Patient safety is a leading challenge in healthcare and from the laboratory perspective it is now well established that preanalytical errors are the major contributor to the overall rate of diagnostic and therapeutic errors. To address this, the European Federation of Clinical Chemistry and Laboratory Medicine Working Group for Preanalytical Phase (EFLM WG-PRE) was established to lead in standardization and harmonization of preanalytical policies and practices at a European level. One of the key activities of the WG-PRE is the organization of the biennial EFLM-BD conference on the preanalytical phase to provide a forum for National Societies (NS) to discuss their issues. Since 2012, a year after the first Preanalytical phase conference, there has been a rapid growth in the number of NS with a working group engaged in preanalytical phase activities and there are now at least 19 countries that have one. As a result of discussions with NS at the third conference held in March 2015 five key areas were identified as requiring harmonisation. These were test ordering, sample transport and storage, patient preparation, sampling procedures and management of unsuitable specimens. The article below summarises the work that has and will be done in these areas. The goal of this initiative is to ensure the EFLM WG-PRE produces work that meets the needs of the European laboratory medicine community. Progress made in the identified areas will be updated at the next preanalytical phase conference and show that we have produced guidance that has enhanced standardisation in the preanalytical phase and improved patient safety throughout Europe.
- MeSH
- biochemická analýza krve normy MeSH
- klinická chemie organizace a řízení normy MeSH
- lidé MeSH
- referenční standardy MeSH
- směrnice pro lékařskou praxi jako téma MeSH
- společnosti lékařské MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Geografické názvy
- Evropa MeSH
The integrity of whole blood samples may be compromised by suboptimal collection practices. Therefore, we investigated the influence of spurious hyperglycemia on erythrocytes and platelets, assessed with two hematological analyzers using optical or impedance technique. Three K2EDTA blood specimens were collected from 12 healthy subjects, pooled and divided into four aliquots. The first aliquot was left untreated (glucose concentration 4.4 mmol/L), whereas scalar amounts of standard 5% glucose solution were added to the remaining, generating glucose contamination of 5% (19.2 mmol/L), 10% (33 mmol/L) and 20% (62 mmol/L). Hematological testing was then performed using Siemens Advia 2120 and Sysmex XE-2100, whereas glucose and cell-free hemoglobin were measured in centrifuged plasma. Hemolysis did not occur in contaminated aliquots, whereas hemoglobin, erythrocyte count, hematocrit, mean corpuscular hemoglobin concentration and platelet count progressively decreased with glucose concentration. No clinically significant variation was found for red blood cell distribution width and mean corpuscular hemoglobin. The erythrocyte size increased in parallel with glucose, exhibiting larger variation with Advia 2120 than with XE-2100. The mean platelet volume increased on Advia 2120 but not on XE-2100. These results suggest that hyperglycemia induces alterations of erythrocyte and platelet properties, which differ according to the technology used for assessment.
- MeSH
- biomedicínský výzkum metody MeSH
- erytrocyty metabolismus účinky léků MeSH
- hematologické testy * metody normy využití MeSH
- hemoglobiny izolace a purifikace MeSH
- hyperglykemie * MeSH
- krevní glukóza izolace a purifikace MeSH
- lidé MeSH
- odběr vzorku krve * metody přístrojové vybavení využití MeSH
- statistika jako téma MeSH
- trombocyty cytologie metabolismus účinky léků MeSH
- Check Tag
- lidé MeSH
