Here we report the comprehensive characterization of the secondary metabolites from the leaves of Colebrookea oppositifolia Smith, a species used as medicinal plant in the traditional medicine of Nepal. Phytochemical screening of bioactives was performed using an integrated LC-MSn and high resolution MS (Mass Spectrometry) approach. Forty-three compounds were tentatively identified, mainly aglyconic and glycosilated flavonoids and phenolic acids, as well as other bioactives such as coumarins and terpenes were detected. Furthermore, the NF-κB and AP-1 inhibitory activity of C. oppositifolia extract were evaluated, as well as its cytotoxicity against THP-1 cells, in order to assess the potential use of this herb as a source of anti-inflammatory and cytotoxic compounds. The results so far obtained indicate that C. oppositifolia leaves extract could significantly reduce the viability of THP-1 cells (IC50 = 6.2 ± 1.2 µg/mL), as well as the activation of both NF-κB and AP-1 at the concentration of 2 μg/mL. Our results indicate that Nepalese C. oppositifolia is a valuable source of anti-inflammatory and cytotoxic compounds. The phytochemical composition reported here can partially justify the traditional uses of C. oppositifolia in Nepal, especially in the treatment of inflammatory diseases, although further research will be needed to assess the full potential of this species.

• MeSH
• antiflogistika izolace a purifikace   farmakologie   toxicita   MeSH
• chromatografie kapalinová MeSH
• flavonoidy analýza   izolace a purifikace   MeSH
• hluchavkovité metabolismus   MeSH
• hmotnostní spektrometrie MeSH
• hydroxybenzoáty analýza   izolace a purifikace   MeSH
• léčivé rostliny metabolismus   MeSH
• lidé MeSH
• listy rostlin metabolismus   MeSH
• metabolom MeSH
• methanol MeSH
• NF-kappa B antagonisté a inhibitory   MeSH
• rostlinné extrakty chemie   izolace a purifikace   farmakologie   toxicita   MeSH
• THP-1 buňky MeSH
• transkripční faktor AP-1 antagonisté a inhibitory   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Nepál MeSH