The parasitic gastrointestinal nematode Haemonchus contortus causes serious economic losses to agriculture due to infection and disease in small ruminant livestock. The development of new therapies requires appropriate viability testing, with methods nowadays relying on larval motility or development using procedures that involve microscopy. None of the existing biochemical methods, however, are performed in adults, the target stage of the anthelmintic compounds. Here we present a new test for the viability of H. contortus adults and exsheathed third-stage larvae which is based on a bioluminescent assay of ATP content normalized to total protein concentration measured using bicinchoninic acid. All the procedure steps were optimized to achieve maximal sensitivity and robustness. This novel method can be used as a complementary assay for the phenotypic screening of new compounds with potential antinematode activity in exsheathed third-stage larvae and in adult males. Additionally, it might be used for the detection of drug-resistant isolates.
- MeSH
- adenosintrifosfát terapeutické užití MeSH
- diagnostické techniky molekulární přístrojové vybavení veterinární MeSH
- Haemonchus růst a vývoj izolace a purifikace MeSH
- hemonchóza diagnóza parazitologie veterinární MeSH
- larva růst a vývoj MeSH
- luminiscenční měření přístrojové vybavení veterinární MeSH
- nemoci ovcí diagnóza parazitologie MeSH
- ovce domácí MeSH
- ovce MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
