The immunocytochemical distribution of three cell surface peptidases was investigated in samples of developing infant breast ranging in age from newborn to 9.5 months. We have previously demonstrated that in the adult breast these enzymes identify subpopulations of epithelial cells and fibroblasts. We therefore wished to address two questions: (a) At what stage in breast development can fibroblast subpopulations be identified, and (b) Is the distribution of these peptidases related to cellular differentiation and morphogenesis? At the histological level there was a cuff of stromal cells closely associated with the developing ductular and lobular structures. At all stages of ductular and lobular development the fibroblasts in this layer were consistently negative for dipeptidyl peptidase IV (DPP IV) and clearly distinguished from the fibroblasts in the surrounding matrix, some of which expressed DPP IV in an age-dependent manner. Within the infant breast aminopeptidase N (APN) was localised to luminal epithelial cells and all fibroblasts, whilst neutral endopeptidase (NEP) was specifically localised to myoepithelial cells. These results are considered in relation to the role of stromal-epithelial interactions during morphogenesis and the proposed function of these enzymes.

• MeSH
• aminopeptidasy analýza   MeSH
• antigeny CD13 MeSH
• dipeptidylpeptidasa 4 MeSH
• dipeptidylpeptidasy a tripeptidylpeptidasy analýza   MeSH
• epitel enzymologie   ultrastruktura   MeSH
• fibroblasty enzymologie   ultrastruktura   MeSH
• kojenec MeSH
• lidé MeSH
• membránové proteiny analýza   MeSH
• morfogeneze MeSH
• neprilysin analýza   MeSH
• novorozenec MeSH
• prsy cytologie   enzymologie   růst a vývoj   MeSH
• svalové proteiny analýza   MeSH
• svaly hladké cévní enzymologie   ultrastruktura   MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Fragments of ductal and lobular epithelium ('organoids') produced by collagenase digestion of reduction-mammoplasty specimens were injected into athymic 'nude' mice. These heterospecific tissues were accepted without rejection, and the presence of xenografts was confirmed by cytology, immunocytochemistry and chromatin staining. Lactation, as confirmed by immunocytochemical and ultrastructural criteria, was observed in the grafted human epithelium during murine pregnancy at both intra- and extra-mammary sites.

• MeSH
• aktiny analýza   MeSH
• buněčná diferenciace MeSH
• buněčné jádro ultrastruktura   MeSH
• chromatin ultrastruktura   MeSH
• dospělí MeSH
• elektronová mikroskopie MeSH
• epitel ultrastruktura   MeSH
• keratiny analýza   MeSH
• kolagen analýza   MeSH
• lidé MeSH
• monoklonální protilátky MeSH
• myši nahé MeSH
• myši MeSH
• prsy cytologie   sekrece   transplantace   MeSH
• těhotenství MeSH
• transplantace heterologní MeSH
• zvířata MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• myši MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH