Psoriasis is a chronic inflammatory skin disease in which Th17 cells play a crucial role. Since indigenous gut microbiota influences the development and reactivity of immune cells, we analyzed the link among microbiota, T cells and the formation of psoriatic lesions in the imiquimod-induced murine model of psoriasis. To explore the role of microbiota, we induced skin inflammation in germ-free (GF), broad-spectrum antibiotic (ATB)-treated or conventional (CV) BALB/c and C57BL/6 mice. We found that both mice reared in GF conditions for several generations and CV mice treated with ATB were more resistant to imiquimod-induced skin inflammation than CV mice. The ATB treatment dramatically changed the diversity of gut bacteria, which remained stable after subsequent imiquimod application; ATB treatment resulted in a substantial increase in the order Lactobacillales and a significant decrease in Coriobacteriales and Clostridiales. Moreover, as compared to CV mice, imiquimod induced a lower degree of local and systemic Th17 activation in both GF and ATB-treated mice. These findings suggest that gut microbiota control imiquimod-induced skin inflammation by altering the T cell response.
- MeSH
- Actinobacteria účinky léků fyziologie MeSH
- aktivace lymfocytů účinky léků MeSH
- aminochinoliny farmakologie MeSH
- antibakteriální látky farmakologie MeSH
- buňky Th17 účinky léků imunologie mikrobiologie MeSH
- Clostridiales účinky léků fyziologie MeSH
- druhová specificita MeSH
- exprese genu MeSH
- gnotobiologické modely MeSH
- interleukin-17 genetika imunologie MeSH
- jaderné receptory - podrodina 1, skupina F, člen 3 genetika imunologie MeSH
- kůže účinky léků imunologie mikrobiologie patologie MeSH
- Lactobacillales účinky léků fyziologie MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední BALB C MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- psoriáza chemicky indukované imunologie mikrobiologie patologie MeSH
- receptory antigenů T-buněk gama-delta genetika imunologie MeSH
- střevní mikroflóra účinky léků fyziologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
