In Saccharomyces cerevisiae, the Yap family of basic leucine zipper (bZip) proteins contains eight members. The Yap family proteins are implicated in a variety of stress responses; among these proteins, Yap1 acts as a major regulator of oxidative stress responses. However, the functional roles of the remaining Yap family members are poorly understood. To elucidate the function of Yap2, we mined candidate target genes of Yap2 by proteomic analysis. Among the identified genes, FRM2 was previously identified as a target gene of Yap2, which confirmed the validity of our screening method. YNL134C and YDL124W were also identified as candidate Yap2 target genes. These genes were upregulated in strains overexpressing Yap2 and possess Yap2 target sequences in their promoter regions. Furthermore, chromatin immunoprecipitation assays showed that YNL134C and YDL124W have Yap2 binding motif. These data will help to elucidate the functional role of Yap2.
- MeSH
- alkoholoxidoreduktasy biosyntéza genetika MeSH
- chromatinová imunoprecipitace MeSH
- DNA fungální genetika MeSH
- geny hub * MeSH
- oxidoreduktasy biosyntéza genetika MeSH
- promotorové oblasti (genetika) MeSH
- proteomika metody MeSH
- regulace genové exprese u hub * MeSH
- Saccharomyces cerevisiae - proteiny biosyntéza genetika metabolismus MeSH
- Saccharomyces cerevisiae genetika metabolismus MeSH
- transkripční faktory metabolismus MeSH
- vazba proteinů MeSH
- vazebná místa MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
