- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
petra.kovarikova@faf.cuni.cz Biocompatible iron chelators are currently under extensive investigation as promising drug candidates. Pyridoxal isonicotinoyl hydrazone (PIH) is a lead compound of the aroylhydrazone group of novel iron chelating agents. In this study, the precise and accurate HPLC analytical methods were used for the stability evaluation of water-soluble PIH salt (PIH x 2HCl) in aqueous media of different pH (2.0, 3.9, 7.0, 9.0 and 12.0) as well as in two selected pharmaceutical co-solvents at both laboratory and elevated (40 degrees C) temperatures. The susceptibility of PIH x 2HCl to oxidative decomposition was studied in the solutions of hydrogen peroxide (3 and 30%). Furthermore, the solid substance of PIH x 2HCl was exposed to UV, dry and wet heat. Our experiments revealed that PIH was considerably sensitive to hydrolytic decomposition in aqueous media, resulting in the splitting of the hydrazone bond. The elevated temperature significantly accelerated the hydrolytic reaction. The lowest rate of hydrolysis of PIH was observed in the phosphate buffer of pH 7.0 and in the pharmaceutical co-solvents (30% PEG-300 and 10% Cremophor EL). No special degradation products were detected in the samples exposed to either hydrogen peroxide or co-solvents. The solid substance of PIH x 2HCl was stable when exposed to UV, dry or wet heat for 33 h.
- MeSH
- časové faktory MeSH
- chelátory železa farmakologie MeSH
- chemické modely MeSH
- chemické techniky analytické MeSH
- chemie farmaceutická metody MeSH
- chromatografie MeSH
- financování organizované MeSH
- hydrazony chemie MeSH
- hydrolýza MeSH
- isoniazid analogy a deriváty analýza chemie MeSH
- kalibrace MeSH
- kinetika MeSH
- koncentrace vodíkových iontů MeSH
- kyslík chemie MeSH
- peroxid vodíku chemie MeSH
- pyridoxal analogy a deriváty analýza chemie MeSH
- rozpouštědla chemie MeSH
- teplota MeSH
- ultrafialové záření MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- železo chemie MeSH
- MeSH
- chromatografie kapalinová statistika a číselné údaje MeSH
- elektrochemie MeSH
- hydrolýza metody MeSH
- sulfonamidy analýza MeSH
- Publikační typ
- přehledy MeSH
