A number of cytotoxicity assays are currently available, each of them using specific approach to detect different aspects of cell viability, such as cell integrity, proliferation and metabolic functions. In this study we compared the potential of five commonly employed cytotoxicity assays (WST-1, XTT, MTT, Brilliant blue and Neutral red assay) to detect antiproliferative effects of three selenium compounds, sodium selenite, seleno-L-methionine (SeMet) and Se-(Methyl)selenocysteine (SeMCys) on three colorectal cancer cell lines in vitro. Cells were exposed to the selected selenium compounds in the concentration range of 0-256 microM during 48 h. WST-1 and XTT failed to detect cytotoxic effect, with the exception of the highest concentration of selenium compounds tested. Conversely, the metabolic activity of selenium treated cells measured by WST-1 and XTT significantly increased in comparison to untreated controls. MTT, Neutral red and Brilliant blue assays were more sensitive and yielded mutually comparable results, with significant decrease of measured parameters in a concentration-dependent manner. To a smaller extent, the results were affected by the different chemical nature of the selenium compounds tested as well as by the biological properties of individual cell lines.
- MeSH
- antikarcinogenní látky terapeutické užití toxicita MeSH
- cystein analogy a deriváty terapeutické užití toxicita MeSH
- cytotoxiny terapeutické užití toxicita MeSH
- lidé MeSH
- methionin analogy a deriváty terapeutické užití toxicita MeSH
- nádorové buněčné linie MeSH
- nádory tračníku farmakoterapie metabolismus prevence a kontrola MeSH
- organoselenové sloučeniny terapeutické užití toxicita MeSH
- proliferace buněk účinky léků MeSH
- seleničitan sodný terapeutické užití toxicita MeSH
- testy toxicity metody MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- srovnávací studie MeSH
