Preparation of Diplonemid Samples for Microscopy
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
- Klíčová slova
- Cell fixation, Cell permeabilization, Endocytosis assay, Fluorescence in situ hybridization, Fluorescence microscopy, Fluorescence staining, Immunofluorescence assay, Live-cell imaging,
- MeSH
- endocytóza MeSH
- fluorescenční mikroskopie metody MeSH
- fluorescenční protilátková technika metody MeSH
- hybridizace in situ fluorescenční metody MeSH
- lidé MeSH
- mikroskopie * metody MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
This chapter provides a comprehensive set of protocols for preparing diplonemid cells for a range of microscopy applications, including live-cell imaging, fixed-cell fluorescence microscopy, endocytosis assay, immunofluorescence assay (IFA), and fluorescence in situ hybridization (FISH). It presents detailed instructions for slide preparation, cell harvesting, chemical fixation, permeabilization, and fluorescent labelling, with critical steps highlighted throughout. These protocols are designed to be adaptable for different experimental goals, enabling researchers to obtain high-quality imaging data for exploring the cellular biology of diplonemids.
Faculty of Science University of South Bohemia České Budějovice Czech Republic
Institute of Cell Biology School of Biological Sciences University of Edinburgh Edinburgh UK
Institute of Evolutionary Biology Faculty of Biology University of Warsaw Warsaw Poland
Institute of Parasitology Biology Centre Czech Academy of Sciences České Budějovice Czech Republic
Zobrazit více v PubMed
Tashyreva D, Prokopchuk G, Yabuki A et al (2018) Phylogeny and morphology of new diplonemids from Japan. Protist 169:158–179. https://doi.org/10.1016/j.protis.2018.02.001 PubMed DOI
Tashyreva D, Prokopchuk G, Votýpka J et al (2018) Life cycle, ultrastructure, and phylogeny of new diplonemids and their endosymbiotic bacteria. MBio 9. https://doi.org/10.1128/mbio.02447-17
Prokopchuk G, Tashyreva D, Yabuki A et al (2019) Morphological, ultrastructural, motility and evolutionary characterization of two new Hemistasiidae species. Protist 170:259–282. https://doi.org/10.1016/j.protis.2019.04.001 PubMed DOI
Marande W, Lukeš J, Burger G (2005) Unique mitochondrial genome structure in diplonemids, the sister group of kinetoplastids. Eukaryot Cell 4:1137–1146. https://doi.org/10.1128/EC.4.6.1137-1146.2005 PubMed DOI PMC
Valach M, Moreira S, Petitjean C et al (2023) Recent expansion of metabolic versatility in Diplonema papillatum, the model species of a highly speciose group of marine eukaryotes. BMC Biol 21:99. https://doi.org/10.1186/s12915-023-01563-9 PubMed DOI PMC
Tashyreva D, Týč J, Horák A et al (2023) Ultrastructure and 3D reconstruction of a diplonemid protist (Diplonemea) and its novel membranous organelle. MBio 14:e0192123. https://doi.org/10.1128/mbio.01921-23 PubMed DOI
Lukeš J, Wheeler R, Jirsová D et al (2018) Massive mitochondrial DNA content in diplonemid and kinetoplastid protists. IUBMB Life 70:1267–1274. https://doi.org/10.1002/iub.1894 PubMed DOI PMC
Prokopchuk G, Korytář T, Juricová V et al (2022) Trophic flexibility of marine diplonemids - switching from osmotrophy to bacterivory. ISME J 16:1409–1419. https://doi.org/10.1038/s41396-022-01192-0 PubMed DOI PMC
Akiyoshi B, Faktorová D, Lukeš J (2025) Discovery of unique mitotic mechanisms in Paradiplonema papillatum. Open Biol 15:250096. https://doi.org/10.1098/rsob.250096
