The regulatory functions of glycosaminoglycan hyaluronan (HA) are suggested to be dependent on its molecular weight (MW). Proinflammatory and stimulatory effects are proposed mainly for the low MW HA. However, the complex response of blood phagocytes to HA of different MW is unclear. Herein, the effects of highly purified HA of precisely defined MW (52, 250, and 970 kDa) on human blood phagocytes were tested. All MW HA activated blood phagocytes, including the spontaneous production of ROS, degranulation, and the production of tumor necrosis factor alpha, with low MW HA 52 kDa having the highest potency and high MW HA 970 kDa having the lowest potency. Interestingly, HA inhibited ROS production stimulated by opsonized zymosan particles and, in contrast, potentiated starch-activated ROS production, mostly independent of MW. Data showed a significant effect of HA of different MW on blood phagocytes, including high MW HA.
- MeSH
- antigeny CD11b krev MeSH
- CD antigeny krev MeSH
- degranulace buněk účinky léků MeSH
- fagocyty účinky léků fyziologie MeSH
- GPI-vázané proteiny krev MeSH
- kyselina hyaluronová chemie farmakologie MeSH
- lidé MeSH
- molekulová hmotnost MeSH
- molekuly buněčné adheze krev MeSH
- reaktivní formy kyslíku krev MeSH
- receptory komplementu 3b krev MeSH
- TNF-alfa krev MeSH
- zánět krev etiologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- techniky in vitro MeSH
