Nucleic Acid Denaturation [denaturace nukleových kyselin]

topical
62
Terms

denaturace DNA
denaturace RNA
nukleové kyseliny - denaturace
tání DNA

 

DNA Denaturation
DNA Melting
RNA Denaturation

Persistent link   https://www.medvik.cz/link/D009691
Definition

Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is melted by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.

Annotation
IM general only; coord NIM with DNA or RNA or specific (IM)
DUI
D009691 MeSH Browser
CUI
M0015049
History note
70(67)
Public note
70

Allowable subheadings

DE
drug effects 5
GE
genetics 1
PD
pharmacology
RE
radiation effects 2

E Analytical, Diagnostic and Therapeutic Techniques and Equipment
E05 Investigative Techniques 209
E05.393 Genetic Techniques 579
E05.393.085 Cellular Reprogramming Techniques 5
E05.393.128 Chemogenetics
E05.393.170 Chromatin Immunoprecipitation 32
E05.393.183 Chromosome Mapping 427
E05.393.220 Cloning, Molecular 406
E05.393.240 Cloning, Organism 97
E05.393.260 Contig Mapping 9
E05.393.281 Crosses, Genetic 96
E05.393.285 Cytogenetic Analysis 602
E05.393.290 DNA Fingerprinting 139
E05.393.300 DNA Footprinting 12
E05.393.332 Gene Expression Profiling 809
E05.393.335 Gene Targeting 28
E05.393.350 Gene Transfer Techniques 86
E05.393.385 Genetic Association Studies 270
E05.393.420 Genetic Engineering 520
E05.393.435 Genetic Testing 1 276
E05.393.442 Genotyping Techniques 161
E05.393.475 In Situ Nick-End Labeling 36
E05.393.520 Molecular Diagnostic Techniques 1 031
E05.393.522 Molecular Epidemiology 148
E05.393.542 Molecular Typing 96
E05.393.560 Mutagenicity Tests 441
E05.393.600 Nuclease Protection Assays 1
E05.393.620 Nucleic Acid Amplification Techniques 123
E05.393.640 Nucleic Acid Denaturation 62
E05.393.661 Nucleic Acid Hybridization 172
E05.393.667 Optogenetics 15
E05.393.673 Pedigree 550
E05.393.712 Restriction Mapping 65
E05.393.731 Reverse Genetics 4
E05.393.751 Sequence Alignment 301
E05.393.760 Sequence Analysis 136
E05.393.880 Viral Pseudotyping 1
E05.393.940 Shotgun Sequencing

