The neurotropic bird schistosome Trichobilharzia regenti possesses papain-like cysteine peptidases which have also been shown to be crucial enzymes in various developmental stages of the related human parasites Schistosoma spp. In this paper, we present data obtained by real-time polymerase chain reaction on the temporal distribution of transcripts of two cathepsins in different developmental stages of T. regenti: cathepsin B1 originally described from the gut lumen of schistosomula with presumptive role in nutrient digestion and cathepsin B2 originally found in penetration glands of cercariae with probable involvement in invasion of the final host. In spite of their mutual resemblance at the sequence level, the mRNA expression profiles clearly show distinct expression of cathepsins B1 and B2 during the development from eggs to cercariae. In the case of both cathepsins, the highest level of transcription was detected in intravertebrate stages. Putative functions of cathepsins B1 and B2 in schistosome developmental stages are discussed.
- MeSH
- hlemýždi MeSH
- izoenzymy genetika metabolismus MeSH
- kachny MeSH
- kathepsin B genetika metabolismus MeSH
- proteiny červů genetika metabolismus MeSH
- Schistosomatidae enzymologie genetika růst a vývoj MeSH
- stadia vývoje MeSH
- stanovení celkové genové exprese MeSH
- vývojová regulace genové exprese MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Trichobilharzia regenti is a neurotropic bird schistosome,causing cercarial dermatitis in humans. In this study, ZAP cDNA expression library from Radix peregra s. lat. hepatopancreases containing intramolluscan stages of T. regenti was constructed and screened using PCR with specific and degenerate primers, designed according to previously described serine and cysteine peptidases of other parasite species. Full-length sequences of cathepsins B1 and L, and two serine peptidases, named RpSP1 and RpSP2, were obtained. The protein-protein BLAST analysis and parallel control reactions with template from hepatopancreases of T. regenti non-infected snails revealed that only cathepsin B1 was of parasite origin. The remaining sequences were derived from the snail intermediate host, which implies that the initial source of parasite mRNA was contaminated by snail tissue. Regardless of this contamination, the cDNA library remains an excellent molecular tool for detection and identification of bioactive molecules in T. regenti cercariae.
- MeSH
- cysteinové endopeptidasy genetika MeSH
- genová knihovna MeSH
- hlemýždi parazitologie MeSH
- kathepsin B genetika MeSH
- kathepsin L MeSH
- kathepsiny genetika MeSH
- molekulární sekvence - údaje MeSH
- polymerázová řetězová reakce MeSH
- proteasy genetika MeSH
- proteiny červů genetika MeSH
- Schistosomatidae enzymologie genetika růst a vývoj MeSH
- sekvenční analýza DNA MeSH
- sekvenční homologie aminokyselin MeSH
- stadia vývoje MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- interakce hostitele a parazita fyziologie genetika MeSH
- kathepsin B MeSH
- kathepsiny genetika izolace a purifikace metabolismus MeSH
- larva parazitologie MeSH
- paraziti MeSH
- Pichia genetika metabolismus MeSH
- ptáci parazitologie MeSH
- Schistosoma japonicum genetika metabolismus parazitologie MeSH
- Schistosoma mansoni genetika metabolismus parazitologie MeSH
- Schistosomatidae metabolismus MeSH
