DNA methylation, one of the most studied epigenetic mechanisms, when present in the promoter region of genes, causes inhibition of gene expression, and conversely, hypomethylation of these regions enables gene expression. DNA methylation is susceptible to nutritional and environmental influences, and undesirable alterations in methylation patterns manifested in changes in the expression of relevant genes can lead to pathological consequences. In the present work, we studied the methylation status of the bovine GSTP1 gene under the influence of pesticide Mospilan 20SP alone and in combination with pesticide Orius 25EW in in vitro proliferating bovine lymphocytes. We employed methylation-specific PCR, and when studying the effect of pesticide combinations, we also used its real-time version followed by a melting procedure. Our results showed that Mospilan 20SP alone at 5, 25, 50, and 100 μg.ml-1 and 5, 10, 25, and 50 μg.ml-1 for the last 4 and 24 hours of culture with in vitro proliferating bovine lymphocytes, respectively, did not induce methylation of the bovine GSTP1 gene. The same results were revealed when studying the effect of the combination of the pesticides added to the lymphocyte cultures for the last 24 hours of cultivation in the following amounts: 1.25, 2.5, 5, 10, and 25 μg.ml-1 of Mospilan 20SP and 1.5, 3, 6, 15, and 30 μg.ml-1 of Orius 25EW. We have also revealed that the less laborious real-time MSP followed by a melting procedure may replace MSP for studying the methylation status of the GSTP1 gene.
The chorioallantoic membrane (CAM) is a highly vascularized avian extraembryonic membrane widely used as an in vivo model to study angiogenesis and its inhibition in response to tissues, cells, or soluble factors. In recent years, the use of CAM has become an integral part of the biocompatibility testing process for developing biomaterials intended for regenerative strategies and tissue engineering applications. In this study, we used the chicken ex ovo CAM assay to investigate the angiogenic potential of innovative acellular biopolymer polyhydroxybutyrate/chitosan (PHB/CHIT) scaffold, which is intended for the treatment of hard tissue defects, depending on treatment with pro- and anti-angiogenic substances. On embryonic day (ED) 7, the experimental biomaterials were placed on the CAM alone or soaked in vascular endothelial growth factor (VEGF-A), saline solution (PHY), or tyrosine kinase inhibitor (SU5402). After 72 h, the formation of vessels was analyzed in the surrounding area of the scaffold and inside the pores of the implants, using markers of embryonic endothelium (WGA, SNA), myofibroblasts (α-SMA), and macrophages (KUL-01). The morphological and histochemical analysis showed strong angiogenic potential of untreated scaffolds without additional effect of the angiogenic factor, VEGF-A. The lowest angiogenic potential was observed in scaffolds soaked with SU5402. Gene expression of pro-angiogenic growth factors, i.e., VEGF-A, ANG-2, and VE-CAD, was upregulated in untreated scaffolds after 72 h, indicating a pro-angiogenic environment. We concluded that the PHB/CHIT has a strong endogenous angiogenic potential and could be promising biomaterial for the treatment of hard tissue defects.
- Publikační typ
- časopisecké články MeSH
The purpose of the study was to determine symptoms related to menopausal status, and their prevalence in two subgroups of the Slovak women divided on the basis of the WHO (1996) definition as pre- and postmenopausal. Two hundred and three women from different regions (Dubnica nad Váhom, Pezinok, Nové Zámky a Bratislava), of non-clinical population, ranging in age betwee n 40 and 59 years were investigated in crosssectional survey. Several aspects of the Menopause-Specific Questionnaire (Kaczmarek, 2000) were examined and compared between the two subgroups of women. Statistical analysis was accomplished with SPSS software (Version 17.0 for Windows) and graphical processing with Excel 2007. The results showed significant differences in issues related to menopausal symptoms, some psychological aspects, intellectual and physical activities between two subgroups of women. Particularly, in the items indicated bothers in night sweats (p < 0.05), ache in muscles and joints (p < 0.01), noticed pins and needles in hands and feet (p < 0.05), vaginal dryness during intercourse (p < 0.05), always with higher mean values (i.e. undesirable) in the postmenopausal women. Higher percentage of the premenopausal women had better sleep, they are more active in intellectual a and physical activities (not specified), than the postmenopausal women. Here we present the first data examining menopausal symptoms in non-clinical sample of women from Slovakia.
- MeSH
- dospělí MeSH
- financování organizované MeSH
- interpretace statistických dat MeSH
- klimakterium fyziologie psychologie MeSH
- kvalita života MeSH
- lidé středního věku MeSH
- lidé MeSH
- menopauza fyziologie psychologie MeSH
- postmenopauza MeSH
- premenopauza MeSH
- průzkumy a dotazníky MeSH
- stárnutí fyziologie psychologie MeSH
- volnočasové aktivity MeSH
- životní styl MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Geografické názvy
- Slovenská republika MeSH
- MeSH
- herpetické infekce prevence a kontrola přenos MeSH
- heterografty * transplantace MeSH
- infekce přenos MeSH
- kmenové buňky cytologie enzymologie MeSH
- lékařská etika MeSH
- lidé MeSH
- nemoci páteře terapie MeSH
- Parkinsonova nemoc terapie MeSH
- rejekce štěpu prevence a kontrola MeSH
- retrovirové infekce prevence a kontrola přenos MeSH
- statistika jako téma MeSH
- transplantace heterologní * metody trendy využití MeSH
- transplantace orgánů * dějiny metody využití MeSH
- Check Tag
- lidé MeSH
