Early changes after radiation exposure may serve as predictors as well as targets for alleviation of radiation-induced injury in the lung. The aim of our study was to examine alterations on the cell and tissue levels in the lung and blood changes of immunological and cytokines profiles induced by ionizing radiation (IR) during the first month after irradiation in the mice experimental model. Female C57BL/6 mice were total body irradiated (TBI) by 8 Gy. Lung tissue samples and blood and were collected 4, 8 and 24 h, 7, 21 and 30 d after TBI. We measured absolute cell counts, cell populations and cytokines profile in the blood and evaluated histopathological analysis in the lung, immunophenotypization of the main lung cell populations and cytokine profiles. In blood, the acute radiation syndrome developed with recovery being observed at 21-30 d, observed by hematological markers. In the lung tissue, a biphasic response occurred. At first, a significant decreased of lymphocytes, resident tissues macrophages and air/tissue ratio associated with increased neutrophils was observed at 8 - 24 h. Subsequently, increase in infiltrating CD4+ T-lymphocytes, neutrophils and resident tissues macrophages and decreased airiness were measured 21 and 30 d after TBI. In summary, our study describes the mechanisms that lung tissue enables to cope with non-lethal injury.
BACKGROUND: Solar light generates inflammatory responses in exposed skin. These effects are generally attri Buted to UV B light. However, skin is expose d to a huge quantum of UVA photons as UVA is a predominant part of sunlight and the radiation used in tanning Beds. We examined the effects of a single exposure to UVA and UV B wave Bands on cytokine levels in skin and plasma, myeloperoxidase (MPO) activity, expression of induci Ble nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in skin. METHODS: Hairless mice were irradiated with either UVA (10 or 20 J/cm²) or UV B (200 or 800 mJ/cm²). The effects were assessed after 4/24 h. Plasma cytokine levels were evaluated using a Bio-Plex cytokine assay. Cytokine, iNOS and COX-2 levels in skin were determined By Western Blot. Skin MPO activity was monitored spectrophotometrically. RESULTS: UV B induced up-regulation of interleukin-1β (IL-1β) and interleukin-6 (IL-6) and decrease in interleukin-10 (IL-10) mainly after 4 h. In contrast, UVA caused increase in levels of tumor necrosis factor-alpha (TNF-α) and IL-6 after 4 h and up-regulated IL-10 and interleukin-12 (IL-12) after 24 h. The increase in MPO activity from infiltrated leucocytes was o Bserved only in UV B irradiated animals. iNOS was up-regulated 4 h after UVA and UV B treatment. No significant effect on COX-2 expression was detected. CONCLUSIONS: UVA and UV B light affected several inflammatory markers. For individual wave Band, changes in plasma parameters did not correlate with those in skin. Thus evaluation of plasma samples cannot simply Be replaced By determination in skin specimens and vice versa.
- MeSH
- časové faktory MeSH
- cyklooxygenasa 2 metabolismus účinky záření MeSH
- cytokiny * krev účinky záření MeSH
- kůže * metabolismus účinky záření MeSH
- myši bezsrsté MeSH
- myši MeSH
- peroxidasa metabolismus účinky záření MeSH
- spektrofotometrie MeSH
- synthasa oxidu dusnatého, typ II metabolismus účinky záření MeSH
- ultrafialové záření škodlivé účinky MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
