The 11th International Symposium on Phlebotomine Sand flies (ISOPS XI) took place in Portorož, Slovenia, in September 2024 and brought together experts from around the world to discuss recent advances in the biology, ecology and control of phlebotomine sand flies and the pathogens they transmit. This report summarises the key findings of the symposium and is organised thematically by session. Key topics included the development of refined experimental models of Leishmania transmission, new insights into the interactions between vector, parasite and microbiota, and the detection of Leishmania donovani in new geographic regions. Advances in molecular diagnostics and surveillance technologies were emphasised, as were emerging concerns about insecticide resistance. The potential of paratransgenesis and symbiont-based vector control approaches was also emphasised. In a separate session, the CLIMOS project was presented, which integrates climate monitoring, ecological modelling and public health tools to develop an early warning system (EWS) for sand fly-borne diseases. Overall, the contributions to the symposium reflect the dynamic development of sand fly research in response to global environmental change and emphasise the importance of international collaboration in combating emerging vector-borne diseases.

TITLE: Progrès récents à propos de la recherche sur les phlébotomes : une revue basée sur les études présentées à ISOPS XI. ABSTRACT: Le 11e Symposium international sur les phlébotomes (ISOPS XI) s’est tenu à Portorož, en Slovénie, en septembre 2024. Il a réuni des experts du monde entier pour discuter des avancées récentes en biologie, en écologie et en lutte contre les phlébotomes et les agents pathogènes qu’ils transmettent. Cette revue résume les principaux résultats présentés lors du symposium, organisés par session thématique. Les principaux sujets abordés comprenaient le développement de modèles expérimentaux affinés de transmission de Leishmania, de nouvelles connaissances sur les interactions vecteur-parasite-microbiote et la détection de Leishmania donovani dans de nouvelles régions géographiques. Les avancées en matière de diagnostic moléculaire et de technologies de surveillance ont été mises en avant, ainsi que les préoccupations émergentes concernant la résistance aux insecticides. Le potentiel de la paratransgénèse et des approches de lutte antivectorielle basées sur les symbiotes a également été souligné. Une session dédiée a présenté le projet CLIMOS, qui intègre la surveillance du climat, la modélisation écologique et des outils de santé publique pour développer un système d’alerte précoce (SAP) pour les maladies transmises par les phlébotomes. Collectivement, les contributions du symposium reflètent l’évolution dynamique de la recherche sur les phlébotomes face aux changements environnementaux mondiaux et soulignent l’importance de la collaboration internationale pour faire face aux menaces émergentes de maladies vectorielles.

Department of Infectious Diseases Vector Borne Diseases Unit Istituto Superiore di Sanità 00161 Rome Italy

Department of Natural Sciences University of Maryland Eastern Shore Princess Anne MD 21853 USA

Department of Parasitology Faculty of Medicine Ege University 35100 Bornova Izmir Türkiye

Department of Parasitology Faculty of Science Charles University 128 00 Prague 2 Czech Republic

Department of Parasitology Institute of Biomedical Sciences University of São Paulo 05508 000 São Paulo Brazil

Faculté de Pharmacie Université de Reims Champagne Ardenne UR ESCAPE USC ANSES PETARD 51097 Reims Cedex France

Faculty of Mathematics Natural Sciences and Information Technologies University of Primorska 6000 Koper Slovenia

Global Health and Tropical Medicine 1349 008 Lisboa Portugal

Institute for Medical Research University of Belgrade 11000 Belgrade Serbia

Laboratório de Biologia Molecular de Parasitos e Vetores Instituto Oswaldo Cruz Fiocruz 21040 360 Rio de Janeiro RJ Brazil

Vector Biology and Vector Borne Disease Research Unit Department of Parasitology Faculty of Medicine Chulalongkorn University 10330 Bangkok Thailand

Vector Molecular Biology Section Laboratory of Malaria and Vector Research National Institute of Allergy and Infectious Diseases National Institutes of Health Rockville MD 20852 USA

Zobrazit více v PubMed

Asfaram S, Fakhar M, Mohebali M, Ziaei Hezarjaribi H, Mardani A, Ghezelbash B, Akhoundi B, Zarei Z, Moazeni M. 2022. A convenient and sensitive kDNA-PCR for screening of PubMed

Ayhan N, Baronti C, Thrion L, Bongiorno G, Maia C, CLIMOS WP2 Phlebovirus Working Group. 2025. External quality assessment for molecular detection of sand fly-borne phleboviruses circulating in the Mediterranean Basin. Parasites & Vectors, 18, 173. PubMed PMC

Balaska S, Fotakis EA, Chaskopoulou A, Vontas J. 2021. Chemical control and insecticide resistance status of sand fly vectors worldwide. PLOS Neglected Tropical Diseases, 15, e0009586. PubMed PMC

Bamorovat M, Sharifi I, Khosravi A, Aflatoonian MR, Agha Kuchak Afshari S, Salarkia E, Sharifi F, Aflatoonian B, Gharachorloo F, Khamesipour A, Mohebali M, Zamani O, Shirzadi MR, Gouya MM. 2024. Global dilemma and needs assessment toward achieving sustainable development goals in controlling leishmaniasis. Journal of Epidemiology and Global Health, 14, 22–34. PubMed PMC

Belkaid Y, Kamhawi S, Modi G, Valenzuela J, Noben-Trauth N, Rowton E, Ribeiro J, Sacks DL. 1998. Development of a natural model of cutaneous leishmaniasis: powerful effects of vector saliva and saliva preexposure on the long-term outcome of PubMed PMC

Bezerra-Santos MA, Zeni V, Pistillo OM, Bedini S, D’Isita I, Benelli G, Germinara GS, Volf P, Otranto D.. 2024. PubMed PMC

Benallal KE, Mefissel M, Dib Y, Depaquit J, Kavan D, Harrat Z, Dvořák V, Volf P, Halada P. 2024. Phlebotomine sand fly survey, blood meal source identification, and description of PubMed PMC

Bourdeau P, Rowton E, Petersen C. 2020. Impact of different PubMed PMC

Brake S, Gomez-Maldonado D, Hummel M, Zohdy S, Peresin MS. 2022. Understanding the current state-of-the-art of long-lasting insecticide nets and potential for sustainable alternatives. Current Research in Parasitology and Vector-Borne Diseases, 2, 100101. PubMed PMC

Catta-Preta CMC, Sacks DL. 2025. Genetic exchange in PubMed DOI

Cecilio P, Iniguez E, Huffcutt P, Ribeiro SP, Kamhawi S, Valenzuela JG, Serafim TD. 2025. The impact of blood on vector-borne diseases with emphasis on mosquitoes and sand flies. Trends in Parasitology, 41, 196–209. PubMed PMC

Cecilio P, Rogerio LA, Serafim TD, Tang K, Willen L, Iniguez E, Meneses C, Chaves LF, Zhang Y, Dos Santos Felix L, Huang W, Garcia Guizzo M, Castañeda-Casado P, Jacobs-Lorena M, Valenzuela JG, Rodrigues J, Oliveira F. 2025. PubMed PMC

de Sousa Félix de Lima M, Albuquerque e Silva R, de Almeida Rocha D, de Oliveira Mosqueira G, Gurgel-Gonçalves R, Takashi Obara M. 2024. Insecticide-impregnated dog collars for the control of visceral leishmaniasis: evaluation of the susceptibility of field PubMed PMC

Di Giorgio C, Delmas F, Ollivier E, Elias R, Balansard G, Timon-David P. 2004. PubMed

Dutra-Rêgo F, Binder C, Capucci DC, Vaz TP, Andrade Filho JD, Fontes G, Gontijo CMF. 2024. Diversity, PubMed PMC

Fan D, Zhao T, Jiang X, García-García A, Schmidt T, Samaniego L, Attinger S, Wu H, Jiang Y, Shi J, Fan L, Tang B-H, Wagner W, Dorigo W, Gruber A, Mattia F, Balenzano A, Brocca L, Jagdhuber T, Wigneron J-P, Montzka C, Peng J. 2025. A sentinel-1 SAR-based global 1-km resolution soil moisture data product: algorithm and preliminary assessment. Remote Sensing of Environment, 318, 114579.

Giraud E, Martin O, Yakob L, Rogers M. 2019. Quantifying PubMed PMC

Hassan MI, Al-Sawaf BM, Fouda MA, Al-Hosry S, Hammad KM. 2014. A recent evaluation of the sand fly,

Huang W, Rodrigues J, Bilgo E, Tormo JR, Challenger JD, De Cozar-Gallardo C, Pérez-Victoria I, Reyes F, Castañeda-Casado P, Gnambani EJ, Hien DFS, Konkobo M, Urones B, Coppens I, Mendoza-Losana A, Ballell L, Diabate A, Churcher TS, Jacobs-Lorena M. 2023. PubMed

Jancarova M, Polanska N, Thiesson A, Arnaud F, Stejskalova M, Rehbergerova M, Kohl A, Viginier B, Volf P, Ratinier M. 2025. Susceptibility of diverse sand fly species to Toscana virus. PloS Neglected Tropical Diseases, 19, e0013031. PubMed PMC

Kapwata T, Wright CY, du Preez DJ, Kunene Z, Mathee A, Ikeda T, Landman W, Maharaj R, Sweijd N, Minakawa N, Blesic S. 2021. Exploring rural hospital admissions for diarrhoeal disease, malaria, pneumonia, and asthma in relation to temperature, rainfall and air pollution using wavelet transform analysis. Science of the Total Environment, 791, 148307. PubMed

Kato H. 2025. Dr. Jekyll and Mr. Hyde in sand fly saliva. Parasitology International, 105, 102998. PubMed

Khogali A, Elnaiem DA, Díaz-Regañón R, Jibreel T, Nour BYM, Abdelrahman SH, Molina R, Jiménez M. 2024. Infection of PubMed PMC

Kumar R, Engwerda C. 2014. Vaccines to prevent leishmaniasis. Clinical and Translational Immunology, 3, e13. PubMed PMC

Kumari Y, Gunathilaka N, Amarasinghe D. 2025. A comprehensive review of biological and genetic control approaches for leishmaniasis vector sand flies; emphasis towards promoting tools for integrated vector management. PLOS Neglected Tropical Diseases, 19, e0012795. PubMed PMC

Lestinova T, Rohousova I, Sima M, de Oliveira CI, Volf P. 2017. Insights into the sand fly saliva: blood-feeding and immune interactions between sand flies, hosts, and PubMed PMC

Louradour I, Monteiro CC, Inbar E, Ghosh K, Merkhofer R, Lawyer P, Sacks D. 2017. The midgut microbiota plays an essential role in sand fly vector competence for PubMed PMC

Marialva EF, Martins-Campos KM, de Almeida VR, Ríos-Velasquez CM, Tempone AJ, Pessoa FAC, Traub-Cseko YM. 2025. Investigation of bacterial microbiota variability in two allopatric populations of PubMed PMC

Molina R, Jimenez MI, Cruz I, Iriso A, Martin-Martin I, Sevillano O, Melero S, Bernal J. 2012. The hare ( PubMed

Moraes CS, Seabra SH, Castro DP, Brazil RP, de Souza W, Garcia ES, Azambuja P. 2008. PubMed

Moraes CS, Seabra SH, Albuquerque-Cunha JM, Castro DP, Genta FA, de Souza W, Azambuja P. 2009. Prodigiosin is not a determinant factor in lysis of PubMed

N’Guessan R, Odjo A, Ngufor C, Malone D, Rowland M. 2016. A chlorfenapyr mixture net Interceptor G2 shows high efficacy and wash durability against resistant mosquitoes in West Africa. PloS One, 11, e0165925. PubMed PMC

Nzelu CO, Bahrami S, Lawyer PG, Peters NC. 2025. Detection of PubMed PMC

Otranto D, Dantas-Torres F. 2013. The prevention of canine leishmaniasis and its impact on public health. Trends in Parasitology, 29, 339–345. PubMed

Pan American Health Organization. 2024. Plan of action to strengthen the surveillance and control of leishmaniasis in the Americas 2023–2030. Washington, DC: PAHO.

