Here, we present surface analysis and biocompatibility evaluation of novel composite material based on graphene oxide traded as BioHastalex. The pristine material's surface morphology and surface chemistry were examined by various analytical methods. The BioHastalex with a thin silver layer was subsequently heat treated and characterized, the impact on the material surface wettability and morphology was evaluated. Significant surface roughness and morphology changes were detected at the nanometer scale after heat treatment of Ag-sputtered BioHastalex. The deposition of a thin silver nanolayer had an outstanding effect on BioHastalex's antibacterial properties while still maintaining cell viability (MRC-5, HaCaT). The heat treatment of BioHastalex-Ag led to the formation of regular nanocluster arrays while affecting the Ag concentration on the very surface. The decrease in silver concentration was connected with the length of heat treatment; cells growing on such samples exhibited good viability, and the antibacterial properties were weaker than simply sputtered BioHastalex.

Department of Biochemistry and Microbiology University of Chemistry and Technology Prague 166 28 Prague Czech Republic

Department of Inorganic Chemistry University of Chemistry and Technology Prague 166 28 Prague Czech Republic

Department of Solid State Engineering University of Chemistry and Technology Prague 166 28 Prague Czech Republic

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