Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus Fragaria thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects F.ananassa and F.vesca plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single F.ananassa host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via Aphisfabae and A.ruborum, further mechanically transmitted to Nicotianaoccidentalis 37B and sub-inoculated to N.benthamiana, N.benthamiana DCL2/4i, N.occidentalis 37B, and Physalisfloridana plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed F.vesca clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus Cytorhabdovirus. Acyrthosiphonmalvae, A.fabae, and A.ruborum were shown to transmit StrV-1 under experimental conditions.
- MeSH
- fylogeneze MeSH
- genom virový MeSH
- jahodník virologie MeSH
- listy rostlin virologie MeSH
- mšice fyziologie virologie MeSH
- nemoci rostlin parazitologie virologie MeSH
- Rhabdoviridae klasifikace genetika izolace a purifikace MeSH
- vysoce účinné nukleotidové sekvenování MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Strawberry crinkle disease is one of the major diseases that threatens strawberry production. Although the biological properties of the agent, strawberry crinkle virus (SCV), have been thoroughly investigated, its complete genome sequence has never been published. Existing RT-PCR-based detection relies on a partial sequence of the L protein gene, presumably the least expressed viral gene. Here, we present complete sequences of two divergent SCV isolates co-infecting a single plant, Fragaria x ananassa cv. Čačanská raná.
- MeSH
- fylogeneze MeSH
- genetická variace MeSH
- genom virový * MeSH
- jahodník virologie MeSH
- mapování chromozomů MeSH
- nemoci rostlin virologie MeSH
- Rhabdoviridae klasifikace genetika izolace a purifikace MeSH
- RNA virová genetika MeSH
- sekvence nukleotidů MeSH
- sekvenování celého genomu MeSH
- virové proteiny genetika MeSH
- Publikační typ
- časopisecké články MeSH
Acta hygienica, epidemiologica et microbiologica, ISSN 0862-5956 příl. č. 6/1977
24 l. ; 29 cm
- MeSH
- infekce viry z čeledi Rhabdoviridae diagnóza prevence a kontrola MeSH
- klinické laboratorní techniky MeSH
- mikrobiologické techniky metody normy využití MeSH
- Rhabdoviridae izolace a purifikace klasifikace patogenita ultrastruktura MeSH
- sérologické testy metody normy využití MeSH
- vzteklina diagnóza epidemiologie patofyziologie prevence a kontrola MeSH
- Publikační typ
- směrnice MeSH
