The aim of the study was to investigate the hyaluronic acid concentration in middle ear fluid of patients with cleft palate as an indicator of the severity of the disease. Hyaluronic acid was examined in the middle ear fluid of 65 children (48 boys and 17 girls) subjected to cleft lip surgery in neonatal period up to 10 days of age. Patients were divided into 3 groups according to the course of the disease. First group consists of 15 patients with favorable course, second group consist of 25 patients with moderate course, third group included 25 patients with an adverse course. Hyaluronic acid levels were determined by commercially available immunoassay. The concentrations of hyaluronic acid in the middle ear fluid were as follows (mean+SEM): favorable course: 14253+2393 μg/l, moderate course: 7503+1345 μg/l, adverse course: 5905+2393 μg/l. Patients with adverse course and moderate course had significantly decreased hyaluronic acid levels in middle ear fluid compared to the patients with favorable course (P=0.02 and P=0.0018). Hyaluronic acid concentration is related to the course of the disease and the lowest values are most frequent in patients with an adverse course.

• MeSH
• imunoanalýza metody   MeSH
• kyselina hyaluronová analýza   MeSH
• lidé MeSH
• novorozenec MeSH
• otitis media s výpotkem komplikace   diagnóza   MeSH
• posouzení stavu pacienta MeSH
• rozštěp patra komplikace   MeSH
• střední ucho chemie   MeSH
• tělesné tekutiny chemie   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

We report a tumor arising in the middle ear of a 65-year-old female patient that was composed of an ovarian-type stroma (OS) and an epithelial component. The tumor consisted of irregular, polypoid masses containing multiple variably sized cystic spaces, which were invariably surrounded by the OS. The cystic spaces were lined by flat, cuboidal, or columnar epithelial cells, in most parts showing mucinous differentiation. The epithelial lining of the cysts strongly expressed cytokeratins AE1-3, CK7, CK8, CK18, CK19, EMA, and S100 protein. The stroma expressed CD34 and smooth muscle actin. No cytological atypia or mitoses were present, and the proliferative activity was less than 1% in both components. The clonality analysis proved the clonal nature of the neoplasm. We believe that this tumor is a new member in the family of neoplasms containing the OS, and therefore we propose the term mixed epithelial and stromal tumor of the middle ear.

• MeSH
• biopsie MeSH
• buňky stromatu chemie   patologie   MeSH
• diagnostické techniky molekulární MeSH
• epitelové buňky chemie   patologie   MeSH
• imunohistochemie MeSH
• lidé MeSH
• nádorové biomarkery analýza   genetika   MeSH
• nádory komplexní a smíšené chemie   genetika   patologie   chirurgie   MeSH
• nádory ucha chemie   genetika   patologie   chirurgie   MeSH
• počítačová rentgenová tomografie MeSH
• proliferace buněk MeSH
• senioři MeSH
• střední ucho chemie   patologie   chirurgie   MeSH
• terminologie jako téma MeSH
• výsledek terapie MeSH
• Check Tag
• lidé MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH