Biodistribution analyses of nanocarriers are often performed with optical imaging. Though dye tags can interact with transporters, e.g., organic anion transporting polypeptides (OATPs), their influence on biodistribution was hardly studied. Therefore, this study compared tumor cell uptake and biodistribution (in A431 tumor-bearing mice) of four near-infrared fluorescent dyes (AF750, IRDye750, Cy7, DY-750) and dye-labeled poly(N-(2-hydroxypropyl)methacrylamide)-based nanocarriers (dye-pHPMAs). Tumor cell uptake of hydrophobic dyes (Cy7, DY-750) was higher than that of hydrophilic dyes (AF750, IRDye750), and was actively mediated but not related to OATPs. Free dyes' elimination depended on their hydrophobicity, and tumor uptake correlated with blood circulation times. Dye-pHPMAs circulated longer and accumulated stronger in tumors than free dyes. Dye labeling significantly influenced nanocarriers' tumor accumulation and biodistribution. Therefore, low-interference dyes and further exploration of dye tags are required to achieve the most unbiased results possible. In our assessment, AF750 and IRDye750 best qualified for labeling hydrophilic nanocarriers.

Institute for Experimental Molecular Imaging RWTH Aachen University Forckenbeckstrasse 55 52074 Aachen Germany

Institute for Experimental Molecular Imaging RWTH Aachen University Forckenbeckstrasse 55 52074 Aachen Germany; Gremse IT GmbH Dennewartstrasse 25 52068 Aachen Germany

Institute for Experimental Molecular Imaging RWTH Aachen University Forckenbeckstrasse 55 52074 Aachen Germany; Helmholtz Institute for Biomedical Engineering RWTH Aachen University Aachen Germany; Fraunhofer MEVIS Institute for Medical Image Computing Aachen Germany

Institute of Macromolecular Chemistry Czech Academy of Sciences Heyrovského nám 2 162 06 Prague Czech Republic

Citace poskytuje Crossref.org

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