Práca podáva prehľad názorov a informácií o natívnych IVF cykloch a IVF cykloch s ľahkou hormonálnou podporou. Výhodou natívnych IVF cyklov oproti cyklom po kompletnej medikácii je minimálna záťaž pre pacientku, minimálne riziko komplikácií pri odbere oocytu, takmer žiadne riziko ovariálneho hyperstimulačného syndrómu, minimálne riziko viacplodovej gravidity, minimálne alebo žiadne náklady na kryoprezerváciu embryí a podstatne nižšia celková cena cyklu. Nevýhodou natívnych IVF cyklov a IVF cyklov s ľahkou hormonálnou podporou je nižšia úspešnosť.
The article gives an overview of opinions and information about native IVF cycles and IVF cycles with mild hormonal support on the basis of literature data and own experience. First child after extracorporeal fertilization was born in 1978, and was conceived in the native IVF cycle, too. The advantage of native IVF cycles versus IVF cycles after complete medication is the minimal burden for the patient, minimal risk of complications during oocyte collection, almost no risk of ovarian hyperstimulation syndrome, minimal risk of multi-foetal pregnancy, minimal or no cost to the cryopreservation of embryos and significantly lower total price of the cycle. The only disadvantage of native IVF cycles and IVF cycles with mild hormonal support is the lower success rate.
V štúdii sme sa zamerali na porovnanie úspešnosti gonadotropínových preparátov s obsahom luteinizačného hormómu v stimulačných protokoloch v cykloch IVF. V retrospektívnej analýze sme súbor 390 normorespondentných pacientok rozdelili do 4 podskupín v závislosti od použitých gonadotropínových preparátov s obsahom luteinizačnej aktivity (HMG, uFSH, rFSH + HMG, rFSH + rLH). Sledované parametre boli hodnotené u pacientok s vekom do 35 rokov a nad 35 rokov. Protokoly s obsahom luteinizačného hormónu majú vyššiu úspešnosť v porovnaní s protokolmi, v ktorých boli aplikované gonadotropíny so samotným rFSH. U pacientok do 35 rokov stimulovaných gonadotropínovými preparátmi s obsahom luteinizačného hormónu sme v sledovaných ukazovateľoch nezistili významné rozdiely. V skupine nad 35 rokov bol zistený nižší počet tehotností, v ostatných parametroch nebol zistený významný rozdiel.
In this study we focused on comparison of successfulness of gonadotropin preparates with the content of luteinizing hormone in stimulation protocols in IVF cycles. In the retrospective analysis we divided the set of 390 normoresponding patients to 4 groups in dependence on used gonadotropin preparates with the content of luteinizing activity (HMG, uFSH, rFSH+HMG, rFSH+rLH). Monitored parameters were evaluated in patients at the age up to 35 years and over 35 years. The protocols with the content of luteinizing hormone have higher successfulness in comparison with protocols in which gonadotropins with rFSH itself were applied. In patients over 35 years stimulated by gonadotropin preparates with the content of luteinizing hormone we did not find any significant differences in monitored indicators. In the group over 35 years there was spotted a lower number of pregnancies, in the other parameters there was not any significant difference.
Hypogonádotropný hopogonádizmus je charakterizovaný zlyhaním funkcie gonád pri nedostatočnej sekrécii gonádotropínov. V klinickom obraze dominuje pubertas tarda, primárna (sekundárna) amenorea a sterilita. V liečbe sa využívajú exogénne gonádotropíny, inou alternatívou liečby je pulzná aplikácia analógu GnRH. Kazuistika opisuje prípad úspešnej liečby sterility u pacientky s idiopatickou formou hypogonádotropného hypogonádizmu pomocou pulznej aplikácie analógu GnRH po predchádzajúcom zlyhaní liečby exogénnymi gonádotropínmi. Uvedená liečebná modalita predstavuje bezpečnú, dobre tolerovanú a finančne prijateľnú alternatívu stimulácie ovulácie u týchto pacientok.
