The present study investigated cardiac function in hearts of mice with total deficiency of the ß1-, ß2- and ß3-adrenoceptors (TKO) in comparison to wildtype mice (WT). We investigated cardiac morphology and echocardiographic function, measured protein expression of Ca2+-regulatory proteins, SERCA 2a activity, myofibrillar function, and performed running wheel tests. Heart weight and heart-to-body weight ratio were significantly smaller in TKO as compared to WT. This was accompanied by a decrease in the size of the cardiomyocytes in TKO. Heart rate and ejection fraction were significantly diminished in TKO as compared to WT. Protein expressions of SERCA 2a, ryanodine receptor and Na+/Ca2+-exchanger were similar in TKO and WT mice, but phospholamban protein expression was increased. PKAdependent phosphorylation of phospholamban at serine 16 was absent and CaMKII-dependent phosphorylation at threonine 17 was decreased in TKO. All alterations were paralleled by a decrease in SERCA 2a-activity. A similar maximal calciumdependent tension but an increased myofibrillar calciumsensitivity was measured in TKO as compared to WT. We did not observe relevant functional impairments of TKO in running wheel tests. In the absence of ß-agonistic stimulation, SERCA 2a activity is mainly regulated by alterations of phospholamban expression and phosphorylation. The decreased SERCA 2a activity following ß-adrenoceptor deficiency may be partly compensated by an increased myofibrillar calcium-sensitivity.

• MeSH
• beta-1-adrenergní receptory genetika   nedostatek   MeSH
• beta-2-adrenergní receptory genetika   nedostatek   MeSH
• beta-3-adrenergní receptory genetika   nedostatek   MeSH
• echokardiografie MeSH
• fosforylace fyziologie   MeSH
• kardiomegalie patofyziologie   ultrasonografie   MeSH
• kondiční příprava zvířat fyziologie   MeSH
• kontrakce myokardu fyziologie   MeSH
• myofibrily fyziologie   MeSH
• myši knockoutované MeSH
• myši MeSH
• proteinkinasy závislé na cyklickém AMP metabolismus   MeSH
• pumpa pro výměnu sodíku a vápníku metabolismus   MeSH
• ryanodinový receptor vápníkového kanálu genetika   nedostatek   MeSH
• sarkoplazmatická Ca2+-ATPáza metabolismus   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH