Molecular cloning, nucleotide sequencing, and characterization of the flaA gene from additional isolates of urease-positive thermophilic Campylobacter (UPTC) were performed. These isolates were obtained from the natural environment in Northern Ireland (n = 9 from mussels) and in England (n = 1 from sea water). All isolates carried the shorter flaA gene, [open reading frames (ORFs), 1,461 to 1,503 base pairs], without any internal termination codons, and did not carry any flaA pseudogenes. The UPTC isolates were well discriminated by the neighbor joining (NJ) phylogenetic tree constructed based on the putative flaA genes ORFs nucleotide sequence information. In addition, the NJ tree constructed based on the flaA-short variable region sequence information discriminated the Campylobacter lari isolates with a similar degree of discrimination power.
- MeSH
- Campylobacter lari klasifikace genetika izolace a purifikace metabolismus MeSH
- Campylobacter klasifikace genetika izolace a purifikace metabolismus MeSH
- flagelin chemie genetika metabolismus MeSH
- fylogeneze MeSH
- klonování DNA MeSH
- mlži mikrobiologie MeSH
- molekulární sekvence - údaje MeSH
- mořská voda mikrobiologie MeSH
- otevřené čtecí rámce MeSH
- polymerázová řetězová reakce MeSH
- rekombinantní proteiny chemie genetika metabolismus MeSH
- sekvence aminokyselin MeSH
- sekvenční analýza DNA metody MeSH
- sekvenční seřazení MeSH
- ureasa metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Anglie MeSH
- Severní Irsko MeSH
