Protein sample preparation is a critical and an unsustainable step since it involves the use of tedious methods that usually require high amount of solvents. The development of new materials offers additional opportunities in protein sample preparation. This work explores, for the first time, the potential application of carboxylate-terminated carbosilane dendrimers to the purification/enrichment of proteins. Studies on dendrimer binding to proteins, based on protein fluorescence intensity and emission wavelengths measurements, demonstrated the interaction between carboxylate-terminated carbosilane dendrimers and proteins at all tested pH levels. Interactions were greatly affected by the protein itself, pH, and dendrimer concentration and generation. Especially interesting was the interaction at acidic pH since it resulted in a significant protein precipitation. Dendrimer-protein interactions were modeled observing stable complexes for all proteins. Carboxylate-terminated carbosilane dendrimers at acidic pH were successfully used in the purification/enrichment of proteins extracted from a complex sample. Graphical Abstract Images showing the growing turbidity of solutions containing a mixture of proteins (lysozyme, myoglobin, and BSA) at different protein:dendrimer ratios (1:0, 1:1, 1:8, and 1:20) at acidic pH and SDS-PAGE profiles of the corresponsing supernatants. Comparison of SDS-PAGE profiles for the pellets obtained during the purification of proteins present in a complex sample using a conventional "no-clean" method based on acetone precipitation and the proposed "greener" method using carboxylate-terminated carbosilane dendrimer at a 1:20 protein:dendrimer ratio.

• MeSH
• chemická precipitace MeSH
• dendrimery chemie   MeSH
• koncentrace vodíkových iontů MeSH
• kyseliny karboxylové chemie   MeSH
• muramidasa chemie   izolace a purifikace   MeSH
• myoglobin chemie   izolace a purifikace   MeSH
• rostlinné proteiny izolace a purifikace   MeSH
• rozpouštědla MeSH
• sekundární struktura proteinů MeSH
• semena rostlinná chemie   MeSH
• sérový albumin hovězí chemie   izolace a purifikace   MeSH
• silany chemie   MeSH
• simulace molekulární dynamiky MeSH
• slivoň švestka chemie   MeSH
• vazba proteinů MeSH
• Publikační typ
• časopisecké články MeSH