In higher metazoans, the nuclear hormone receptors activate transcription trough their specific adaptors, nuclear hormone receptor adaptors NCoA, which are absent in lower metazoans. The Nine amino acid TransActivation Domain, 9aaTAD, was reported for a large number of the transcription activators that recruit general mediators of transcription. In this study, we demonstrated that the 9aaTAD from NHR-49 receptor of nematode C.elegans activates transcription as a small peptide. We showed that the ancient 9aaTAD domains are conserved in the nuclear hormone receptors including human HNF4, RARa, VDR and PPARg. Also their small 9aaTAD peptides effectively activated transcription in absence of the NCoA adaptors. We also showed that adjacent H11 domains in ancient and modern hormone receptors have an inhibitory effect on their 9aaTAD function.

• MeSH
• aktivace transkripce * MeSH
• alfa receptor kyseliny retinové chemie   metabolismus   MeSH
• Caenorhabditis elegans chemie   metabolismus   MeSH
• hepatocytární jaderný faktor 4 chemie   metabolismus   MeSH
• lidé MeSH
• molekulární modely MeSH
• peptidy chemie   metabolismus   MeSH
• PPAR gama chemie   metabolismus   MeSH
• proteinové domény MeSH
• proteiny Caenorhabditis elegans chemie   metabolismus   MeSH
• receptory cytoplazmatické a nukleární chemie   metabolismus   MeSH
• receptory kalcitriolu chemie   metabolismus   MeSH
• sekvence aminokyselin MeSH
• sekvenční seřazení MeSH
• signální transdukce MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH