OBJECTIVES: This study aimed to investigate the acrylamide exposure of different children and adult population groups (10-14 years, 15-17 years, 18-24 years, 25-44 years, 45-64 years, and 65-74 years) resulting from the consumption of potato chips and wheat-based bread from Montenegrin market and to evaluate it in terms of health risk. METHODS: The acrylamide content was monitored in 51 samples of bread and 20 samples of chips. The carcinogenic health risk in different population groups was assessed through the incremental lifetime cancer risk (CR) and total cancer risk (TCR). RESULTS: The average acrylamide content in potato chips and bread was calculated to be 238 μg/kg and 30 μg/kg, respectively. Acrylamide content in a tested sample met the criteria prescribed by Commission Regulation (EU) 2017/2158 in 98% of the tested samples of chips and 85% of bread samples. The carcinogenic health risk of acrylamide exposure for the investigated population groups is of concern. The values of CR for all the investigated groups were in the range of 10-6 < CR < 10-4 and the values of TCR were 10-5 order of magnitude, indicating a potential cancer risk. CONCLUSION: The youngest population (10-14 years) was exposed to the highest cancer risk through the consumption of both, chips and bread. For the population of 10-14 years, 15-17 years, 25-44 years, 45-64 years, and 65-74 years, a higher risk of cancer was found due to the consumption of bread compared to the consumption of chips. Only the population aged 18-24 years was faced with a higher risk of cancer due to the consumption of chips compared to the consumption of bread.

Biotechnical Faculty University of Montenegro Podgorica Montenegro

Faculty for Food Technology Food Safety and Ecology University Donja Gorica Podgorica Montenegro

Faculty of Science and Mathematics University of Montenegro Podgorica Montenegro

Institute of Public Health of Montenegro Podgorica Montenegro

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Shipp A, Lawrence G, Gentry R, McDonald T, Bartow H, Bounds J, et al. Acrylamide: review of toxicity data and dose-response analyses for cancer and noncancer effects. Crit Rev Toxicol. 2006 Jul-Aug;36(6-7):481-608.

Joint FAO/WHO Expert Committee on Food Additives. Evaluation of certain food contaminants: sixty-fourth report of the Joint FAO/WHO Expert Committee on Food Additives. World Health Organ Tech Rep Ser. 2006:930;1-100.

Ahn JS, Castle L, Clarke DB, Lloyd AS, Philo MR, Speck DR. Verification of the findings of acrylamide in heated foods. Food Addit Contam. 2002 Dec;19(12):1116-24.

EFSA Panel on Contaminants in the Food Chain (CONTAM). Scientific opinion on acrylamide in food. EFSA J. 2015;13(6):4104. doi: 10.2903/j.efsa.2015.4104. DOI

Bušová M, Bencko V, Kromerová K, Nadjo I, Babjaková J. Occurrence of acrylamide in selected food products. Cent Eur J Public Health. 2020;28(4):320-4.

Romani S, Bacchiocca M, Rocculi P, Dalla Rosa M. Effect of frying time on acrylamide content and quality aspects of French fries. Eur Food Res Technol. 2008;226(3):555-60.

Hagmar L, Törnqvist M, Nordander C, Rosén I, Bruze M, Kautiainen A, et al. Health effects of occupational exposure to acrylamide using hemoglobin adducts as biomarkers of internal dose. Scand J Work Environ Health. 2001;27(4):219-26.

World Health Organization. Health implications of acrylamide in food: report of a joint FAO/WHO consultation; WHO Headquarters, Geneva, Switzerland; 25-27 June 2002. Geneva: WHO; 2002.

Zamani E, Shokrzadeh M, Fallah M, Shaki F. A review of acrylamide toxicity and its mechanism. Pharm Biomed Res. 2017;3(1):1-7.

IARC. IARC monographs on the evaluation of carcinogenic risks to humans. Acrylamide: summary of data reported and evaluation. Lyon: IARC; 1994.

Commission Regulation (EU) 2017/2158 of 20 November 2017 establishing mitigation measures and benchmark levels for the reduction of the presence of acrylamide in food. Off J Eur Union. 2017 Nov 21;60(L 304):24-44.

Directive (EU) 2020/2184 of the European Parliament and of the Council of 16 December 2020 on the quality of water intended for human consumption (recast). Off J Eur Union. 2020 Dec 23;63(L 435):1-62.

Stefanović S, Đorđević V, Jelušić V. Rapid and reliable QuEChERS-based LC-MS/MS method for determination of acrylamide in potato chips and roasted coffee. IOP Conf Ser Earth Environ Sci. 2017:85;012010. doi: 10.1088/1755-1315/85/1/012010. DOI

BS EN 15662:2018-TC. Foods of plant origin - Multimethod for the determination of pesticide residues using GC- and LC-based analysis following acetonitrile extraction/partitioning and clean-up by dispersive SPE - Modular QuEChERS-method. London: British Standards Institution; 2020.

United States Environmental Protection Agency. Risk assessment guidance for superfund. Volume 1: Human health evaluation manual, part A. Interim final [Internet]. USEPA: Washington, D.C.; 1989 [cited 2024 Sep 18]. Available from: https://www.epa.gov/system/files/documents/2024-10/rags_a_508.pdf.

Rufián-Henares JA, Morales FJ. Determination of acrylamide in potato chips by a reversed-phase LC-MS method based on a stable isotope dilution assay. Food Chem. 2006;97(3):555-62.

Arisseto AP, Toledo MC de F, Govaert Y, van Loco J, Fraselle S, Degroodt JM, et al. Contribution of selected foods to acrylamide intake by a population of Brazilian adolescents. LWT Food Sci Technol. 2009;42(1):207-11.

Shamla L, Nisha P. Acrylamide in deep-fried snacks of India. Food Addit Contam Part B Surveill. 2014;7(3):220-5.

Becalski A, Stadler R, Hayward S, Kotello S, Krakalovich T, Lau BPY, et al. Antioxidant capacity of potato chips and snapshot trends in acrylamide content in potato chips and cereals on the canadian market. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2010 Sep;27(9):1193-8.

European Food Safety Authority. Results on acrylamide levels in food from monitoring years 2007-2009 and exposure assessment. EFSA J. 2011;9(4):2133. doi: 10.2903/j.efsa.2011.2133. DOI

Murkovic M. Acrylamide in Austrian foods. J Biochem Biophys Methods. 2004;61(1-2):161-7.

Pogurschi EN, Zugravu CA, Ranga IN, Trifunschi S, Munteanu MF, Popa DC, et al. Determination of acrylamide in selected foods from the Romanian market. Foods. 2021;10(9):2110. doi: 10.3390/foods10092110. DOI

Mojska H, Gielecińska I, Szponar L, Ołtarzewski M. Estimation of the dietary acrylamide exposure of the Polish population. Food Chem Toxicol. 2010;48(8-9):2090-6.

Hilbig A, Freidank N, Kersting M, Wilhelm M, Wittsiepe J. Estimation of the dietary intake of acrylamide by German infants, children and adolescents as calculated from dietary records and available data on acrylamide levels in food groups. Int J Hyg Environ Health. 2004;207(5):463-71.

Govaert Y, Arisseto A, Van Loco J, Scheers E, Fraselle S, Weverbergh E, et al. Optimisation of a liquid chromatography-tandem mass spectrometric method for the determination of acrylamide in foods. Anal Chim Acta. 2006;556(2):275-80.

Mencin M, Abramovič H, Vidrih R, Schreiner M. Acrylamide levels in food products on the Slovenian market. Food Control. 2020;114:107267. doi: 10.1016/j.foodcont.2020.107267. DOI

Abt E, Robin LP, McGrath S, Srinivasan J, DiNovi M, Adachi Y, et al. Acrylamide levels and dietary exposure from foods in the United States, an update based on 2011-2015 data. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2019 Oct;36(10):1475-90.

Cieslik I, Cieslik E, Topolska K, Surma M. Dietary acrylamide exposure from traditional food products in lesser Poland and associated risk assessment. Ann Agric Environ Med. 2020;27(2):225-30.