The contamination of food and animal feeds with mycotoxions, particularly aflatoxin B1 (AFB1), poses significant risks to human health and causes economic losses. This study investigated bacteria from various fermented milk products to assess their ability to detoxify AFB1. A variety of household fermented kefir milk, kefir-like beverages, and kefir grains were collected from rural areas and subjected to microbiological analysis. Gram-positive bacterial isolates were further identified based on the 16S rRNA gene homology analysis. Seven bacterial isolates that were initially identified as lactic acid bacteria were selected for their potential to detoxify AFB1. Effects of environmental factors, including temperature, time, pH, and cell concentration, as well as bacterial components such as inoculum, fermentation supernatant, and cells, were evaluated on AFB1 detoxification. The most frequent isolates belonged to the new genus Lentilactobacillus and Lactiplantibacillus, of which three strains were identified as L. kefiri, L. diolivorans, and L. plantarum. The selected L. plantarum isolate demonstrated optimal AFB1 detoxification at pH 4, a 4-h exposure time, and a cell concentration of 1.0 × 1016 CFU/mL. Significant differences were observed in toxin removal between fermentation supernatant and cells, while temperature showed no significant effect on toxin detoxification. This study demonstrated the high ability of L. plantarum for AFB1 detoxification, suggesting potential applications for food and feed safety enhancement. Further research is warranted to optimize its effectiveness and explore broader applications.

Department of Medical Parasitology and Mycology Faculty of Para Medicine Golestan University of Medical Sciences Gorgan Iran

Department of Microbiology Ayatollah Amoli Branch Islamic Azad University Amol Iran

Department of Microbiology Faculty of Medicine Golestan University of Medical Sciences Gorgan Iran

Infectious Diseases Research Center Golestan University of Medical Sciences Gorgan Iran

Laboratory Sciences Research Center Golestan University of Medical Sciences Gorgan Iran

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