales) from north-eastern Australia, with a description of Microseira gen. nov. J. Phycol. 51:109-19.

McGregor, G. B. & Sendall, B. C. 2019. Potamosiphon australiensis gen. nov. sp. nov. (Oscillatoriales), a new filamentous cyanobacterium from subtropical north-eastern Australia. Phytotaxa 387:77-93.

Meffert, M. E. 1988. Limnothrix Meffert nov. gen. The unsheathed planktic cyanophycean filaments with polar and central gas vacuoles. Algol. Stud. Arch. Hydrobiol. Suppl. 50-53:269-76.

Minh, B. Q., Schmidt, H. A., Chernomor, O., Schrempf, D., Woodhams, M. D., von Haeseler, A. & Lanfear, R. 2020. IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Mol. Biol. Evol. 37:1530-4.

Miscoe, L. H., Johansen, J., Vaccarino, M. A., Pietrasiak, N. & Sherwood, A. R. 2016. The diatom flora and cyanobacteria from caves on Kauai, Hawaii. II. Novel cyanobacteria from caves on Kauai, Hawaii. Bibl. Phycol. 120:1-152.

Miyashita, H., Ikemoto, H., Kurano, N., Miyachi, S. & Chihara, M. 2003. Acaryochloris marina gen. et sp. nov. (Cyanobacteria), an oxygenic photosynthetic prokaryote containing chl d as a major pigment. J. Phycol. 39:1247-53.

Moreira, D., Tavera, R., Benzerara, K., Skouri-Panet, F., Couradeau, E., Gerard, E., Fonta, C. L., Novelo, E., Zivanovic, Y. & Lopez-Garcia, P. 2017. Description of Gloeomargarita lithophora gen. nov., sp. nov., a thylakoid-bearing, basal-branching cyanobacterium with intracellular carbonates, and proposal for Gloeomargaritales ord. nov. Int. J. Syst. Evol. Microbiol. 67:653-8.

Mühlsteinová, R., Hauer, T., De Ley, P. & Pietrasiak, N. 2018. Seeking the true Oscillatoria: a quest for a reliable phylogenetic and taxonomic reference point. Preslia 90:151-69.

Mühlsteinová, R., Johansen, J. R., Pietrasiak, N., Martin, M. P., Osorio-Santos, K. & Warren, S. D. 2014. Polyphasic characterization of Trichocoleus desertorum sp. nov. (Pseudanabaenales, Cyanobacteria) from desert soils and phylogenetic placement of the genus Trichocoleus. Phytotaxa 163:241-61.

Nakayama, T., Nomura, M., Takano, Y., Tanifuji, G., Shiba, K., Inaba, K., Inagaki, Y. & Kawata, M. 2019. Single-cell genomics unveiled a cryptic cyanobacterial lineage with a worldwide distribution hidden by a dinoflagellate host. Proc. Natl. Acad. Sci. USA 116:15973-8.

Niiyama, Y., Tuji, A. & Tsujimura, S. 2011. Umezakia natans M.Watan. does not belong to Stigonemataceae but to Nostocaceae. Fottea 11:163-9.

Ohkubo, S., Miyashita, H., Murakami, A., Takeyama, H., Tsuchiya, T. & Mimuro, M. 2006. Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae. Appl. Environ. Microbiol. 72:7912-5.

Oren, A., Mareš, J. & Rippka, R. 2022. Validation of the names Cyanobacterium and Cyanobacterium stanieri, and proposal of Cyanobacteriota phyl. nov. Int. J. Syst. Evol. Microbiol. 72:005528.

Osorio-Santos, K., Pietrasiak, N., Bohunická, M., Miscoe, L. H., Kováčik, L., Martin, M. P. & Johansen, J. R. 2014. Seven new species of Oculatella (Pseudanabaenales, Cyanobacteria): taxonomically recognizing cryptic diversification. Eur. J. Phycol. 49:450-70.

Parks, D. H., Chuvochina, M., Chaumeil, P.A., Rinke, C., Mussig, A. J. & Hugenholtz, P. 2020. A complete domain-to-species taxonomy for Bacteria and Archaea. Nat. Biotechnol. 38:1079-86.

Parks, D. H., Chuvochina, M., Waite, D. W., Rinke, C., Skarshewski, A., Chaumeil, P. A. & Hugenholtz, P. 2018. A standardized bacterial taxonomy based on genome phylogeny substantially revises the tree of life. Nat. Biotechnol. 36:996-1004.

Partensky, F., Six, C., Ratin, M., Garczarek, L., Vaulot, D., Probert, I., Calteau, A. et al. 2018. A novel species of the marine cyanobacterium Acaryochloris with a unique pigment content and lifestyle. Sci. Rep. 8:9142.

Perkerson, R. B., Johansen, J. R., Kováčik, L., Brand, J., Kaštovský, J. & Casamatta, D. A. 2011. A unique Pseudanabaenalean (cyanobacteria) genus Nodosilinea gen. nov. based on morphological and molecular data. J. Phycol. 47:1397-412.

Pietrasiak, N., Osorio-Santos, K., Shalygin, S., Martin, M. P. & Johansen, J. R. 2019. When is a lineage a species? A case study in Myxacorys gen. nov. (Synechococcales: Cyanobacteria) with the description of two new species from the Americas. J. Phycol. 55:976-96.

Porta, D., Hernández-Mariné, M., Herdman, M. & Rippka, R. 2003. Structural and ultrastructural characterization of Symploca atlantica Gomont, strain PCC 8002 (Oscillatoriales, Cyanophyta, Cyanobacteria). Algol. Stud. Arch. Hydrobiol. Suppl. 109:509-24.

Raabová, L., Kováčik, L., Elster, J. & Strunecký, O. 2019. Review of the genus Phormidesmis (Cyanobacteria) based on environmental, morphological, and molecular data with description of a new genus Leptodesmis. Phytotaxa 395:1-16.

Ragon, M., Benzerara, K., Moreira, D., Tavera, R. & Lopez-Garcia, P. 2014. 16 S rDNA-based analysis reveals cosmopolitan occurrence but limited diversity of two cyanobacterial lineages with contrasted patterns of intracellular carbonate mineralization. Front. Microbiol. 5:331.

Rahmatpour, N., Hauser, D. A., Nelson, J. M., Chen, P. Y., Villarreal, A. J., Ho, M. Y. & Li, F. W. 2021. A novel thylakoid-less isolate fills a billion-year gap in the evolution of Cyanobacteria. Curr. Biol. 31:2857-67.e4.

Rajaniemi-Wacklin, P., Rantala, A., Mugnai, M. A., Turicchia, S., Ventura, S., Komárková, J., Lepisto, L. & Sivonen, K. 2006. Correspondence between phylogeny and morphology of Snowella spp. and Woronichinia naegeliana, cyanobacteria commonly occurring in lakes. J. Phycol. 42:226-32.

Raven, J. A. & Sánchez-Baracaldo, P. 2021. Gloeobacter and the implications of a freshwater origin of Cyanobacteria. Phycologia 60:402-18.

Řeháková, K., Johansen, J. R., Casamatta, D. A., Xuesong, L. & Vincent, J. 2007. Morphological and molecular characterization of selected desert soil cyanobacteria: three species new to science including Mojavia pulchra gen. et sp. nov. Phycologia 46:481-502.

Řeháková, K., Mareš, J., Lukešová, A., Zapomělová, E., Bernardová, K. & Hrouzek, P. 2014. Nodularia (Cyanobacteria, Nostocaceae): a phylogenetically uniform genus with variable phenotypes. Phytotaxa 172:235-46.

Rexroth, S., Mullineaux, C. W., Ellinger, D., Sendtko, E., Rogner, M. & Koenig, F. 2011. The plasma membrane of the cyanobacterium Gloeobacter violaceus contains segregated bioenergetic domains. Plant Cell 23:2379-90.

Rigonato, J., Gama, W. A., Alvarenga, D. O., Branco, L. H. Z., Brandini, F. P., Genuário, D. B. & Fiore, M. F. 2016. Aliterella atlantica gen. nov., sp. nov., and Aliterella Antarctica sp. nov., novel members of coccoid Cyanobacteria. Int. J. Syst. Evol. Microbiol. 66:2853-61.

Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M. & Stanier, R. Y. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111:1-61.

Rippka, R., Waterbury, J. & Cohen-Bazire, G. 1974. Cyanobacterium which lacks thylakoids. Arch. Microbiol. 100:419-36.

Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D. L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M. A. & Huelsenbeck, J. P. 2012. MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61:539-42.

Saber, A. A., Cantonati, M., Mareš, J., Anesi, A. & Guella, G. 2019. Polyphasic characterization of Westiellopsis prolifica (Hapalosiphonaceae, Cyanobacteria) from the El-Farafra Oasis (Western Desert, Egypt). Phycologia 56:697-709.

Salazar, V. W., Tschoeke, D. A., Swings, J., Cosenza, C. A., Mattoso, M., Thompson, C. C. & Thompson, F. L. 2020. A new genomic taxonomy system for the Synechococcus collective. Environ. Microbiol. 22:4557-70.

Salmaso, N., Cerasino, L., Boscaini, A. & Capelli, C. 2016. Planktic Tychonema (Cyanobacteria) in the large lakes south of the Alps: phylogenetic assessment and toxigenic potential. FEMS Microbiol. Ecol. 92:fiw155.

Samylina, O. S., Sinetova, M. A., Kupriyanova, E. V., Starikov, A. Y., Sukhacheva, M. V., Dziuba, M. V. & Tourova, T. P. 2021. Ecology and biogeography of the 'marine Geitlerinema' cluster and a description of Sodalinema orleanskyi sp. nov., Sodalinema gerasimenkoae sp. nov., Sodalinema stali sp. nov. and Baaleninema simplex gen. et sp. nov. (Oscillatoriales, Cyanobacteria). FEMS Microbiol. Ecol. 97:fiab104.

Sant'Anna, C. L., Gama, W. A., Rigonato, J., Correa, G., Mesquita, M. C. B. & Marinho, M. M. 2019. Phylogenetic connection among close genera of Aphanizomenonaceae (Cyanobacteria): Amphiheterocytum gen. nov. Cylindrospermopsis and Sphaerospermopsis. Alg. Res. 37:205-14.

Sant'Anna, C. L., Gama, W. A., Silva, T. G. & Vieira, A. A. H. 2018. A new species of Trichodesmium (Cyanobacteria) from freshwaters, Brazil. Rodriguésia 69:2019-24.

Saraf, A., Suradkar, A., Dawda, H. G., Gaysina, L. A., Gabidullin, Y., Kumat, A., Behere, I., Kotulkar, M., Batule, P. & Singh, P. 2021. Phylogenetic complexities of the members of Rivulariaceae with the re-creation of the family Calotrichaceae and description of Dulcicalothrix necridiiformans gen nov., sp nov., and reclassification of Calothrix desertica. FEMS Microbiol. Lett. 366:fnz219.

Saw, J. H., Schatz, M., Brown, M. V., Kunkel, D. D., Foster, J. S., Shick, H., Christensen, S., Hou, S., Wan, X. & Donachie, S. P. 2013. Cultivation and complete genome sequencing of Gloeobacter kilaueensis sp. nov., from a lava cave in Kilauea Caldera, Hawai'i. PLoS ONE 8:e76376.

Schaffner, J. H. 1922. The classification of plants. XII. Ohio J. Sci. 22:39.

Schirrmeister, B. E., de Vos, J. M., Antonelli, A. & Bagheri, H. C. 2013. Evolution of multicellularity coincided with increased diversification of cyanobacteria and the Great Oxidation Event. Proc. Natl. Acad. Sci. USA 110:1791-6.

Schuergers, N., Lenn, T., Kampmann, R., Meissner, M. V., Esteves, T., Temerinac-Ott, M., Korvink, J. G., Lowe, A. R., Mullineaux, C. W. & Wilde, A. 2016. Cyanobacteria use micro-optics to sense light direction. Elife 5:e12620.

Sciuto, K. & Moro, I. 2016. Detection of the new cosmopolitan genus Thermoleptolyngbya (Cyanobacteria, Leptolyngbyaceae) using the 16 S rRNA gene and 16 S-23 S ITS region. Mol. Phylogenet. Evol. 105:15-35.

Sciuto, K., Moschin, E. & Moro, I. 2017. Cryptic cyanobacterial diversity in the Giant Cave (Trieste, Italy): the new genus Timaviella (Leptolyngbyaceae). Cryptogamie Algol. 38:285-323.

Sendall, B. C. & McGregor, G. B. 2018. Cryptic diversity within the Scytonema complex: Characterization of the paralytic shellfish toxin producer Heterosyctonema crispum, and the establishment of the family Heteroscytonemataceae (Cyanobacteria/Nostocales). Harmful Algae 80:158-70.

Shalygin, S., Kavulic, K. J., Pietrasiak, N., Bohunická, M., Vaccarino, M. A., Chesarino, N. M. & Johansen, J. R. 2019. Neotypification of Pleurocapsa fuliginosa and epitypification of P. minor (Pleurocapsales): resolving a polyphyletic cyanobacterial genus. Phytotaxa 392:245-63.

Shalygin, S., Pietrasiak, N., Gomez, F., Mlewski, C., Gerard, E. & Johansen, J. R. 2018. Rivularia halophila sp. nov. (Nostocales, Cyanobacteria): the first species of Rivularia described with the modern polyphasic approach. Eur. J. Phycol. 53:537-48.

Shalygin, S., Shalygina, R., Johansen, J. R., Pietrasiak, N., Berrendero Gómez, E., Bohunická, M., Mareš, J. & Sheil, C. A. 2017. Cyanomargarita gen. nov. (Nostocales, Cyanobacteria): convergent evolution resulting in a cryptic genus. J. Phycol. 53:762-77.

Skuja, H. 1932. Algen. Botanische Ergebnisse der Deutschen Zentralasien-Expedition 1927-28. Repertorium Novarum Specierum Regni Vegetabilis 31:4-19.

Slaby, B. M. & Hentschel, U. 2017. Draft genome sequences of "Candidatus Synechococcus spongiarum," cyanobacterial symbionts of the mediterranean sponge Aplysina aerophoba. Genome Announc. 5:e00268-17.

Soares, F., Ramos, V., Trovão, J., Cardoso, S. M., Tiago, I. & Portugal, A. 2020. Parakomarekiella sesnandensis gen. et sp. nov. (Nostocales, Cyanobacteria) isolated from the Old Cathedral of Coimbra, Portugal (UNESCO World Heritage Site). Eur. J. Phycol. 56:301-15.

Song, G., Jiang, Y. & Li, R. 2015. Scytolyngbya timoleontis, gen. et sp. nov. (Leptolyngbyaceae, Cyanobacteria): a novel false branching Cyanobacteria from China. Phytotaxa 224:72-84.

Soo, R. M., Hemp, J. & Hugenholtz, P. 2019. Evolution of photosynthesis and aerobic respiration in the cyanobacteria. Free Radic. Biol. Med. 140:200-5.

Soo, R. M., Woodcroft, B. J., Parks, D. H., Tyson, G. W. & Hugenholtz, P. 2015. Back from the dead; the curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus. PeerJ 3:e968.

Stamatakis, A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312-3.

Starmach, K. 1966. Cyanophyta - sinice - Flora słodkowodna Polski 2. PAN -Panstw. Wyd, Nauk, Krakow, 860 pp.

Strunecký, O., Bohunická, M., Johansen, J. R., Čapková, K., Raabová, L., Dvořák, P. & Komárek, J. 2017. A revision of the genus Geitlerinema and a description of the genus Anagnostidinema gen. nov. (Oscillatoriophycidae, Cyanobacteria). Fottea 17:114-26.

Strunecký, O., Komárek, J., Johansen, J., Lukešová, A. & Elster, J. 2013. Molecular and morphological criteria for revision of the genus Microcoleus (Oscillatoriales, Cyanobacteria). J. Phycol. 49:1167-80.

Strunecký, O., Kopejtka, K., Goecke, F., Tomasch, J., Lukavský, J., Neori, A., Kahl, S., Pieper, D. H., Pilarski, P., Kaftan, D. & Koblížek, M. 2019. High diversity of thermophilic cyanobacteria in Rupite hot spring identified by microscopy, cultivation, single-cell PCR and amplicon sequencing. Extremophiles 23:35-48.

