Tuning polymer-blood and polymer-cytoplasm membrane interactions by manipulating the architecture of poly(2-oxazoline) triblock copolymers
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
37742364
DOI
10.1016/j.colsurfb.2023.113564
PII: S0927-7765(23)00442-3
Knihovny.cz E-resources
- Keywords
- Amphiphilic triblock polyoxazoline, Biodistribution in vivo, Blood proteins, Drug delivery, HEK 293 cells, Isothermal titration calorimetry,
- MeSH
- Cell Membrane MeSH
- Cytoplasm MeSH
- HEK293 Cells MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Humans MeSH
- Ligands MeSH
- Micelles * MeSH
- Mice MeSH
- Polymers * chemistry MeSH
- Tissue Distribution MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Ligands MeSH
- Micelles * MeSH
- poly(2-oxazoline) MeSH Browser
- Polymers * MeSH
Bioactive moieties designed to bind to cell membrane receptors benefit from coupling with polymeric carriers that have enhanced affinity to the cell membrane. When bound to the cell surface, such carriers create a "2D solution" of a ligand with a significantly increased concentration near a membrane-bound receptor compared to a freely water-soluble ligand. Bifunctional polymeric carriers based on amphiphilic triblock copolymers were synthesized from 2-pent-4-ynyl oxazoline, 2-nonyl oxazoline and 2-ethyl oxazoline. Their self-assembly and interactions with plasma proteins and HEK 293 cells were studied in detail. The affinity of these triblock copolymers to HEK 293 cell membranes and organ tissues was tunable by the overall hydrophobicity of the polymer molecule, which is determined by the length of the hydrophobic and hydrophilic blocks. The circulation time and biodistribution of three representative triblock copolymers were monitored after intravenous administration to C57BL/6 albino mice. A prolonged circulation time was observed for polymers with longer hydrophobic blocks, despite their molecular weight being below the renal threshold.
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