Hypogonadotropic hypogonadism (HH) characterizes a secondary failure of gonads functions in a deficient gonadotropin secretion. Pubertas tarda, primary (secondary) amenorrhoea and infertility are dominant in the clinical picture. The therapy of these conditions involves application of exogenous gonadotropins or pulsatile GnRH. A case report presents a successful sterility treatment in the patient with a diagnosed idiopathic form of hypogonadotropic hypogonadism with application of pulsatile GnRH after a previous failure of application of exogenous gonadotropins. This therapeutical modality represents a safe, well-tolerated and financially acceptable alternative of ovarian stimulation in such a group of patients.
The aim of our in vitro studies was to understand the role of leptin in controlling proliferation, apoptosis, and protein kinase A (PKA) in human ovarian cells. We analyzed the in vitro effects of leptin (0, 1, 10 or 100 ng/ml) on the accumulation of proliferation-related peptides (PCNA, cyclin B1), apoptosis-associated peptide (Bax) and the intracellular signaling molecule PKA in cultured human granulosa cells using immunocytochemistry and Western immunoblotting. It was observed that leptin stimulated in a dose-dependent manner the accumulation of PCNA (at doses 1-100 ng/ml), cyclin B1 (at doses 10 or 100 ng/ml), Bax (at doses 10 or 100 ng/ml) and PKA (at doses 1-100 ng/ml) in cultured human ovarian cells. These observations suggest the ability of leptin to control directly human ovarian cell functions: proliferation, apoptosis, and intracellular messenger PKA.
- MeSH
- apoptóza MeSH
- buněčný cyklus MeSH
- cyklin B metabolismus MeSH
- dospělí MeSH
- financování organizované MeSH
- folikulární buňky enzymologie imunologie patologie MeSH
- imunohistochemie MeSH
- kultivované buňky MeSH
- leptin metabolismus MeSH
- lidé MeSH
- proliferace buněk MeSH
- proliferační antigen buněčného jádra metabolismus MeSH
- protein X asociovaný s bcl-2 metabolismus MeSH
- proteinkinasy závislé na cyklickém AMP metabolismus MeSH
- rekombinantní proteiny metabolismus MeSH
- signální transdukce MeSH
- western blotting MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
L-carnitine is an amino acid that has been shown to be crucial for the formation of healthy, active sperm. Ferulic acid has been shown to improve sperm quality as well. The antioxidants as vitamin C and vitamin E mixed with tocopherols, green tea extract and selenium improve overall reproductive health which help to improve sperm count and quality. Zinc and vitamin B complex (B6, B12, and folate) are critical nutrients in male reproductive system for adequate hormone metabolism, sperm formation and motility. Material and methods: The main aim of the study was to investigate the effect of nutritional blend on sperm quality in 56 male before and after nutritional treatment. Nutritional treatment lasted three mounths. L-carnitine, L-arginine, vitamin E, coenzyme Q10, selenium, zincum, glutation and folic acid are basic compounds of nutritional supplements that have been studied. Evaluation of sperm quality was performed according to WHO recommendations for semen analysis (1999). Results: 56 patients were divided into three main groups according to the factor of sterility. The first group represents men with oligoasthenoteratozoospermia with total number of sperms under 5 million per mililitre. Patients with oligoasthenoteratozoospermia with total number of sperms over 5 million per mililitre included the second group and the third group included men with asthenoteratozoospermia. The improvement of sperm quality was observed in 12%, 31% and 6% of patients, respectively. The decrease of sperm quality was observed in the second (8%) and the third group (12%). 88% of patients in the first group, 61% of men in the second group and 82% of men in the third group represent men without any change in sperm quality. Conclusions: In conclusion, our results show that nutritional blend has only marginal improvement on sperm quality. It seems that avoidance of stress, enviromental contaminants, smoking, caffeine, drugs and alcohol consumption together (or alone) with nutritional supplementation can enhance sperm production more than nutritional supplementation alone.