Strunecký, O., Raabová, L., Bernardová, A., Ivanova, A. P., Semanova, A., Crossley, J. & Kaftan, D. 2020. Diversity of cyanobacteria at the Alaska North Slope with description of two new genera: Gibliniella and Shackletoniella. FEMS. Microbiol. Ecol. 96:fiz189.

Strunecký, O., Wachtlová, M. & Koblížek, M. 2021. Whole genome phylogeny of Cyanobacteria documents a distinct evolutionary trajectory of marine picocyanobacteria. bioRxiv:2021.05.26.445609.

Suda, S., Watanabe, M. M., Otsuka, S., Mahakahant, A., Yongmanitchai, W., Nopartnaraporn, N., Liu, Y. & Day, J. G. 2002. Taxonomic revision of water-bloom-forming species of oscillatorioid cyanobacteria. Int. J. Syst. Evol. Microbiol. 52:1577-95.

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28:2731-9.

Tanabe, Y., Hodoki, Y., Sano, T., Tada, K. & Watanabe, M. M. 2018. Adaptation of the freshwater bloom-forming cyanobacterium Microcystis aeruginosa to brackish water is driven by recent horizontal transfer of sucrose genes. Front. Microbiol. 9:1150.

Thomazeau, S., Houdan-Fourmont, A., Couté, A., Duval, C., Couloux, A., Rousseau, F. & Bernard, C. 2010. The contribution of Sub-Saharan African strains to the phylogeny of Cyanobacteria: Focusing on the Nostocaceae (Nostocales, Cyanobacteria). J. Phycol. 46:564-79.

Thompson, A. W., Foster, R. A., Krupke, A., Carter, B. J., Musat, N., Vaulot, D., Kuypers, M. M. M. & Zehr, J. P. 2012. Unicellular cyanobacterium symbiotic with a single-celled eukaryotic alga. Science 337:1546-50.

Thu, N. K., Tanabe, Y., Matsuura, H. & Watanabe, M. M. 2020. Morphological, biochemical, and molecular characterization of Oscillatoria kawamurae (Oscillatoriales, Cyanobacteria) isolated from different geographical regions. Phycol. Res. 68:216-26.

Thu, N. K., Tanabe, Y., Yoshida, M., Matsuura, H. & Watanabe, M. M. 2012. Aerosakkonema funiforme gen. et sp. nov. (Oscillatoriales), a new gas-vacuolated oscillatorioid cyanobacterium isolated from a mesotrophic reservoir. Phycologia 51:672-83.

Tschoeke, D., Salazar, V. W., Vidal, L., Campeao, M., Swings, J., Thompson, F. & Thompson, C. 2020. Unlocking the genomic taxonomy of the Prochlorococcus collective. Microb. Ecol. 80:546-58.

Tuji, A., Yamaguchi, H., Kataoka, T., Sato, M., Sano, T. & Niiyama, Y. 2021. Annamia dubia sp. nov. with a description of a new family, Geminocystaceae fam. nov. (Cyanobacteria). Fottea 21:100-9.

Uher, B. 2010. Cyanobacterium Petalonema alatum Berk. ex Kirchn. - species variability and diversity. Fottea 10:83-92.

Utami, Y. D., Kuwahara, H., Murakami, T., Morikawa, T., Sugaya, K., Kihara, K., Yuki, M. et al. 2018. Phylogenetic diversity and single-cell genome analysis of "Melainabacteria", a non-photosynthetic cyanobacterial group, in the termite gut. Microbes Environ. 33:50-7.

Vaz, M. G., Genuário, D. B., Andreote, A. P., Malone, C. F., Sant'Anna, C. L., Barbiero, L. & Fiore, M. F. 2015. Pantanalinema gen. nov. and Alkalinema gen. nov.: Novel pseudanabaenacean genera (Cyanobacteria) isolated from saline-alkaline lakes. Int. J. Syst. Evol. Microbiol. 65:298-308.

Vázquez-Martínez, J., Gutierrez-Villagomez, J. M., Fonseca-Garcia, C., Ramírez-Chávez, E., Mondragón-Sánchez, M. A. L., Partida-Martínez, L., Johansen, J. R. & Molina-Torres, J. 2018. Nodosilinea chupicuarensis sp nov. (Leptolyngbyaceae, Synechococcales) a subaerial cyanobacterium isolated from a stone monument in central Mexico. Phytotaxa 334:167-82.

