Common bean seed lots collected from different seed dealers and Malawii agriculture station were screened for the presence of Xanthomonas axonopodis pv. phaseoli. In the laboratory the pathogen was isolated following the routine laboratory assay method, i.e. direct plating method using yeast extract-dextrose-calcium carbonate agar medium (YDC). Yellow, convex, mucoid colonies of Xanthomonas were consistently isolated on YDC from seed samples. The presumptive pathogen was confirmed by isolation on semiselective medium, such as mTBM and MD5A. Further, the pathogen was confirmed by biochemical, physiological and, finally, the pathogenicity tests. Five samples out of seven were positive for Xanthomonas. The isolates were found to cause common blight of 3-week-old common bean plants by 7 d after inoculation. Bacteria with the same characteristics as those inoculated were re-isolated from the infected plants.
- MeSH
- bakteriologické techniky metody MeSH
- fazol mikrobiologie MeSH
- kultivační média chemie MeSH
- nemoci rostlin mikrobiologie MeSH
- semena rostlinná mikrobiologie MeSH
- techniky typizace bakterií MeSH
- virulence MeSH
- Xanthomonas axonopodis izolace a purifikace metabolismus patogenita MeSH
- Geografické názvy
- Egypt MeSH
