The effect of hydro-alcoholic extract of Curculigo orchoides on hexavalent chromium (Cr VI) induced toxicity in rats was investigated. Sub-acute toxicity studies were performed by OECD guidelines. K2Cr2O7 (30 mg/kg) was administered to all groups except control group for a period of 28 days by oral gavage. Control group received distilled water; treatment groups received C. orchoides (25, 50 and 100 mg/kg). Cr(VI) administration resulted in up-regulation of serum biochemical parameters such as alanine transaminase, aspartate transaminase, alkaline phosphatase, and tissue biochemical markers viz. lipid peroxidation and protein carbonyl content. C. orchioides (100 mg/kg) significantly decreased these enzyme levels. The activities of anti-oxidant enzymes like superoxide dismutase, catalase and glutathione S-transferase were significantly decreased by Cr(VI) administration (50.7%, 43.7% and 37.9%, respectively). Further, mRNA expression studies and histopathology studies confirmed Cr(VI) toxicity. In all cases, C. orchioides promoted significant restoration of enzyme levels in a dose dependent manner. These results suggest the ameliorating effect of C. orchoides on Cr(VI) induced oxidative stress is probably via, modulation of cytokines, transcription factors and apoptotic genes.
- Klíčová slova
- Curculigo orchoides,
- MeSH
- antioxidancia farmakologie terapeutické užití MeSH
- chrom * toxicita MeSH
- Curculigo * MeSH
- dvojchroman draselný toxicita MeSH
- histologické techniky MeSH
- krysa rodu rattus MeSH
- oxidační stres účinky léků MeSH
- polymerázová řetězová reakce MeSH
- potkani Wistar MeSH
- rostlinné extrakty * farmakologie terapeutické užití MeSH
- stanovení celkové genové exprese MeSH
- testy toxicity metody statistika a číselné údaje MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