Villanueva, C. D., Hašler, P., Dvořák, P., Poulíčková, A. & Casamatta, D. A. 2018. Brasilonema lichenoides sp. nov. and Chroococcidiopsis lichenoides sp. nov. (Cyanobacteria): two novel cyanobacterial constituents isolated from a tripartite lichen of headstones. J. Phycol. 54:224-33.

Walter, J. M., Coutinho, F. H., Dutilh, B. E., Swings, J., Thompson, F. L. & Thompson, C. C. 2017. Ecogenomics and taxonomy of cyanobacteria phylum. Front. Microbiol. 8:2132.

Wang, Y., Cai, F., Jia, N. & Li, R. 2019. Description of a novel coccoid cyanobacterial genus and species Sinocapsa zengkensis gen. nov. sp. nov. (Sinocapsaceae, incertae sedis), with taxonomic notes on genera in Chroococcidiopsidales. Phytotaxa 409:146-60.

Whatley, J. M. 1977. The fine structure of Prochloron. New Phytol. 79:309-13.

Yu, G., Zhu, M., Chen, Y., Pan, Q., Chai, W. & Li, R. 2015. Polyphasic characterization of four species of Pseudanabaena (Oscillatoriales, Cyanobacteria) from China and insights into polyphyletic divergence within the Pseudanabaena genus. Phytotaxa 192:1-12.

Zammit, G. 2018. Systematics and biogeography of sciophilous cyanobacteria; an ecological and molecular description of Albertania skiophila (Leptolyngbyaceae) gen. & sp. nov. Phycologia 57:481-91.

Zammit, G., Billi, D. & Albertano, P. 2012. The subaerophytic cyanobacterium Oculatella subterranea (Oscillatoriales, Cyanophyceae) gen. et sp. nov.: A cytomorphological and molecular description. Eur. J. Phycol. 47:341-54.

Zapomělová, E., Skácelová, O., Pumann, P., Kopp, R. & Janeček, E. 2012. Biogeographically interesting planktonic Nostocales (Cyanobacteria) in The Czech Republic and their polyphasic evaluation resulting in taxonomic revisions of Anabaena bergii Ostenfeld 1908 (Chrysosporum gen. nov.) and A. tenericaulis Nygaard 1949 (Dolichospermum tenericaule comb. nova). Hydrobiologia 698:353-65.

Zhang, Q. I., Zheng, L., Li, T., Li, R. & Song, L. 2018. Aliterella shaanxiensis (Aliterellaceae), a new coccoid cyanobacterial species from China. Phytotaxa 374:211-20.

Zhou, W. G., Ding, D. W., Yang, Q. S., Ahmad, M., Zhang, Y. Z., Lin, X. C., Zhang, Y. Y., Ling, J. & Dong, J. D. 2018. Marileptolyngbya sina gen. nov., sp. nov. and Salileptolyngbya diazotrophicum gen. nov., sp. nov. (Synechococcales, Cyanobacteria), species of cyanobacteria isolated from a marine ecosystem. Phytotaxa 383:211-20.

Zhu, M. L., Yu, G. L., Li, X. C., Tan, W. H. & Li, R. H. 2012. Taxonomic and phylogenetic evaluation of Limnothrix strains (Oscillatoriales, Cyanobacteria) by adding Limnothrix planktonica strains isolated from central China. Hydrobiologia 698:367-74.

Zimba, P. V., Huang, I. S., Foley, J. E. & Linton, E. W. 2017. Identification of a new-to-science cyanobacterium, Toxifilum mysidocida gen. nov. & sp. nov. (Cyanobacteria, Cyanophyceae). J. Phycol. 53:188-97.

Zimba, P. V., Shalygin, S., Huang, I. S., Momčilović, M. & Abdulla, H. 2021. A new boring toxin producer - Perforafilum tunnelli gen. & sp. nov. (Oscillatoriales, Cyanobacteria) isolated from Laguna Madre, Texas, USA. Phycologia 60:10-24.

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