URA5-RFLP is one of the most widely used genotyping methods relating to Cryptococcus neoformans and C. gattii consensus genotype nomenclature. In order to identify a molecular type, this method uses a visual comparison of digested PCR products of tested and reference strains, therefore any anomaly in RFLP patterns of studied isolates makes recognition difficult or impossible. This report describes a strain of VNIV type showing an atypical URA5-RFLP pattern as well as a group of AD hybrids displaying the same anomaly. The atypical RFLP pattern is the result of a point mutation and emergence of a new restriction site. Emergence of the allele presenting a new banding pattern may lead to misidentification using the URA5-RFLP technique; the results of this study as well as the literature data may suggest the spread of the allele in the environment.

• MeSH
• Cryptococcus neoformans klasifikace   genetika   MeSH
• genotyp MeSH
• geny hub genetika   MeSH
• mikrobiologie životního prostředí MeSH
• mutace MeSH
• mykologické určovací techniky MeSH
• orotátfosforibosyltransferasa genetika   MeSH
• polymorfismus délky restrikčních fragmentů MeSH
• sekvence nukleotidů MeSH
• Publikační typ
• časopisecké články MeSH