Sphaeromyxa artedielli sp. n. is described from the gall bladder of the Atlantic hookear sculpin Artediellus atlanticus Jordan et Evermann (Cottidae; type host) from northern Norway. The parasite was also found to infect Triglops murrayi Günther (Cottidae). Spores are produced in disporic pansporoblasts in large flat plasmodia. Spores are straight and fusiform with truncated ends, and measure 16.5-18.7 microm x 4.9-6.2 microm. Valves are thick, striated and suture line is straight. Two equal ovoid polar capsules measure 4.2-6.8 microm x 2.9-4.4 microm and contain irregularly folded polar filaments. Distinctive features include spore shape and size, spore length/width relationship, striated valves, equal polar capsules and a short intercapsular distance. Sphaeromyxa bonaerensis Timi et Sardella, 1998, Sphaeromyxa cannolii Sears, Anderson et Greiner, 2011, and Sphaeromyxa sevastopoli Naidenova, 1970 produce straight spores with truncated ends that are of similar length as those of the new species. Sphaeromyxa cannolii differs in showing smooth spores with unequal polar capsules. The new species differs from S. bonaerensis and S. sevastopoli in significantly wider spores and polar capsules. Sphaeromyxa balbianii Thélohan, 1892, a species originally described with significantly smaller spores than S. artedielli sp. n., has previously been recorded from T. murrayi. We show that S. artedielli sp. n. differs from S. balbianii from the type host Gaidropsarus vulgaris (Cloquet) by its SSU rDNA sequence, and suggest that Atlantic records of Sphaeromyxa spp. from T. murrayi represent S. artedielli sp. n. The closest relative to S. artedielli sp. n. according to the SSU rDNA sequences, S. longa Dunkerly, 1921, differs clearly by spore size and shape. In the SSU rDNA-based phylogenetic analyses, S. artedielli sp. n. groups with other Sphaeromyxa spp. with straight spores and truncated ends in a clade that represents a sister-group to Sphaeromyxa spp. with arcuate spores and rounded ends. Our results indicate that an SSU rDNA pseudogene is present in S. balbianii.
- MeSH
- fylogeneze MeSH
- Myxozoa klasifikace cytologie genetika MeSH
- nemoci ryb epidemiologie parazitologie MeSH
- parazitární nemoci u zvířat epidemiologie parazitologie MeSH
- ribozomální DNA genetika MeSH
- ryby MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Norsko MeSH
A myxosporean producing actinospores of the tetractinomyxon type in Hydroides norvegicus Gunnerus (Serpulidae) in Denmark was identified as a member of the family Parvicapsulidae based on small-subunit ribosomal DNA (SSU rDNA) sequences. Myxosporean samples from various Danish and Norwegian marine fishes were examined with primers that detect the novel myxosporean. Sprattus sprattus (Linnaeus) and Clupea harengus Linnaeus (Teleostei, Clupeidae) were found to be infected. The sequences of this parvicapsulid from these hosts were consistently slightly different (0.8% divergence), but both these genotypes were found in H. norvegicus. Disporic trophozoites and minute spores of a novel myxosporean type were observed in the renal tubules of some of the hosts found infected through PCR. The spores appear most similar to those of species of Gadimyxa Køie, Karlsbakk et Nylund, 2007, but are much smaller. The actinospores of the tetractinomyxon type from H. norvegicus have been described previously. In GenBank, the SSU rDNA sequences of Parvicapsulidae gen. sp. show highest identity (82%) with Parvicapsula minibicornis Kent, Whitaker et Dawe, 1997 infecting salmonids (Oncorhynchus spp.) in fresh water in the western North America. A phylogenetic analysis places P. minibicornis and Parvicapsulidae gen. sp. in a sister clade to the other parvicapsulids (Parvicapsula spp. and Gadimyxa spp.).
- MeSH
- fylogeneze MeSH
- genotyp MeSH
- hostitelská specificita MeSH
- ledviny parazitologie MeSH
- molekulární sekvence - údaje MeSH
- Myxozoa klasifikace genetika růst a vývoj izolace a purifikace MeSH
- nemoci ryb parazitologie MeSH
- parazitární nemoci u zvířat parazitologie MeSH
- Polychaeta parazitologie MeSH
- polymerázová řetězová reakce veterinární MeSH
- ribozomální DNA chemie genetika MeSH
- ryby MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA veterinární MeSH
- sladká voda parazitologie MeSH
- spory ultrastruktura MeSH
- stadia vývoje MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Dánsko MeSH
- Norsko MeSH
A total of 22 specimens of whiting Merlangius merlangus (L.) (Teleostei, Gadidae) from the northern Oresund, Denmark were examined for Myxosporea. Zschokkella hildae Auerbach, 1910 (Myxidiidae), Gadimyxa sp. (Parvicapsulidae) and a species of Bipteria occurred in the renal tubules of 9%, 18% and 68% whiting, respectively. Immature spores of the Bipteria species are very similar to spores of Myxoproteus formosus Kovaleva et Gaevskaya, 1979 originally described from the urinary system of whiting from the Celtic Sea. We therefore consider Bipteria sp. from whiting in Denmark conspecifi c with M. formosus and propose Bipteria formosa (Kovaleva et Gaevskaya, 1979) comb. n. The spore of Bipteria formosa is described in detail and compared with other Bipteria spp. The phylogenetic position of B. formosa, based on partial 18S rDNA sequences, is closest to Leptotheca fugu Tun, Yokoyama, Ogawa et Wakabayashi, 2000 and the Sphaerosporidae.
Sequencing of SSU rDNA showed that actinospores of the tetractinomyxon type, which develop in Chone infundibuliformis Kroyer (Annelida, Polychaeta, Sabellidae) from the northern Oresund, Denmark, are identical with Ceratomyxa auerbachi Kabata, 1962 (Myxozoa, Ceratomyxidae). This myxosporean was found in the gallbladder of the Atlantic herring Clupea harengus L. from the northern Oresund, Denmark, and from the Bergen area, western Norway. The pansporocysts and actinospores of C. auerbachi are described. This is the third elucidated two-host life cycle of a marine myxozoan, and the first involving a marine ceratomyxid.
- MeSH
- Eukaryota cytologie fyziologie izolace a purifikace MeSH
- fylogeneze MeSH
- molekulární sekvence - údaje MeSH
- Polychaeta parazitologie MeSH
- protozoální DNA genetika chemie MeSH
- ribozomální DNA genetika chemie MeSH
- RNA ribozomální 18S genetika MeSH
- ryby parazitologie MeSH
- sekvenční analýza DNA MeSH
- sekvenční homologie nukleových kyselin MeSH
- spory protozoální genetika izolace a purifikace MeSH
- žlučník patofyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Geografické názvy
- Dánsko MeSH
- Norsko MeSH
- MeSH
- finanční podpora výzkumu jako téma MeSH
- nemoci ryb přenos psychologie MeSH
- odběr vzorku krve metody využití MeSH
- pijavice parazitologie MeSH
- Trypanosoma parazitologie růst a vývoj MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Geografické názvy
- Norsko MeSH
