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22 záznamů v Medvik Filtry

Článek

Enterococci Isolated from One-Day-Old Chickens and Their Phenotypic Susceptibility to Antimicrobials in the Czech Republic

Bzdil, Jaroslav
Autor Bzdil, Jaroslav ORCID Ptacy S.R.O., Valasska Bystrice 194, 756 27 Valašská Bystřice, Czech Republic
Sladecek, Vladimir
Autor Sladecek, Vladimir Ptacy S.R.O., Valasska Bystrice 194, 756 27 Valašská Bystřice, Czech Republic
Senk, David
Autor Senk, David Ptacy S.R.O., Valasska Bystrice 194, 756 27 Valašská Bystřice, Czech Republic
Stolar, Petr
Autor Stolar, Petr Ptacy S.R.O., Valasska Bystrice 194, 756 27 Valašská Bystřice, Czech Republic
Waicova, Zuzana
Autor Waicova, Zuzana Department of Cell Biology and Genetics, Faculty of Science, Palacký University Olomouc, 17. Listopadu 1192, 779 00 Olomouc, Czech Republic
Kollertova, Nela
Autor Kollertova, Nela Department of Cell Biology and Genetics, Faculty of Science, Palacký University Olomouc, 17. Listopadu 1192, 779 00 Olomouc, Czech Republic
Zouharova, Monika
Autor Zouharova, Monika Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic
Matiaskova, Katarina
Autor Matiaskova, Katarina Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic
Linhart, Petr
Autor Linhart, Petr Institute of Animal Protection and Welfare and Public Veterinary Medicine, University of Veterinary Sciences, Palackeho 1-3, 612 42 Brno, Czech Republic
Nedbalcova, Katerina
Autor Nedbalcova, Katerina Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic

Antibiotics (Basel). 2023 ; 12 (10) : . [pub] 20230927

Antibiotics
ISSN 2079-6382
Medvik
Zdroj

Our study describes the prevalence and spectrum of enterococci isolated from one-day-old chickens in the Czech Republic, their level of antimicrobial resistance, and the occurrence of multiresistance. Over a 24-month period from 1 August 2021 to 31 July 2023, a total of 464 mixed samples of one-day-old chicken organs were examined during routine inspections at 12 randomly selected poultry farms in the Czech Republic. The samples were processed via cultivation methods and suspected strains were confirmed using the MALDI-TOF Mass Spectrometry method. Antimicrobial susceptibility was determined using the MIC method for eight antimicrobials. A total of 128 isolates (prevalence of 27.6%) representing 4 species of enterococci were isolated, including Enterococcus faecalis, Enterococcus faecium, Enterococcus gallinarum, and Enterococcus hirae, with prevalence rates of 23.3%, 1.5%, 2.2%, and 0.6%, respectively. Susceptibility tests showed a high percentage of susceptible strains among E. faecalis, E. faecium, and E. gallinarum for penicillin-based antibiotics, sulfamethoxazole with trimethoprim, and florfenicol (80-100% susceptible strains). E. hirae was an exception, displaying complete resistance to enrofloxacin (0% susceptible strains) and a high degree of resistance to other tested antimicrobials (33.3% susceptible strains). Among the isolated strains, a total of 16 isolates (12.5%) showed resistance to 3 or more antimicrobials. Complete resistance to all eight antimicrobials simultaneously was observed in four isolates (3.1%). This research shows the possible sources of pathogenic enterococci and their virulence and resistance genes. The findings hold relevance for both veterinary and human medicine, contributing to a better understanding of enterococcal circulation in the human ecosystem and food chain, as well as the development of their resistance and multiresistance.

Publikační typ
časopisecké články MeSH

Článek

Oxacillin (Methicillin) Resistant Staphylococci in Domestic Animals in the Czech Republic

Pathogens. 2021 ; 10 (12) : . [pub] 20211206

ISSN 2076-0817
Medvik
Zdroj

The aim of this study was to describe the prevalence of different Staphylococcus species isolated from pathological processes and lesions in domestic animals in the Czech Republic and to detect and describe oxacillin (methicillin)-resistant strains (MRS). During the years 2019-2020, a total of 5218 veterinary clinical samples from the Czech Republic were tested. Testing was performed by culture methods and typing by molecular phenotypic methods MALDI-TOF MS and PCR. Antimicrobial susceptibility testing of the strains was performed by the disk diffusion method. A total of 854 staphylococci strains were identified (16.37% prevalence), out of which 43 strains of 6 species of staphylococci were MRS (n = 43; 0.82% prevalence). Of the MRS strains, the most prevalent species were Staphylococcus pseudintermedius (n = 24; 0.46% prevalence) and Staphylococcus aureus (n = 7; 0.13% prevalence). Susceptibility testing showed resistance to beta-lactam antibiotics and, depending on the species, also to trimethoprim/sulfamethoxazole, gentamicin, tetracycline, erythromycin, clindamycin, and enrofloxacin. For further characterization of MRS, PCR assay for virulence factor genes was performed. Seven of the 14 target genes were observed only in S. aureus, except for the eno gene encoding laminin-binding protein, which was also detected in other staphylococci. It is necessary to emphasize the issue of correct using of antimicrobials in practice and antibiotic policy in university teaching and to create stricter legislation that would prevent the widespread use of antimicrobials in veterinary medicine, especially in livestock to reduce the emergence and spread of antimicrobial resistance.