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Alija, Avdulla 1 Anderson, Diana 1 Andrade, Vanessa 1 Andreoli, Cristina 1 Asllani, Fisnik 1 Azqueta, Amaya 1 Bangkoglu, Ezgi Eyluel 1 Barančoková, Magdalena 1 Basaran, Nursen 1 Bonassi, Stefano 1 Boutet-Robinet, Elisa 1 Brunborg, Gunnar 1 Bruzzone, Marco 1 Buschini, Annamaria 1 Cavallo, Delia 1 Ceppi, Marcello 1 Collins, Andrew R 1 Costa Pereira, Cristiana 1 Costa, Carla 1 Costa, Solange 1
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ANTICIPE Unit INSERM and University o... 1 Biomedical Sciences Department Univer... 1 Biostatistics Unit San Martino Policl... 1 Cancer Research Institute Biomedical ... 1 Center for Radiation Safety Technolog... 1 Center for RadiationProtection and Hy... 1 Center of Biological Research Faculty... 1 Centre for Molecular and Translationa... 1 Consortium for Biomedical Research in... 1 Department NEUROFARBA University of F... 1 Department of Biochemistry Instituto ... 1 Department of Biology Faculty of Vete... 1 Department of Biology University of P... 1 Department of Chemistry Life Sciences... 1 Department of Environment and Health ... 1 Department of Environmental Health Se... 1 Department of Food Environmental and ... 1 Department of Genetics and Microbiolo... 1 Department of Health Sciences Univers... 1 Department of Nanotoxicolgy and Molec... 1
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Autor
Alija, Avdulla 1 Anderson, Diana 1 Andrade, Vanessa 1 Andreoli, Cristina 1 Asllani, Fisnik 1 Azqueta, Amaya 1 Bangkoglu, Ezgi Eyluel 1 Barančoková, Magdalena 1 Basaran, Nursen 1 Bonassi, Stefano 1 Boutet-Robinet, Elisa 1 Brunborg, Gunnar 1 Bruzzone, Marco 1 Buschini, Annamaria 1 Cavallo, Delia 1 Ceppi, Marcello 1 Collins, Andrew R 1 Costa Pereira, Cristiana 1 Costa, Carla 1 Costa, Solange 1
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Pracoviště
ANTICIPE Unit INSERM and University o... 1 Biomedical Sciences Department Univer... 1 Biostatistics Unit San Martino Policl... 1 Cancer Research Institute Biomedical ... 1 Center for Radiation Safety Technolog... 1 Center for RadiationProtection and Hy... 1 Center of Biological Research Faculty... 1 Centre for Molecular and Translationa... 1 Consortium for Biomedical Research in... 1 Department NEUROFARBA University of F... 1 Department of Biochemistry Instituto ... 1 Department of Biology Faculty of Vete... 1 Department of Biology University of P... 1 Department of Chemistry Life Sciences... 1 Department of Environment and Health ... 1 Department of Environmental Health Se... 1 Department of Food Environmental and ... 1 Department of Genetics and Microbiolo... 1 Department of Health Sciences Univers... 1 Department of Nanotoxicolgy and Molec... 1
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The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.
- MeSH
- biologické markery krev MeSH
- kometový test metody MeSH
- lidé MeSH
- poškození DNA genetika fyziologie MeSH
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- lidé MeSH
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- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
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