Membrane rafts are microdomains of the plasma membrane that have multiple biological functions. The involvement of these structures in the biology of T cells, namely in signal transduction by the TCR, has been widely studied. However, the role of membrane rafts in immunoreceptor signaling in NK cells is less well known. We studied the distribution of the activating NKG2D receptor in lipid rafts by isolating DRMs in a sucrose density gradient or by raft fractionation by β-OG-selective solubility in the NKL cell line. We found that the NKG2D-DAP10 complex and pVav are recruited into rafts upon receptor stimulation. Qualitative proteomic analysis of these fractions showed that the actin cytoskeleton is involved in this process. In particular, we found that the actin-bundling protein L-plastin plays an important role in the clustering of NKG2D into lipid rafts. Moreover, coengagement of the inhibitory receptor NKG2A partially disrupted NKG2D recruitment into rafts. Furthermore, we demonstrated that L-plastin participates in NKG2D-mediated inhibition of NK cell chemotaxis.
- MeSH
- buněčná membrána účinky léků metabolismus MeSH
- buňky NK cytologie metabolismus MeSH
- centrifugace - gradient hustoty MeSH
- chemotaxe leukocytů fyziologie MeSH
- detergenty farmakologie MeSH
- kultivované buňky MeSH
- lektinové receptory NK-buněk - podrodina C metabolismus MeSH
- lektinové receptory NK-buněk - podrodina K fyziologie MeSH
- lidé MeSH
- malá interferující RNA farmakologie MeSH
- membránové mikrodomény účinky léků fyziologie MeSH
- mikrofilamenta fyziologie MeSH
- mikrofilamentové proteiny antagonisté a inhibitory genetika fyziologie MeSH
- multiproteinové komplexy MeSH
- proteom MeSH
- receptory imunologické metabolismus MeSH
- RNA interference MeSH
- signální transdukce imunologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
