Proteins MiaA and MiaB catalyze sequential isopentenylation and methylthiolation, respectively, of adenosine residue in 37th position of tRNAXXA. The mia mutations were recently shown by us to affect secondary metabolism and morphology of Streptomyces. However, it remained unknown as to whether both or one of the aforementioned modifications is critical for colony development and antibiotic production. Here, we addressed this issue through analysis of Streptomyces albus J1074 strains carrying double miaAmiaB knockout or extra copy of miaB gene. The double mutant differed from wild-type and miaA-minus strains in severity of morphological defects, growth dynamics, and secondary metabolism. Introduction of extra copy of miaB gene into miaA mutant restored aerial mycelium formation to the latter on certain solid media. Hence, miaB gene might be involved in tRNA thiomethylation in the absence of miaA; either MiaA- or MiaB-mediated modification appears to be enough to support normal metabolic and morphological processes in Streptomyces.
- MeSH
- alkyltransferasy a aryltransferasy genetika MeSH
- antibakteriální látky biosyntéza MeSH
- bakteriální geny genetika MeSH
- bakteriální proteiny genetika MeSH
- fenotyp * MeSH
- genetická transkripce MeSH
- genetické testování metody MeSH
- mutace MeSH
- peroxid vodíku farmakologie MeSH
- posttranskripční úpravy RNA * MeSH
- RNA transferová metabolismus MeSH
- sekundární metabolismus účinky léků genetika MeSH
- Streptomyces účinky léků genetika růst a vývoj metabolismus MeSH
- sulfurtransferasy genetika MeSH
- Publikační typ
- časopisecké články MeSH
Streptomyces coelicolor genome carries two apparently paralogous genes, SCO4164 and SCO5854, that encode putative thiosulfate sulfurtransferases (rhodaneses). These genes (and their presumed translation products) are highly conserved and widely distributed across actinobacterial genomes. The SCO4164 knockout strain was unable to grow on minimal media with either sulfate or sulfite as the sole sulfur source. The SCO5854 mutant had no growth defects in the presence of various sulfur sources; however, it produced significantly less amounts of actinorhodin. Furthermore, we discuss possible links between basic interconversions of inorganic sulfur species and secondary metabolism in S. coelicolor.
- MeSH
- anthrachinony metabolismus MeSH
- antibakteriální látky metabolismus MeSH
- bakteriální proteiny genetika metabolismus MeSH
- kultivační média metabolismus MeSH
- sekundární metabolismus MeSH
- sírany metabolismus MeSH
- Streptomyces coelicolor enzymologie genetika růst a vývoj metabolismus MeSH
- thiosulfátsulfurtransferasa genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
Low molecular weight signaling compounds (LMWC) are important players in regulating various aspects of Streptomyces biology. Their exact roles in certain strain will ultimately depend on overall configuration of regulatory network and thus cannot be predicted on basis of in silico studies. Here, we explored S. ghanaensis gene SSFG_07725 (afsAgh) presumably involved in initial steps of formation of γ-butyrolactone LMWC. Disruption of afsAgh impaired aerial mycelium formation and increased the transcription of pleiotropic regulatory gene adpAgh, whereas level of moenomycin production remained virtually unaffected. We provide evidence that morphogenetic deficiency of afsAgh-minus mutant was caused by inability to produce diffusible LMWC. Possible links between γ-butyrolactone signaling and various aspects of S. ghanaensis biology are discussed.
- MeSH
- alkaloidy biosyntéza MeSH
- Bacteria genetika metabolismus MeSH
- biosyntéza peptidů nezávislá na nukleových kyselinách MeSH
- databáze genetické MeSH
- genetické markery MeSH
- houby genetika metabolismus MeSH
- metagenom MeSH
- mezinárodní spolupráce MeSH
- multigenová rodina * MeSH
- peptidy metabolismus MeSH
- polyketidy metabolismus MeSH
- polysacharidy biosyntéza MeSH
- proteosyntéza * MeSH
- rostliny genetika metabolismus MeSH
- terminologie jako téma MeSH
- terpeny metabolismus MeSH
- výpočetní biologie normy MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
Bacteria-assisted bioremediation is widely recognized as a low-cost method to minimize the consequences of soil pollution with toxic metals originating from industrial sites. Strains used in bioremediation have to deal with high metal load via biosorption, reduction, bioprecipitation, metal sequestration, and/or chelation. Actinobacteria, and streptomycetes in particular, are considered a perspective group for bioremediation as natural soil inhabitants with extensive secondary metabolism. Nevertheless, there is no reference information on survival of the model streptomycetes in the presence of the most abundant metal pollutants. Also, there are no reports describing the selection approaches towards improvement of bioremediation properties. In this work, the resistance of Streptomyces coelicolor M145 and Streptomyces sioyaensis Lv81 to certain transition metals and their growth under different pH values are described for the first time. Spontaneous chromate-resistant S. sioyaensis Lv81-138 strain was selected in the course of this work. Strain Lv81-138 is the most efficient actinobacterial Cr(VI) reducer reported so far, capable of converting 12 mmol/L of Cr(VI) into Cr(III) in a medium supplemented with 50 mmol/L K2CrO4.
- MeSH
- bakteriální léková rezistence * MeSH
- biotransformace MeSH
- chromany metabolismus toxicita MeSH
- koncentrace vodíkových iontů MeSH
- kovy metabolismus toxicita MeSH
- kultivační média chemie MeSH
- látky znečišťující životní prostředí metabolismus toxicita MeSH
- mikrobiální viabilita účinky léků MeSH
- mutace * MeSH
- oxidace-redukce MeSH
- Streptomyces účinky léků růst a vývoj metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The cell wall of the model actinomycete Streptomyces coelicolor M145 has recently been shown to contain the novel glycopolymer teichulosonic acid. The major building block of this polymer is 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (Kdn), suggesting initial clues about the genetic control of biosynthesis of this cell wall component. Here, through genome mining and gene knockouts, we demonstrate that the sco4879-sco4882 genomic region of S. coelicolor M145 is necessary for biosynthesis of teichulosonic acid. Specifically, mutants carrying individual knockouts of sco4879, sco4880 and sco4881 genes do not produce Kdn-containing glycopolymer and instead accumulate the minor cell wall component poly(diglycosyl 1-phosphate). Our studies provide evidence that this region is at least partly responsible for biosynthesis of Kdn, whereas flanking genes might control the other steps of teichulosonic acid formation.
- MeSH
- bakteriální polysacharidy biosyntéza MeSH
- buněčná stěna genetika metabolismus MeSH
- data mining MeSH
- DNA bakterií genetika MeSH
- inzerční mutageneze MeSH
- klonování DNA MeSH
- kyseliny cukerné metabolismus MeSH
- magnetická rezonanční spektroskopie MeSH
- Streptomyces coelicolor genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