G Phenomena and Processes
G02 Chemical Phenomena 307
G02.111 Biochemical Phenomena 370
G02.111.007 Acid-Base Equilibrium 444
G02.111.012 Acylation 24
G02.111.017 Aerobiosis 73
G02.111.026 Agglutination 53
G02.111.035 Alkylation 65
G02.111.044 Allosteric Regulation 70
G02.111.053 Amination 7
G02.111.062 Anaerobiosis 143
G02.111.071 Autotrophic Processes 13
G02.111.080 Base Composition 127
G02.111.084 Binding, Competitive 61
G02.111.086 Biocatalysis 82
G02.111.087 Biochemical Processes obsolete
G02.111.092 Biomineralization 4
G02.111.098 Biosynthetic Pathways 50
G02.111.109 Biotinylation 20
G02.111.130 Body Composition 535
G02.111.150 Brain Chemistry 423
G02.111.158 Carbohydrate Metabolism 491
G02.111.165 Citric Acid Cycle 57
G02.111.180 Cyclization 55
G02.111.188 Dealkylation 4
G02.111.192 Deamination 10
G02.111.195 Decarboxylation 17
G02.111.210 DNA Cleavage 15
G02.111.218 DNA Methylation 444
G02.111.222 DNA Repair 472
G02.111.225 DNA Replication 289
G02.111.240 Down-Regulation 300
G02.111.248 Electron Transport 160
G02.111.255 Energy Transfer 322
G02.111.263 Enzyme Activation 380
G02.111.270 Esterification 62
G02.111.323 Halogenation 45
G02.111.375 Heterotrophic Processes 20
G02.111.380 Hydrogenation 24
G02.111.385 Hydroxylation 65
G02.111.490 Kinetics 1 103
G02.111.515 Lipid Peroxidation 759
G02.111.528 Lipogenesis 58
G02.111.534 Lipolysis 181
G02.111.540 Lipoylation 11
G02.111.545 Melanogenesis
G02.111.550 Membrane Fluidity 83
G02.111.560 Molecular Mimicry 38
G02.111.570 Molecular Structure 1 331
G02.111.587 Nitrogen Cycle 6
G02.111.595 Nitrosation 12
G02.111.603 Nucleic Acid Denaturation 62
G02.111.611 Nucleic Acid Hybridization 172
G02.111.619 Nucleic Acid Renaturation 2
G02.111.635 Osmoregulation 11
G02.111.655 Osmosis 77
G02.111.660 Peptide Biosynthesis 43
G02.111.665 Phosphorylation 720
G02.111.669 Phototrophic Processes 25
G02.111.672 Prenylation 14
G02.111.675 Protein Aggregation, Pathological 16
G02.111.679 Protein Binding 1 253
G02.111.688 Protein Folding 107
G02.111.691 Protein Modification, Translational 7
G02.111.694 Protein Multimerization 123
G02.111.700 Protein Stability 94
G02.111.720 Proteolysis 130
G02.111.730 Proteostasis 5
G02.111.740 RNA Cleavage 6
G02.111.750 RNA Folding 5
G02.111.760 RNA Processing, Post-Transcriptional 56
G02.111.770 RNA Replication
G02.111.780 RNA Stability 46
G02.111.810 Sequence Homology 74
G02.111.820 Signal Transduction 2 210
G02.111.830 Structure-Activity Relationship 1 820
G02.111.833 Substrate Cycling 1
G02.111.835 Substrate Specificity 335
G02.111.873 Transcription, Genetic 658
G02.111.905 Up-Regulation 376
G05 Genetic Phenomena 135
G05.045 Biological Evolution 1 257
G05.090 Breeding 146
G05.113 Cell Division 848
G05.135 Cellular Reprogramming 57
G05.158 Clonal Evolution 28
G05.180 Consanguinity 81
G05.193 DNA Cleavage 15
G05.200 DNA Damage 966
G05.206 DNA Methylation 444
G05.213 DNA Packaging 3
G05.219 DNA Repair 472
G05.226 DNA Replication 289
G05.232 DNA Transformation Competence
G05.285 Founder Effect 20
G05.297 Gene Expression 1 552
G05.308 Gene Expression Regulation 1 079
G05.330 Gene Frequency 442
G05.340 Gene Order 43
G05.342 Gene Pool 6
G05.344 Gene Rearrangement 184
G05.346 Genetic Background 13
G05.347 Genetic Fitness 29
G05.348 Genetic Linkage 93
G05.350 Genetic Load 21
G05.355 Genetic Processes obsolete
G05.360 Genetic Structures 25
G05.365 Genetic Variation 861
G05.370 Genomic Instability 88
G05.380 Genotype 1 918
G05.390 Heredity 210
G05.400 Hybrid Vigor 7
G05.410 Inbreeding Depression
G05.420 Inheritance Patterns 50
G05.545 Molecular Mimicry 38
G05.558 Mutagenesis 276
G05.627 Nucleic Acid Denaturation 62
G05.695 Phenotype 2 246
G05.700 Ploidies 90
G05.728 Recombination, Genetic 262
G05.755 RNA Cleavage 6
G05.762 RNA Damage
G05.769 RNA Replication
G05.783 Selection, Genetic 143
G05.810 Sequence Homology 74
G05.813 Sex Determination Processes 58
G05.815 Sex Ratio 96
G05.820 Structural Homology, Protein 30
G05.910 Sympatry 25
G05.935 Virus Integration 32

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