The barbels of the subfamilies ´Poropuntinae´ and Smiliogastrinae within the family Cyprinidae play a significant role as a food source for fish in artisanal fisheries and are highly valued as ornamental fish in Thailand. In this study, we employed both conventional and molecular cytogenetics to analyze the karyotype of 15 fish species from two cyprinid lineages. All analyzed species had a diploid chromosome number of 2n = 50. Despite sharing the same 2n, our analyses revealed species-specific distribution patterns of the mapped microsatellite motifs [(CA)₁₅, (TA)₁₅, (CAC)₁₀, and (CGG)₁₀]. They were predominantly found at telomeric sites of all-to-few chromosomes. Additionally, some species exhibited a widespread distribution of the mapped microsatellites across the chromosomes while others showed no signal. These variations reflect the evolutionary divergence and chromosomal diversity within the cyprinids. Thus, our findings support the 2n stability in cyprinoid lineages while emphasizing the intrachromosomal evolutionary diversity accompanied by species-specific microsatellite distribution.

Deceased

Departamento de Genética e Evolução Universidade Federal de São Carlos São Carlos São Paulo Brazil

Department of Biology Faculty of Science Khon Kaen University Muang Khon Kaen 40002 Thailand

Department of Conservation Biology Mahidol University Kanchanaburi Campus Sai Yok Kanchanaburi 71150 Thailand

Department of Fisheries Faculty of Agricultural Technology Sakon Nakhon Rajabhat University Sakon Nakhon 47000 Thailand

Department of Program in Animal Science Faculty of Agricultural Technology and Agro industry Rajamangala University of Technology Suvarnabhumi Phra Nakhon Si Ayutthaya Ayutthaya 13000 Thailand

Department of Science Faculty of Science and Technology Prince of Songkla University Pattani Campus Mueng Pattani 94000 Thailand

Division of Biology Department of Science Faculty of Science and Technology Rajamangala University of Technology Krungthep Bangkok 10120 Thailand

Faculty of Interdisciplinary Studies Nong Khai Campus Khon Kaen University Muang Nong Khai 43000 Thailand

Institute of Animal Physiology and Genetics Laboratory of Fish Genetics Czech Academy of Sciences Rumburska´ Liběchov Czech Republic

Institute of Human Genetics Jena University Hospital Friedrich Schiller University 07747 Jena Germany

School of Agriculture and Natural Resources University of Phayao Tumbol Maeka Muang District Phayao Province 56000 Thailand

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