Interaction between a host cell and pathogen is a permanent event and can have either adverse outcome leading to disease or great benefit for their mutual co-existence. Understanding pathological host-pathogen interaction is a prerequisite for unveiling the strategies of pathogens virulence. A number of methods exist today for deciphering and characterizing host-pathogen interaction. To increase their sensitivity and accuracy, these methods are commonly used in combinations, such as affinity purification and liquid chromatography-mass spectrometry analysis, cross-linking together with liquid chromatography-mass spectrometry analysis, or stable isotope labeling with amino acids in cell culture with affinity purification. In this review, we focus on study of the early interaction time interval when the pathogen binds and invades the host cell and activates sophisticated mechanisms to overcome the host defense barrier. We briefly describe the methods applied in identifying bacterial-host cell protein interactions while emphasizing these methods' various strengths and weaknesses.

• MeSH
• interakce hostitele a patogenu * MeSH
• lidé MeSH
• přenos infekční nemoci MeSH
• vazba proteinů MeSH
• Check Tag
• lidé MeSH

Francisella tularensis is a highly virulent intracellular bacterium and the causative agent of tularemia. The disease is characterized by the suboptimal innate immune response and consequently by the impaired adaptive immunity. The virulence of this pathogen depends on proteins encoded by a genomic island termed the Francisella Pathogenicity Island (FPI). However, the precise biological roles of most of the FPI-encoded proteins remain to be clarified. In this study, we employed stable isotope labeling by amino acids in cell culture (SILAC) in combination with affinity protein purification coupled with liquid chromatography-mass spectrometry to identify potential protein-effector binding pairs for two FPI virulence effectors IglJ and VgrG. Our results may indicate that while the IglJ protein interactions primarily affect mitochondria, the VgrG interactions affect phagosome and/or autophagosome biogenesis via targeting components of the host's exocyst complex.

• MeSH
• adaptivní imunita fyziologie   MeSH
• bakteriální proteiny metabolismus   MeSH
• Francisella tularensis metabolismus   MeSH
• genomové ostrovy * MeSH
• hmotnostní spektrometrie MeSH
• přirozená imunita fyziologie   MeSH
• proteomika MeSH
• regulace genové exprese u bakterií * MeSH
• tularemie mikrobiologie   MeSH
• virulence MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Tularémie, neboli zaječí nemoc, je zoonotické onemocnění vyvolané gramnegativní intracelulární bakterií Francisella tularensis. Vzhledem ke své infekčnosti, poměrně jednoduché kultivaci, absenci humánní vakcíny či možnosti šíření prostřednictvím aerosolu je považována za potenciální biologickou hrozbu. K nakažení jedince však v České republice obvykle dochází bodnutím infikovaného klíštěte, méně často komára anebo přímým kontaktem s kontaminovanou zvěří, popř. konzumací kontaminované vody. Cílem tohoto sdělení je komplexní pohled na tularémii počínaje diagnostikou, klinickými projevy a léčbou, obohacenou o vojenskou perspektivu v otázce zneužití Francisella tularensis jako biologické zbraně.

Tularemia, otherwise known as “rabbit fever”, is a zoonotic disease caused by a gram-negative intracellular bacterium – Francisella tularensis. The species is considered as a potential bioterrorism agent due to its high infectivity, the fact of being relatively easy to culture, the absence of human vaccine, and the potential for spreading through aerosol. In the Czech Republic, infection is usually caused by a tick bite, less frequently by a mosquito bite, direct contact with infected animals, or ingestion of contaminated water. The aim of this review is to provide a comprehensive view of tularemia, its diagnosis, clinical symptoms and treatment, along with the military perspective on a potential risk of F. tularensis to be misused as a biological weapon.

• MeSH
• bioterorismus MeSH
• lidé MeSH
• tularemie * diagnóza   epidemiologie   terapie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• přehledy MeSH

• Publikační typ
• abstrakt z konference MeSH