Biocompatibility tests and a study of the electrical properties of thin films prepared from six electroactive polymer ink formulations based on poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) were performed. The aim was to find a suitable formulation of PEDOT:PSS and conditions for preparing thin films in order to construct printed bioelectronic devices for biomedical applications. The stability and electrical properties of such films were tested on organic electrochemical transistor (OECT)-based sensor platforms and their biocompatibility was evaluated in assays with 3T3 fibroblasts and murine cardiomyocytes. It was found that the thin films prepared from inks without an additive or any thin film post-treatment provide limited conductivity and stability for use in biomedical applications. These properties were greatly improved by using ethylene glycol and thermal annealing. Addition or post-treatment by ethylene glycol in combination with thermal annealing provided thin films with electrical resistance and a stability sufficient to be used in sensing of animal cell physiology. These films coated with collagen IV showed good biocompatibility in the assay with 3T3 fibroblasts when compared to standard cell culture plastics. Selected films were then used in assays with murine cardiomyocytes. We observed that these cells were able to attach to the PEDOT:PSS films and form an active sensor element. Spontaneously beating clusters were formed, indicating a good physiological status for the cardiomyocyte cells. These results open the door to construction of cheap printed electronic devices for biointerfacing in biomedical applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1121-1128, 2018.
- MeSH
- bicyklické sloučeniny heterocyklické chemie MeSH
- biokompatibilní materiály farmakologie MeSH
- buněčné linie MeSH
- buňky 3T3 MeSH
- elektrická impedance MeSH
- elektřina * MeSH
- inkoust * MeSH
- myši MeSH
- polymery chemie MeSH
- polystyreny chemie MeSH
- testování materiálů * MeSH
- voda chemie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Oligonucleotides conduct electric charge via various mechanisms and their characterization and understanding is a very important and complicated task. In this work, experimental (temperature dependent steady state fluorescence spectroscopy, time-resolved fluorescence spectroscopy) and theoretical (Density Functional Theory) approaches were combined to study charge transfer processes in short DNA/DNA and RNA/DNA duplexes with virtually equivalent sequences. The experimental results were consistent with the theoretical model - the delocalized nature of HOMO orbitals and holes, base stacking, electronic coupling and conformational flexibility formed the conditions for more effective short distance charge transfer processes in RNA/DNA hybrids. RNA/DNA and DNA/DNA charge transfer properties were strongly connected with temperature affected structural changes of molecular systems - charge transfer could be used as a probe of even tiny changes of molecular structures and settings.
