The study of the complicated architecture of cell space structures is an important problem in biology and medical research. Optical cuts of cells produced by confocal microscopes enable two-dimensional (2D) and three-dimensional (3D) reconstructions of observed cells. This paper discuses new possibilities for direct volume rendering of these data. We often encounter 16 or more bit images in confocal microscopy of cells. Most of the information contained in these images is unsubstantial for the human vision. Therefore, it is necessary to use mathematical algorithms for visualization of such images. Present software tools as OpenGL or DirectX run quickly in graphic station with special graphic cards, run very unsatisfactory on PC without these cards and outputs are usually poor for real data. These tools are black boxes for a common user and make it impossible to correct and improve them. With the method proposed, more parameters of the environment can be set, making it possible to apply 3D filters to set the output image sharpness in relation to the noise. The quality of the output is incomparable to the earlier described methods and is worth increasing the computing time. We would like to offer mathematical methods of 3D scalar data visualization describing new algorithms that run on standard PCs very well.

• MeSH
• biometrie metody   MeSH
• lidé MeSH
• teoretické modely * MeSH
• velikost buňky * MeSH
• zobrazování trojrozměrné metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

This paper discusses new methods for 3-D processing of confocal microscope outputs - single optical cuts through an explored object. The method can model cell illumination by an external light source and is a next important step in the realistic displaying of cells. The computer program based on these methods can be run on a common PC.

• MeSH
• buněčné struktury cytologie   MeSH
• finanční podpora výzkumu jako téma MeSH
• konfokální mikroskopie metody   využití   MeSH
• osvětlení metody   MeSH
• software trendy   MeSH
• zobrazování dat využití   MeSH
• zobrazování trojrozměrné normy   využití   MeSH

• MeSH
• algoritmy MeSH
• buňky ultrastruktura   MeSH
• konfokální mikroskopie * MeSH
• lidé MeSH
• rozpoznávání automatizované MeSH
• zobrazování trojrozměrné * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH

• MeSH
• interpretace obrazu počítačem metody   MeSH
• mikroskopie metody   MeSH
• zobrazování trojrozměrné MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH