Developmental processes are closely connected to certain states of epigenetic information which, among others, rely on methylation of chromatin. S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) are key cofactors of enzymes catalyzing DNA and histone methylation. To study the consequences of altered SAH/SAM levels on plant development we applied 9-(S)-(2,3-dihydroxypropyl)-adenine (DHPA), an inhibitor of SAH-hydrolase, on tobacco seeds during a short phase of germination period (6 days). The transient drug treatment induced: (1) dosage-dependent global DNA hypomethylation mitotically transmitted to adult plants; (2) pleiotropic developmental defects including decreased apical dominance, altered leaf and flower symmetry, flower whorl malformations and reduced fertility; (3) dramatic upregulation of floral organ identity genes NTDEF, NTGLO and NAG1 in leaves. We conclude that temporal SAH-hydrolase inhibition deregulated floral genes expression probably via chromatin methylation changes. The data further show that plants might be particularly sensitive to accurate setting of SAH/SAM levels during critical developmental periods.
- MeSH
- adenin analogy a deriváty toxicita MeSH
- adenosylhomocysteinasa antagonisté a inhibitory metabolismus MeSH
- DNA primery genetika MeSH
- epigeneze genetická účinky léků fyziologie MeSH
- klíčení účinky léků fyziologie MeSH
- komplementární DNA genetika MeSH
- květy anatomie a histologie fyziologie MeSH
- metylace DNA MeSH
- neparametrická statistika MeSH
- pyl fyziologie MeSH
- regulace genové exprese u rostlin účinky léků genetika fyziologie MeSH
- rostlinné proteiny metabolismus MeSH
- Southernův blotting MeSH
- tabák enzymologie fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Publikační typ
- abstrakt z konference MeSH
