DNA replication is a highly coordinated process that is initiated at multiple replication origins in eukaryotes. These origins are bound by the origin recognition complex (ORC), which subsequently recruits the Mcm2-7 replicative helicase in a Cdt1/Cdc6-dependent manner. In budding yeast, two essential replication factors, Sld2 and Mcm10, are then important for the activation of replication origins. In humans, the putative Sld2 homolog, RECQ4, interacts with MCM10. Here, we have identified two mutants of human RECQ4 that are deficient in binding to MCM10. We show that these RECQ4 variants are able to complement the lethality of an avian cell RECQ4 deletion mutant, indicating that the essential function of RECQ4 in vertebrates is unlikely to require binding to MCM10. Nevertheless, we show that the RECQ4-MCM10 interaction is important for efficient replication origin firing.
- MeSH
- apoptóza MeSH
- chromatin genetika MeSH
- helikasy RecQ genetika metabolismus MeSH
- imunoenzymatické techniky MeSH
- imunoprecipitace MeSH
- interakční proteinové domény a motivy MeSH
- kur domácí genetika MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé MeSH
- MCM komplex, komponenta 2 genetika metabolismus MeSH
- MCM komplex, komponenta 7 genetika metabolismus MeSH
- MCM proteiny genetika metabolismus MeSH
- messenger RNA genetika MeSH
- molekulární sekvence - údaje MeSH
- nádorové buňky kultivované MeSH
- nádory kostí genetika metabolismus patologie MeSH
- osteosarkom genetika metabolismus patologie MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- povrchová plasmonová rezonance MeSH
- proliferace buněk MeSH
- průtoková cytometrie MeSH
- replikace DNA * MeSH
- replikační počátek genetika MeSH
- Saccharomyces cerevisiae genetika metabolismus MeSH
- sekvence aminokyselin MeSH
- sekvenční homologie aminokyselin MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
