A novel application of a single stranded (ss) oligonucleotide as an active component of polymeric membrane in an ion-selective electrode (ISE) is described. The original oligonucleotides, oligo(dA)(15), modified by cholesterol, triphenylmethyl and hexadecyl derivatives, were immobilized into poly(vinyl chloride) (PVC) membrane using extraction protocol. In parallel, the adsorption protocol was used to immobilize unmodified oligo(dA)(15) on the PVC membrane based on tridodecylmethyammonium chloride (TDDMA(+)Cl(-)). Immobilization of ss oligonucleotide probe through spacer was more effective for the potentiometric detection of the hybridization between complementary oligonucleotides. It was found that cholesterol-oligo(dA)(15) modified membranes were sensitive toward complementary oligo(dT)(15) in the concentration range 2-80 nM at pH 7. An explanation for the detection mechanism is proposed.
- MeSH
- analýza selhání vybavení MeSH
- biokompatibilní potahované materiály chemie MeSH
- biosenzitivní techniky metody přístrojové vybavení MeSH
- design vybavení MeSH
- DNA sondy genetika chemie MeSH
- financování organizované MeSH
- hybridizace in situ metody přístrojové vybavení MeSH
- oligonukleotidy analýza genetika chemie MeSH
- polyvinylchlorid chemie MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- Publikační typ
- hodnotící studie MeSH