Prudhomme J, Delabarre A, Alten B, Berberoglu U, Berriatua E, Bongiorno G, Cristovao JM, Davidovich-Cohen M, Di Muccio T, Erisoz Kasap O, Fiorentino E, Kirstein OD, Kniha E, Maia C, Mungan M, Muñoz-Hernández C, Nalçaci M, Oguz Kaskan G, Ozbel Y, Ozensoy Toz S, Parreira R, Platzgummer K, Polat C, Risueño J, Studentsky L, Varol G, Walochnik J, Yetişmiş K, Robert-Gangneux F. 2024. Performance evaluation of nine reference centers and comparison of DNA extraction protocols for effective surveillance of PubMed PMC

Ruiz-Fons F, Ferroglio E, Gortázar C. 2013. PubMed

Sadlova J, Hoskova A, Vojtkova B, Becvar T, Volf P. 2024. The development of PubMed PMC

Sant’Anna MR, Diaz-Albiter H, Aguiar-Martins K, Al Salem WS, Cavalcante RR, Dillon VM, Genta FA, Mazzoni CJ, Bates PA, Dillon RJ. 2014. Colonisation resistance in the sand fly gut: PubMed PMC

Shaw JJ. 2025. A review of PubMed PMC

Sovi A, Adoha CJ, Yovogan B, Cross CL, Dee DP, Konkon AK, Sidick A, Accrombessi M, Ahouandjinou MJ, Ossè R, Dangbénon E, Towakinou L, Agbangla C, Padonou GG, Churcher TS, Ngufor C, Cook J, Protopopoff N, Akogbéto MC, Messenger LA. 2024. The effect of next-generation, dual-active-ingredient, long-lasting insecticidal net deployment on insecticide resistance in malaria vectors in Benin: results of a 3-year, three-arm, cluster-randomised, controlled trial. Lancet Planetary Health, 8, e894–e905. PubMed

Studentsky L, Orshan L, Akad F, Ben Avi I, Diaz D, Elbaz S, Sagi O, Zagron G, Valinsky L, Davidovich-Cohen M, Baneth G. 2023. PubMed PMC

Tempone AJ, Spelta GI, Ramos de Almeida V, Giglioti DM, Costa EM, Mathias I, Vargas H, Lemos-Silva T, Ribeiro ACPDS, Marialva EF, Rios-Velasquez C, Batista M, Santos MDMD, Pessoa FAC, Traub-Csekö YM. 2025. Comparative gut proteome of PubMed PMC

Titus RG, Ribeiro JM. 1988. Salivary gland lysates from the sand fly PubMed

Torrence C, Compo GP. 1998. A practical guide to wavelet analysis. Bulletin of the American Meteorological Society, 79, 61–78.

Volf P, Pruzinova K, Yanase R, Sunter JD. 2025. Midgut and stomodeal valve attachment of PubMed

Wang D, Hof AR, Matson KD, van Langevelde F. 2025. Understanding and predicting the geographic distributions of phlebotomine sand flies in and around Europe (Version 2). Research Square, preprint, posted 9 July 2025, 10.21203/rs.3.rs-6670769/v2. DOI

Weslati M, Ghrab J, Benabid M, Souissi O, Aoun K, Bouratbine A. 2022. Diversity, abundance and PubMed PMC

WHO. 2010. Control of the leishmaniases: report of a meeting of the WHO Expert Committee on the Control of Leishmaniases, Geneva, 22–26 March 2010. Geneva: World Health Organization.