The genus Vigna (Leguminosae) comprises about 150 species grouped into five subgenera. The present study aimed to improve the understanding of karyotype diversity and evolution in Vigna, using new and previously published data through different cytogenetic and DNA content approaches. In the Vigna subgenera, we observed a random distribution of rDNA patterns. The 35S rDNA varied in position, from terminal to proximal, and in number, ranging from one (V. aconitifolia, V. subg. Ceratotropis) to seven pairs (V. unguiculata subsp. unguiculata, V. subg. Vigna). On the other hand, the number of 5S rDNA was conserved (one or two pairs), except for V. radiata (V. subg. Ceratotropis), which had three pairs. Genome size was relatively conserved within the genus, ranging from 1C = 0.43 to 0.70 pg in V. oblongifolia and V. unguiculata subsp. unguiculata, respectively, both belonging to V. subg. Vigna. However, we observed a positive correlation between DNA content and the number of 35S rDNA sites. In addition, data from chromosome-specific BAC-FISH suggest that the ancestral 35S rDNA locus is conserved on chromosome 6 within Vigna. Considering the rapid diversification in the number and position of rDNA sites, such conservation is surprising and suggests that additional sites may have spread out from this ancestral locus.

Campus Amilcar Ferreira Sobral Universidade Federal Do Piauí Floriano PI Brazil

Departamento de Botânica Centro de Biociências Universidade Federal de Pernambuco Recife PE Brazil

Departamento de Genética Centro de Biociências Universidade Federal de Pernambuco Recife PE Brazil

Department of Biology McMaster University 1280 Main Street West Hamilton ON L8S4K1 Canada

Department of Cell Biology Faculty of Science Charles University Viničná 7 Prague 12843 Czech Republic

Instituto Agronômico de Pernambuco Recife PE Brazil

Instituto Tecnológico Vale Belém PA Brazil

Laboratório de Imunopatologia Keizo Asami Universidade Federal de Pernambuco Recife PE Brazil

Universidade Do Estado de Minas Gerais Unidade Divinópolis Divinópolis MG Brazil

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