RNA secondary (2D) structure visualisation is an essential tool for understanding RNA function. R2DT is a software package designed to visualise RNA 2D structures in consistent, recognisable, and reproducible layouts. The latest release, R2DT 2.0, introduces multiple significant features, including the ability to display position-specific information, such as single nucleotide polymorphisms (SNPs) or SHAPE reactivities. It also offers a new template-free mode allowing visualisation of RNAs without pre-existing templates, alongside a constrained folding mode and support for animated visualisations. Users can interactively modify R2DT diagrams, either manually or using natural language prompts, to generate new templates or create publication-quality images. Additionally, R2DT features faster performance, an expanded template library, and a growing collection of compatible tools and utilities. Already integrated into multiple biological databases, R2DT has evolved into a comprehensive platform for RNA 2D visualisation, accessible at https://r2dt.bio.

Department of Biomolecular Engineering Baskin School of Engineering University of California Santa Cruz CA 95064 USA

Department of Cell Biology and Molecular Genetics University of Maryland College Park MD 20742 USA

Department of Mathematics and Statistics Bowling Green State University Bowling Green OH 43403 USA

Department of Software Engineering Charles University Prague 118 00 Czech Republic

European Molecular Biology Laboratory Wellcome Genome Campus European Bioinformatics Institute Hinxton Cambridge CB10 1SD UK

Faculty of Life Sciences University of Strasbourg Strasbourg 67000 France

NASA Center for Integration of the Origin of Life Georgia Institute of Technology Atlanta GA 30332 0400 USA; School of Biological Sciences Georgia Institute of Technology Atlanta GA 30332 0400 USA

National Center for Biotechnology Information U S National Library of Medicine National Institutes of Health Bethesda MD 20894 USA

Riboscope Ltd 23 King Street Cambridge CB1 1AH UK

Unaffiliated

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Nucleic Acids Res. 2025 Feb 8;53(4):gkaf032. doi: 10.1093/nar/gkaf032. PubMed

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