A multiplex PCR method was developed to characterize the potential variability within the sip gene from bovine isolates of Streptococcus agalactiae. An incomplete sip gene (ncosip) was found in four isolates of S. agalactiae. According to the in silico analysis of the missing region at amino acid level, the N-terminal of surface immunogenic protein (Sip) was found to contain a LysM domain motif; whilst the incomplete Sip (NcoSip) lacked a part of this motif. Immunity elicitation against Sip was confirmed by immunization of mice. This response was partially observed also with NcoSip.
- MeSH
- aminokyselinové motivy MeSH
- antigeny bakteriální genetika chemie imunologie MeSH
- financování organizované MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- protilátky bakteriální krev MeSH
- sekvence aminokyselin MeSH
- Streptococcus agalactiae genetika chemie imunologie MeSH
- streptokokové infekce imunologie mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH