BACKGROUND: The invasive benthic round goby (Neogobius melanostomus) is the most successful temperate invasive fish and has spread in aquatic ecosystems on both sides of the Atlantic. Invasive species constitute powerful in situ experimental systems to study fast adaptation and directional selection on short ecological timescales and present promising case studies to understand factors involved the impressive ability of some species to colonize novel environments. We seize the unique opportunity presented by the round goby invasion to study genomic substrates potentially involved in colonization success. RESULTS: We report a highly contiguous long-read-based genome and analyze gene families that we hypothesize to relate to the ability of these fish to deal with novel environments. The analyses provide novel insights from the large evolutionary scale to the small species-specific scale. We describe expansions in specific cytochrome P450 enzymes, a remarkably diverse innate immune system, an ancient duplication in red light vision accompanied by red skin fluorescence, evolutionary patterns of epigenetic regulators, and the presence of osmoregulatory genes that may have contributed to the round goby's capacity to invade cold and salty waters. A recurring theme across all analyzed gene families is gene expansions. CONCLUSIONS: The expanded innate immune system of round goby may potentially contribute to its ability to colonize novel areas. Since other gene families also feature copy number expansions in the round goby, and since other Gobiidae also feature fascinating environmental adaptations and are excellent colonizers, further long-read genome approaches across the goby family may reveal whether gene copy number expansions are more generally related to the ability to conquer new habitats in Gobiidae or in fish.
- MeSH
- genom * MeSH
- ryby genetika fyziologie MeSH
- zavlečené druhy * MeSH
- zvířata MeSH
- zvláštnosti životní historie * MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Microtubules nucleated from an organizing center grow radially in all directions. A new study shows that, to organize those microtubules into arrays of parallel bundles, the kinesin-14 Cik1-Kar3 guides growing microtubule tips along pre-existing microtubules.
- MeSH
- kineziny genetika metabolismus MeSH
- konformace proteinů MeSH
- mikrotubulární proteiny MeSH
- mikrotubuly fyziologie MeSH
- molekulární modely MeSH
- proteinkinasa CDC2 genetika metabolismus MeSH
- proteiny asociované s mikrotubuly MeSH
- regulace genové exprese enzymů MeSH
- vazba proteinů MeSH
- Publikační typ
- časopisecké články MeSH
The delivery of newly synthesized soluble lysosomal hydrolases to the endosomal system is essential for lysosome function and cell homeostasis. This process relies on the proper trafficking of the mannose 6-phosphate receptors (MPRs) between the trans-Golgi network (TGN), endosomes and the plasma membrane. Many transmembrane proteins regulating diverse biological processes ranging from virus production to the development of multicellular organisms also use these pathways. To explore how cell signaling modulates MPR trafficking, we used high-throughput RNA interference (RNAi) to target the human kinome and phosphatome. Using high-content image analysis, we identified 127 kinases and phosphatases belonging to different signaling networks that regulate MPR trafficking and/or the dynamic states of the subcellular compartments encountered by the MPRs. Our analysis maps the MPR trafficking pathways based on enzymes regulating phosphatidylinositol phosphate metabolism. Furthermore, it reveals how cell signaling controls the biogenesis of post-Golgi tubular carriers destined to enter the endosomal system through a SRC-dependent pathway regulating ARF1 and RAC1 signaling and myosin II activity.
- MeSH
- ADP-ribosylační faktor 1 genetika metabolismus MeSH
- buněčná membrána enzymologie MeSH
- endozomy enzymologie MeSH
- fosfatidylinositolfosfáty metabolismus MeSH
- genové regulační sítě MeSH
- HeLa buňky MeSH
- lidé MeSH
- mapy interakcí proteinů MeSH
- rac1 protein vázající GTP genetika metabolismus MeSH
- receptor IGF typ 2 genetika metabolismus MeSH
- regulace genové exprese enzymů MeSH
- RNA interference * MeSH
- shluková analýza MeSH
- signální transdukce MeSH
- skupina kinas odvozených od src-genu genetika metabolismus MeSH
- trans-Golgiho síť enzymologie MeSH
- transfekce MeSH
- transport proteinů genetika MeSH
- vysoce účinné nukleotidové sekvenování metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- audiovizuální média MeSH
- časopisecké články MeSH
- práce podpořená grantem MeSH
