The parasitic gastrointestinal nematode Haemonchus contortus causes serious economic losses to agriculture due to infection and disease in small ruminant livestock. The development of new therapies requires appropriate viability testing, with methods nowadays relying on larval motility or development using procedures that involve microscopy. None of the existing biochemical methods, however, are performed in adults, the target stage of the anthelmintic compounds. Here we present a new test for the viability of H. contortus adults and exsheathed third-stage larvae which is based on a bioluminescent assay of ATP content normalized to total protein concentration measured using bicinchoninic acid. All the procedure steps were optimized to achieve maximal sensitivity and robustness. This novel method can be used as a complementary assay for the phenotypic screening of new compounds with potential antinematode activity in exsheathed third-stage larvae and in adult males. Additionally, it might be used for the detection of drug-resistant isolates.

• MeSH
• adenosintrifosfát terapeutické užití   MeSH
• diagnostické techniky molekulární přístrojové vybavení   veterinární   MeSH
• Haemonchus růst a vývoj   izolace a purifikace   MeSH
• hemonchóza diagnóza   parazitologie   veterinární   MeSH
• larva růst a vývoj   MeSH
• luminiscenční měření přístrojové vybavení   veterinární   MeSH
• nemoci ovcí diagnóza   parazitologie   MeSH
• ovce domácí MeSH
• ovce MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

This study evaluated patterns and species composition of parasitic infections detected over a 1-year period at an organic goat farm. As a result of coprological examination, the overall prevalence of observed strongylids (99%), coccidia of the genus Eimeria (98%), and Muellerius capillaris lungworms (93%) was calculated. The most prevalent strongylids recovered from incubated fecal samples were Haemonchus contortus (42%), genera Trichostrongylus (23%), Oesophagostomum columbianum (13%), and Teladorsagia circumcincta (11%). A maximum intensity of coccidia infection 5150 oocysts per gram, strongylids infection 9900 eggs per gram and lungworm infection 867.26 larvae per gram were detected. The various effects (including environment, host, and parasites) on milk yield, lactose, protein, and fat were evaluated using generalized linear mixed models. Milk yield (P < 0.0001), milk fat (P < 0.01), and lactose (P < 0.0001) were affected by month, i.e., these parameters were influenced by the month of the year, regardless of the individual goat. With the intensity of infection detected in our study, only protein content was affected (P < 0.01) by parasitic infection (exclusively caused by strongylids). Correlation between measurements from one individual revealed that the goat itself can substantially decrease protein content but has much less of an effect on fat, milk yield, and lactose. Based on our results, we can conclude that a low intensity of parasitic infections does not significantly affect milk yield and the qualitative parameters of milk.

• MeSH
• biozemědělství MeSH
• ezofágostomiáza epidemiologie   veterinární   MeSH
• farmy MeSH
• feces parazitologie   MeSH
• Haemonchus izolace a purifikace   MeSH
• hemonchóza epidemiologie   veterinární   MeSH
• infekce hlísticemi řádu Strongylida epidemiologie   veterinární   MeSH
• kozy parazitologie   MeSH
• Metastrongyloidea izolace a purifikace   MeSH
• mléko sekrece   MeSH
• nemoci koz epidemiologie   parazitologie   MeSH
• Oesophagostomum izolace a purifikace   MeSH
• počet parazitárních vajíček veterinární   MeSH
• roční období MeSH
• trichostrongylóza epidemiologie   veterinární   MeSH
• Trichostrongylus izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH