• MeSH
• akční potenciály účinky léků   MeSH
• alkany farmakologie   MeSH
• cerebelární ataxie chemicky indukované   patofyziologie   MeSH
• chinolinové sloučeniny farmakologie   MeSH
• financování organizované MeSH
• krysa rodu rattus MeSH
• metoda terčíkového zámku MeSH
• neurotoxiny farmakologie   MeSH
• nízkovodivostní draslíkové kanály aktivované vápníkem fyziologie   MeSH
• Purkyňovy buňky fyziologie   účinky léků   MeSH
• pyridiny farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH

To understand the contribution of potassium (K+) channels, particularly ?-dendrotoxin (D-type)-sensitive K+ channels (Kv.1, Kv1.2 or Kv1.6 subunits), to the generation of neuronal spike output we must have detailed information of the functional role of these channels in the neuronal membrane. Conventional intracellular recording methods in current clamp mode were used to identify the role of ?-dendrotoxin (?-DTX)-sensitive K+ channel currents in shaping the spike output and modulation of neuronal properties of cerebellar Purkinje neurons (PCs) in slices. Addition of ?-DTX revealed that D-type K+ channels play an important role in the shaping of Purkinje neuronal firing behavior. Repetitive firing capability of PCs was increased following exposure to artificial cerebrospinal fluid (aCSF) containing ?-DTX, so that in response to the injection of 0.6 nA depolarizing current pulse of 600 ms, the number of action potentials insignificantly increased from 15 in the presence of 4-AP to 29 action potentials per second after application of DTX following pretreatment with 4-AP. These results indicate that D-type K+ channels (Kv.1, Kv1.2 or Kv1.6 subunits) may contribute to the spike frequency adaptation in PCs. Our findings suggest that the activation of voltage-dependent K+ channels (D and A types) markedly affect the firing pattern of PCs.

• MeSH
• draslíkové kanály izolace a purifikace   metabolismus   MeSH
• elektrofyziologie metody   přístrojové vybavení   MeSH
• financování organizované využití   MeSH
• kůra mozečku fyziologie   metabolismus   MeSH
• peptidy fyziologie   metabolismus   MeSH
• potkani Sprague-Dawley fyziologie   metabolismus   MeSH
• Purkyňova vlákna fyziologie   metabolismus   MeSH